ID   MDHM_RAT                Reviewed;         338 AA.
AC   P04636; Q6GSM4;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   09-JAN-2007, sequence version 2.
DT   04-NOV-2008, entry version 85.
DE   RecName: Full=Malate dehydrogenase, mitochondrial;
DE            EC=1.1.1.37;
DE   Flags: Precursor;
GN   Name=Mdh2; Synonyms=Mor1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=86312879; PubMed=3755817; DOI=10.1093/nar/14.15.6053;
RA   Grant P.M., Tellam J., May V.L., Strauss A.W.;
RT   "Isolation and nucleotide sequence of a cDNA clone encoding rat
RT   mitochondrial malate dehydrogenase.";
RL   Nucleic Acids Res. 14:6053-6066(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pituitary anterior lobe;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   PROTEIN SEQUENCE OF 25-338.
RX   MEDLINE=87157605; PubMed=3828294; DOI=10.1021/bi00375a019;
RA   Grant P.M., Roderick S.L., Grant G.A., Banaszak L.J., Strauss A.W.;
RT   "Comparison of the precursor and mature forms of rat heart
RT   mitochondrial malate dehydrogenase.";
RL   Biochemistry 26:128-134(1987).
RN   [4]
RP   PROTEIN SEQUENCE OF 27-52; 53-74; 166-185; 192-203; 216-239; 242-296
RP   AND 308-324, AND MASS SPECTROMETRY.
RC   STRAIN=Sprague-Dawley; TISSUE=Brain, Hippocampus, and Spinal cord;
RA   Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U.;
RL   Submitted (JUL-2007) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = oxaloacetate + NADH.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. MDH type 1 family.
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DR   EMBL; X04240; CAA27812.1; -; mRNA.
DR   EMBL; BC063165; AAH63165.1; -; mRNA.
DR   PIR; A25509; DERTMM.
DR   RefSeq; NP_112413.2; -.
DR   UniGene; Rn.1011; -.
DR   HSSP; P00346; 1MLD.
DR   SMR; P04636; 25-337.
DR   Rat-heart-2DPAGE; P04636; -.
DR   Ensembl; ENSRNOG00000001440; Rattus norvegicus.
DR   GeneID; 81829; -.
DR   KEGG; rno:81829; -.
DR   NMPDR; fig|10116.3.peg.8382; -.
DR   RGD; 619719; Mor1.
DR   HOVERGEN; P04636; -.
DR   NextBio; 615775; -.
DR   ArrayExpress; P04636; -.
DR   GermOnline; ENSRNOG00000001440; Rattus norvegicus.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR001557; L-lactate/malate_DHase.
DR   InterPro; IPR001236; Lactate/malate_DHase.
DR   InterPro; IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
DR   InterPro; IPR001252; Malate_DHase_AS.
DR   InterPro; IPR010097; Malate_DHase_NAD-dep_euk_g_bac.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11540:SF1; MDH_euk_g_bac; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   TIGRFAMs; TIGR01772; MDH_euk_gproteo; 1.
DR   PROSITE; PS00068; MDH; 1.
PE   1: Evidence at protein level;
KW   Acetylation; Direct protein sequencing; Mitochondrion; NAD;
KW   Oxidoreductase; Phosphoprotein; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT       1     24       Mitochondrion.
FT   CHAIN        25    338       Malate dehydrogenase, mitochondrial.
FT                                /FTId=PRO_0000018631.
FT   NP_BIND      31     37       NAD (By similarity).
FT   NP_BIND     140    142       NAD (By similarity).
FT   ACT_SITE    200    200       Proton acceptor (By similarity).
FT   BINDING      57     57       NAD (By similarity).
FT   BINDING     104    104       Substrate (By similarity).
FT   BINDING     110    110       Substrate (By similarity).
FT   BINDING     117    117       NAD (By similarity).
FT   BINDING     142    142       Substrate (By similarity).
FT   BINDING     176    176       Substrate (By similarity).
FT   BINDING     251    251       NAD (By similarity).
FT   MOD_RES      56     56       Phosphotyrosine (By similarity).
FT   MOD_RES     157    157       N6-acetyllysine (By similarity).
FT   MOD_RES     239    239       N6-acetyllysine (By similarity).
FT   MOD_RES     314    314       N6-acetyllysine (By similarity).
FT   CONFLICT    229    229       R -> K (in Ref. 1; CAA27812).
SQ   SEQUENCE   338 AA;  35684 MW;  13849CCFB78C87E7 CRC64;
     MLSALARPVG AALRRSFSTS AQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH
     TPGVAADLSH IETRANVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI
     VATLTAACAQ HCPEAMICII SNPVNSTIPI TAEVFKKHGV YNPNKIFGVT TLDIVRANTF
     VAELKGLDPA RVNVPVIGGH AGKTIIPLIS QCTPKVDFPQ DQLATLTGRI QEAGTEVVKA
     KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVIECSFVQS KETECTYFST PLLLGKKGLE
     KNLGIGKITP FEEKMIAEAI PELKASIKKG EDFVKNMK
//