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UniProtKB/Swiss-Prot entry P04653

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CASA1_SHEEP
Primary accession number P04653
Secondary accession numbers Q69B23 Q9TS03 Q9TS48
Integrated into Swiss-Prot on August 13, 1987
Sequence was last modified on January 4, 2005 (Sequence version 3)
Annotations were last modified on    June 16, 2009 (Entry version 63)
Name and origin of the protein
Protein name Alpha-S1-casein [Precursor]
Synonyms None
Gene name
Name: CSN1S1
From
Ovis aries (Sheep) [TaxID: 9940] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Caprinae; Ovis.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA] (C ALLELE; ISOFORM 2).
DOI=10.1016/S0300-9084(85)80291-1; PubMed=3002501 [NCBI, ExPASy, EBI, Israel, Japan]
Mercier J.-C., Gaye P., Soulier S., Hue-Delahaie D., Vilotte J.-L.;
"Construction and identification of recombinant plasmids carrying cDNAs coding for ovine alpha S1-, alpha S2-, beta-, kappa-casein and beta-lactoglobulin. Nucleotide sequence of alpha S1-casein cDNA.";
Biochimie 67:959-971(1985).
[2]
 PROTEIN SEQUENCE OF 16-214 (A; C AND D ALLELES; ISOFORMS 1 AND 2), PHOSPHORYLATION AT SER-27; SER-56; SER-61; SER-63; SER-79; SER-80; SER-81; SER-82; SER-83; SER-90 AND SER-130, AND MASS SPECTROMETRY.
TISSUE=Milk;
PubMed=7601973 [NCBI, ExPASy, EBI, Israel, Japan]
Ferranti P., Malorni A., Nitti G., Laezza P., Pizzano R., Chianese L., Addeo F.;
"Primary structure of ovine alpha s1-caseins: localization of phosphorylation sites and characterization of genetic variants A, C and D.";
J. Dairy Res. 62:281-296(1995).
[3]
 VARIANT THR-209.
STRAIN=Comisana, Sarda, and Sopravissana;
TISSUE=Blood;
PubMed=15328285 [NCBI, ExPASy, EBI, Israel, Japan]
Ceriotti G., Chessa S., Bolla P., Budelli E., Bianchi L., Duranti E., Caroli A.;
"Single nucleotide polymorphisms in the ovine casein genes detected by polymerase chain reaction-single strand conformation polymorphism.";
J. Dairy Sci. 87:2606-2613(2004).
Comments
  • FUNCTION: Important role in the capacity of milk to transport calcium phosphate.
  • SUBCELLULAR LOCATION: Secreted.
  • ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
    Name1
    SynonymsLong
    Isoform IDP04653-2
    This is the isoform sequence displayed in this entry.
    Name2
    SynonymsShort
    Isoform IDP04653-1
    Features which should be applied to build the isoform sequence: VSP_012408.
  • TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
  • MASS SPECTROMETRY: Mass=23638.14; Mass_error=3.0; Method=Electrospray; Range=16-214 (P04653-2); Note=Allele A, with 11 phosphate groups; Source=PubMed:7601973;.
  • MASS SPECTROMETRY: Mass=22620.0; Mass_error=1.6; Method=Electrospray; Range=16-214 (P04653-1); Note=Allele A, with 11 phosphate groups; Source=PubMed:7601973;.
  • MASS SPECTROMETRY: Mass=23561.8; Mass_error=2.3; Method=Electrospray; Range=16-214 (P04653-2); Note=Allele C, with 10 phosphate groups; Source=PubMed:7601973;.
  • MASS SPECTROMETRY: Mass=22551.8; Mass_error=2.2; Method=Electrospray; Range=16-214 (P04653-1); Note=Allele C, with 10 phosphate groups; Source=PubMed:7601973;.
  • MASS SPECTROMETRY: Mass=23290.2; Mass_error=2.9; Method=Electrospray; Range=16-214 (P04653-2); Note=Allele D, with 6 phosphate groups; Source=PubMed:7601973;.
  • MASS SPECTROMETRY: Mass=22629.0; Mass_error=1.6; Method=Electrospray; Range=16-214 (P04653-1); Note=Allele D, with 6 phosphate groups; Source=PubMed:7601973;.
  • POLYMORPHISM: At least four alleles are known. The sequence shown is that of allele A. The frequency of variant Thr-209 is 0.263, 0.122 and 0.191 in Italian breeds Comisana, Sarda and Sopravissana, respectively.
  • SIMILARITY: Belongs to the alpha-casein family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X03237; CAA26982.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY444506; AAS17944.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A25069; KASHS1.
RefSeq NP_001009795.1; -.
UniGene Oar.429
3D structure databases
ModBase P04653.
Ontologies
GO
GO:0005576; Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005215; Molecular function: transporter activity (inferred from electronic annotation from InterPro).
GO:0006810; Biological process: transport (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001588; Casein.
Graphical view of domain structure.
Pfam PF00363; Casein; 1.
Pfam graphical view of domain structure.
PROSITE PS00306; CASEIN_ALPHA_BETA; 1.
Genome annotation databases
GeneID 443382; -.
Phylogenomic databases
HOVERGEN P04653; -.
Other
ProtoNet P04653.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Direct protein sequencing; Milk protein; Phosphoprotein; Polymorphism; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    15  15      
CHAIN   16   214  199     Alpha-S1-casein. PRO_0000004459
REGION   105   111  7     Opioid-like peptide sequence. 
MOD_RES   27    27        Phosphoserine; in allele A. 
MOD_RES   56    56        Phosphoserine; in allele C. 
MOD_RES   61    61        Phosphoserine. 
MOD_RES   63    63        Phosphoserine. 
MOD_RES   79    79        Phosphoserine; in alleles A and C. 
MOD_RES   80    80        Phosphoserine. 
MOD_RES   81    81        Phosphoserine; in alleles A and C. 
MOD_RES   82    82        Phosphoserine. 
MOD_RES   83    83        Phosphoserine; in alleles A and C. 
MOD_RES   90    90        Phosphoserine. 
MOD_RES   130   130        Phosphoserine. 
VAR_SEQ   156   163        Missing (in isoform 2). VSP_012408
VARIANT   28    28  1     S -> P (in allele C and allele D). 
VARIANT   83    83  1     S -> N (in allele D). 
VARIANT   209   209  1     I -> T. 
Sequence information
Length: 214 AA [This is the length of the unprocessed precursor] Molecular weight: 24306 Da [This is the MW of the unprocessed precursor] CRC64: 9312FBFFBD6E49DC [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MKLLILTCLV AVALARPKHP IKHQGLSSEV LNENLLRFVV APFPEVFRKE NINELSKDIG 

        70         80         90        100        110        120 
SESIEDQAME DAKQMKAGSS SSSEEIVPNS AEQKYIQKED VPSERYLGYL EQLLRLKKYN 

       130        140        150        160        170        180 
VPQLEIVPKS AEEQLHSMKE GNPAHQKQPM IAVNQELAYF YPQLFRQFYQ LDAYPSGAWY 

       190        200        210 
YLPLGTQYTD APSFSDIPNP IGSENSGKIT MPLW
P04653 in FASTA format

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