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UniProtKB/Swiss-Prot entry P04627


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ARAF_MOUSE
Primary accession number P04627
Secondary accession numbers Q99J44 Q9CTT5 Q9D6R6 Q9DBU7
Integrated into Swiss-Prot on August 13, 1987
Sequence was last modified on August 2, 2002 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 104)
Name and origin of the protein
Protein name A-Raf proto-oncogene serine/threonine-protein kinase
Synonym EC 2.7.11.1
Gene name
Name: Araf
Synonyms: A-raf, Araf1
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Mammary gland;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-283.
STRAIN=C57BL/6J;
TISSUE=Lung, Tongue, and Urinary bladder;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[3]
NUCLEOTIDE SEQUENCE [MRNA] OF 168-604.
PubMed=3491291 [NCBI, ExPASy, EBI, Israel, Japan]
Huleihel M., Goldsborough M., Cleveland J., Gunnell M., Bonner T., Rapp U.R.;
"Characterization of murine A-raf, a new oncogene related to the v-raf oncogene.";
Mol. Cell. Biol. 6:2655-2662(1986).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
BC004757; AAH04757.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK004741; BAB23522.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK010060; BAB26674.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK020547; BAB32131.3; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D00024; BAA00018.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A25382; TVMSRF.
RefSeq NP_033833.1; -.
UniGene Mm.220946
3D structure databases
HSSP P04049; 1FAR. [HSSP ENTRY / PDB]
SMR P04627; 20-91, 96-146, 301-574.
ModBase P04627.
Protein-protein interaction databases
DIP DIP:1070N; -.
IntAct P04627; -.
PTM databases
PhosphoSite P04627; -.
Organism-specific databases
MGI MGI:88065; Araf.
Gene expression databases
ArrayExpress P04627; -.
CleanEx MM_ARAF; -.
GermOnline ENSMUSG00000001127; Mus musculus.
Ontologies
GO
GO:0005739; Cellular component: mitochondrion (inferred from direct assay from MGI).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from MGI).
QuickGo view.
Family and domain databases
InterPro IPR002219; DAG_PE_bd.
IPR000719; Prot_kinase_core.
IPR017441; Protein_kinase_ATP_bd_CS.
IPR003116; Raf_like_ras_bd.
IPR017442; Se/Thr_pkinase-rel.
IPR008271; Ser_thr_pkin_AS.
Graphical view of domain structure.
Pfam PF00130; C1_1; 1.
PF00069; Pkinase; 1.
PF02196; RBD; 1.
Pfam graphical view of domain structure.
PRINTS PR00008; DAGPEDOMAIN.
ProDom PD000001; Prot_kinase; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00109; C1; 1.
SM00455; RBD; 1.
SMART graphical view of domain structure.
PROSITE PS00107; PROTEIN_KINASE_ATP; 1.
PS50011; PROTEIN_KINASE_DOM; 1.
PS00108; PROTEIN_KINASE_ST; 1.
PS50898; RBD; 1.
PS00479; ZF_DAG_PE_1; 1.
PS50081; ZF_DAG_PE_2; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P04627.
Genome annotation databases
Ensembl ENSMUSG00000001127; Mus musculus. [Contig view]
GeneID 11836; -.
KEGG mmu:11836; -.
Phylogenomic databases
HOGENOM P04627; -.
HOVERGEN P04627; -.
Other
SOURCE Araf; Mus musculus.
ProtoNet P04627.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Kinase; Metal-binding; Nucleotide-binding; Phorbol-ester binding; Phosphoprotein; Proto-oncogene; Serine/threonine-protein kinase; Transferase; Zinc; Zinc-finger.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   604  604     A-Raf proto-oncogene serine/threonine-protein kinase. PRO_0000085623
DOMAIN   19    91  73     RBD. 
DOMAIN   308   568  261     Protein kinase. 
ZN_FING   98   144  47     Phorbol-ester/DAG-type. 
NP_BIND   314   322  9     ATP (By similarity). 
ACT_SITE   427   427        Proton acceptor (By similarity). 
METAL   99    99        Zinc 1 (By similarity). 
METAL   112   112        Zinc 2 (By similarity). 
METAL   115   115        Zinc 2 (By similarity). 
METAL   125   125        Zinc 1 (By similarity). 
METAL   128   128        Zinc 1 (By similarity). 
METAL   133   133        Zinc 2 (By similarity). 
METAL   136   136        Zinc 2 (By similarity). 
METAL   144   144        Zinc 1 (By similarity). 
BINDING   334   334        ATP (By similarity). 
MOD_RES   213   213        Phosphothreonine (By similarity). 
MOD_RES   215   215        Phosphothreonine (By similarity). 
MOD_RES   255   255        Phosphoserine (By similarity). 
MOD_RES   580   580        Phosphoserine (By similarity). 
CONFLICT   169   169        E -> K (in Ref. 3; BAA00018). 
CONFLICT   186   186        S -> R (in Ref. 2; BAB23522/BAB26674). 
CONFLICT   326   326        R -> L (in Ref. 3; BAA00018). 
Sequence information
Length: 604 AA [This is the length of the unprocessed precursor] Molecular weight: 67581 Da [This is the MW of the unprocessed precursor] CRC64: 05F8262F99DDD087 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEPPRGPPVS GAEPSRAVGT VKVYLPNKQR TVVTVREGMS VYDSLDKALK VRGLNQDCCV 

        70         80         90        100        110        120 
VYRLIKGRKT VTAWDTAIAP LDGEELIVEV LEDVPLTMHN FVRKTFFSLA FCDFCLKFLF 

       130        140        150        160        170        180 
HGFRCQTCGY KFHQHCSSKV PTVCVDMSTN RRQFYHSIQD LSGGSRQQEA PSNLSVNELL 

       190        200        210        220        230        240 
TPQGPSPFTQ QRDQEHFSFP APANPPLQRI RSTSTPNVHM VSTTAPMDSS LMQFTAQSFS 

       250        260        270        280        290        300 
TDAAGRGGDG APRGSPSPAS VSSGRKSPHS KLPSEQRERK SLADEKKKVK NLGYRDSGYY 

       310        320        330        340        350        360 
WEVPPSEVQL LKRIGTGSFG TVFRGRWHGD VAVKVLKVAQ PTAEQAQAFK NEMQVLRKTR 

       370        380        390        400        410        420 
HVNILLFMGF MTRPGFAIIT QWCEGSSLYH HLHVADTRFD MVQLIDVARQ TAQGMDYLHA 

       430        440        450        460        470        480 
KNIIHRDLKS NNIFLHEGLT VKIGDFGLAT VKTRWSGAQP LEQPSGSVLW MAAEVIRMQD 

       490        500        510        520        530        540 
PNPYSFQSDV YAYGVVLYEL MTGSLPYSHI GSRDQIIFMV GRGYLSPDLS KIFSNCPKAM 

       550        560        570        580        590        600 
RRLLTDCLKF QREERPLFPQ ILATIELLQR SLPKIERSAS EPSLHRTQAD ELPACLLSAA 


RLVP 

P04627 in FASTA format

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