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UniProtKB/Swiss-Prot entry P04816


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name LIVK_ECOLI
Primary accession number P04816
Secondary accession numbers Q2M7C1 Q47343
Integrated into Swiss-Prot on August 13, 1987
Sequence was last modified on July 19, 2003 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 91)
Name and origin of the protein
Protein name Leucine-specific-binding protein [Precursor]
Synonyms LS-BP
L-BP
Gene name
Name: livK
OrderedLocusNames: b3458, JW3423
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3891753 [NCBI, ExPASy, EBI, Israel, Japan]
Landick R., Oxender D.L.;
"The complete nucleotide sequences of the Escherichia coli LIV-BP and LS-BP genes. Implications for the mechanism of high-affinity branched-chain amino acid transport.";
J. Biol. Chem. 260:8257-8261(1985).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1002/jcb.240290305; PubMed=4077929 [NCBI, ExPASy, EBI, Israel, Japan]
Antonucci T.K., Landick R., Oxender D.L.;
"The leucine binding proteins of Escherichia coli as models for studying the relationships between protein structure and function.";
J. Cell. Biochem. 29:209-216(1985).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2195019 [NCBI, ExPASy, EBI, Israel, Japan]
Adams M.D., Wagner L.M., Graddis T.J., Landick R., Antonucci T.K., Gibson A.L., Oxender D.L.;
"Nucleotide sequence and genetic characterization reveal six essential genes for the LIV-I and LS transport systems of Escherichia coli.";
J. Biol. Chem. 265:11436-11443(1990).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1093/nar/22.13.2576; PubMed=8041620 [NCBI, ExPASy, EBI, Israel, Japan]
Sofia H.J., Burland V., Daniels D.L., Plunkett G. III, Blattner F.R.;
"Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes.";
Nucleic Acids Res. 22:2576-2586(1994).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[7]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-36.
DOI=10.1073/pnas.77.4.2005; PubMed=6990419 [NCBI, ExPASy, EBI, Israel, Japan]
Oxender D.L., Anderson J.J., Daniels C.J., Landick R., Gunsalus R.P., Zurawski G., Yanofsky C.;
"Amino-terminal sequence and processing of the precursor of the leucine-specific binding protein, and evidence for conformational differences between the precursor and the mature form.";
Proc. Natl. Acad. Sci. U.S.A. 77:2005-2009(1980).
[8]
PROTEIN SEQUENCE OF 24-35.
STRAIN=K12 / EMG2;
DOI=10.1002/elps.1150180807; PubMed=9298646 [NCBI, ExPASy, EBI, Israel, Japan]
Link A.J., Robison K., Church G.M.;
"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.";
Electrophoresis 18:1259-1313(1997).
[9]
X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS).
DOI=10.1016/0022-2836(89)90532-9; PubMed=2649683 [NCBI, ExPASy, EBI, Israel, Japan]
Sack J.S., Trakhanov S.D., Tsigannik I.H., Quiocho F.A.;
"Structure of the L-leucine-binding protein refined at 2.4-A resolution and comparison with the Leu/Ile/Val-binding protein structure.";
J. Mol. Biol. 206:193-207(1989).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M29378; AAA24090.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
J05516; AAA83883.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00039; AAB18433.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC76483.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAE77835.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
V00301; CAA23579.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR E65142; BLECL.
RefSeq AP_004334.1; -.
NP_417915.1; -.
3D structure databases
PDB
1USG; X-ray; 1.53 A; A=24-369.[ExPASy / RCSB / EBI]
1USI; X-ray; 1.80 A; A/C=24-369.[ExPASy / RCSB / EBI]
1USK; X-ray; 2.00 A; A/B/C/D=24-369.[ExPASy / RCSB / EBI]
2LBP; X-ray; 2.40 A; A=24-369.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1USG; -.
1USI; -.
1USK; -.
2LBP; -.
ModBase P04816.
Protein family/group databases
TCDB 3.A.1.4.1; ATP-binding cassette (ABC) superfamily.
Enzyme and pathway databases
BioCyc EcoCyc:LIVK-MON; -.
2D gel databases
SWISS-2DPAGE P04816; -.
2DBase-Ecoli P04816; -.
ECO2DBASE B040.8; 6TH EDITION.
Organism-specific databases
EchoBASE EB0535; -.
EcoGene EG10540; livK.
Ontologies
GO
GO:0030288; Cellular component: outer membrane-bounded periplasmic space (inferred from electronic annotation from InterPro).
GO:0006865; Biological process: amino acid transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001828; ANF_lig_bd_rcpt.
IPR000709; Leu_Ile_Val_bd.
Graphical view of domain structure.
Pfam PF01094; ANF_receptor; 1.
Pfam graphical view of domain structure.
PRINTS PR00337; LEUILEVALBP.
Other
SWISS-3DIMAGE P04816.
Genome annotation databases
GeneID 947964; -.
GenomeReviews AP009048_GR; JW3423.
U00096_GR; b3458.
KEGG ecj:JW3423; -.
eco:b3458; -.
Phylogenomic databases
HOGENOM P04816; -.
OMA P04816; YKLIFRT.
Genome annotation databases
CMR P04816; b3458.
Other
ProtoNet P04816.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Amino-acid transport; Complete proteome; Direct protein sequencing; Disulfide bond; Periplasm; Signal; Transport.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
SIGNAL   1    23  23      
CHAIN   24   369  346     Leucine-specific-binding protein. PRO_0000017702
DISULFID   76   101        By similarity. 
CONFLICT   3     3        R -> A (in Ref. 7; CAA23579). 
CONFLICT   43    43        D -> I (in Ref. 1, 2 and 3). 
CONFLICT   50    50        R -> E (in Ref. 1, 2 and 3). 
CONFLICT   126   126        N -> A (in Ref. 1, 2 and 3). 
STRAND   26    32  7      
STRAND   34    36  3      
HELIX   39    58  20      
TURN   59    61  3      
STRAND   67    73  7      
HELIX   78    90  13      
STRAND   95    97  3      
HELIX   102   115  14      
STRAND   118   121  4      
HELIX   127   130  4      
STRAND   135   139  5      
HELIX   144   146  3      
HELIX   147   157  11      
STRAND   162   168  7      
HELIX   172   187  16      
STRAND   192   197  6      
HELIX   206   214  9      
STRAND   219   224  6      
HELIX   226   238  13      
STRAND   244   247  4      
HELIX   249   251  3      
HELIX   256   260  5      
HELIX   261   264  4      
STRAND   268   271  4      
HELIX   275   277  3      
HELIX   279   281  3      
HELIX   282   290  9      
HELIX   298   317  20      
HELIX   322   332  11      
STRAND   334   336  3      
STRAND   339   341  3      
STRAND   347   351  5      
STRAND   355   359  5      
STRAND   365   367  3      
Sequence information
Length: 369 AA [This is the length of the unprocessed precursor] Molecular weight: 39379 Da [This is the MW of the unprocessed precursor] CRC64: 42B939A18D8723B2 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKRNAKTIIA GMIALAISHT AMADDIKVAV VGAMSGPIAQ WGDMEFNGAR QAIKDINAKG 

        70         80         90        100        110        120 
GIKGDKLVGV EYDDACDPKQ AVAVANKIVN DGIKYVIGHL CSSSTQPASD IYEDEGILMI 

       130        140        150        160        170        180 
SPGATNPELT QRGYQHIMRT AGLDSSQGPT AAKYILETVK PQRIAIIHDK QQYGEGLARS 

       190        200        210        220        230        240 
VQDGLKAANA NVVFFDGITA GEKDFSALIA RLKKENIDFV YYGGYYPEMG QMLRQARSVG 

       250        260        270        280        290        300 
LKTQFMGPEG VGNASLSNIA GDAAEGMLVT MPKRYDQDPA NQGIVDALKA DKKDPSGPYV 

       310        320        330        340        350        360 
WITYAAVQSL ATALERTGSD EPLALVKDLK ANGANTVIGP LNWDEKGDLK GFDFGVFQWH 


ADGSSTAAK 

P04816 in FASTA format

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