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UniProtKB/Swiss-Prot entry P04385


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GAL1_YEAST
Primary accession number P04385
Secondary accession numbers None
Integrated into Swiss-Prot on March 20, 1987
Sequence was last modified on January 23, 2007 (Sequence version 4)
Annotations were last modified on    November 4, 2008 (Entry version 88)
Name and origin of the protein
Protein name Galactokinase
Synonyms EC 2.7.1.6
Galactose kinase
Gene name
Name: GAL1
OrderedLocusNames: YBR020W
ORFNames: YBR0302
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Carlsbergensis;
PubMed=6715281 [NCBI, ExPASy, EBI, Israel, Japan]
Citron B.A., Donelson J.E.;
"Sequence of the Saccharomyces GAL region and its transcription in vivo.";
J. Bacteriol. 158:269-278(1984).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1002/yea.320100010; PubMed=8091864 [NCBI, ExPASy, EBI, Israel, Japan]
Smits P.H.M., de Haan M., Maat C., Grivell L.A.;
"The complete sequence of a 33 kb fragment on the right arm of chromosome II from Saccharomyces cerevisiae reveals 16 open reading frames, including ten new open reading frames, five previously identified genes and a homologue of the SCO1 gene.";
Yeast 10:S75-S80(1994).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=7813418 [NCBI, ExPASy, EBI, Israel, Japan]
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.;
"Complete DNA sequence of yeast chromosome II.";
EMBO J. 13:5795-5809(1994).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-29.
PubMed=6092912 [NCBI, ExPASy, EBI, Israel, Japan]
Johnston M., Davis R.W.;
"Sequences that regulate the divergent GAL1-GAL10 promoter in Saccharomyces cerevisiae.";
Mol. Cell. Biol. 4:1440-1448(1984).
[5]
PROTEIN SEQUENCE OF 2-12.
DOI=10.1016/0014-5793(77)80638-8; PubMed=200486 [NCBI, ExPASy, EBI, Israel, Japan]
Schlesinger D.H., Schell M.A., Wilson D.B.;
"The NH2-terminal sequences of galactokinase from Escherichia coli and Saccharomyces cerevisiae.";
FEBS Lett. 83:45-47(1977).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-349, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
K01609; AAA34631.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X76078; CAA53677.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z35889; CAA84962.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K02115; AAA34621.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S45876; KIBYGG.
RefSeq NP_009576.1; -.
3D structure databases
PDB
2AJ4; X-ray; 2.40 A; A/B=1-528.[ExPASy / RCSB / EBI]
PDBsum 2AJ4; -.
ModBase P04385.
Protein-protein interaction databases
DIP DIP:2333N; -.
IntAct P04385; -.
Organism-specific databases
CYGD YBR020w; -.
SGD S000000224; GAL1.
Yeast-GFP YBR020W.
Gene expression databases
ArrayExpress P04385; -.
GermOnline YBR020W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from genetic interaction from SGD).
GO:0004335; Molecular function: galactokinase activity (inferred from direct assay from SGD).
GO:0046835; Biological process: carbohydrate phosphorylation (inferred from direct assay from SGD).
GO:0033499; Biological process: galactose catabolic process via UDP-galactose (inferred from direct assay from SGD).
GO:0015757; Biological process: galactose transport (inferred from mutant phenotype from SGD).
GO:0000411; Biological process: positive regulation of transcription by galactose (inferred from genetic interaction from SGD).
QuickGo view.
Family and domain databases
InterPro IPR000705; Galactokinase.
IPR006204; GHMP_kinase.
IPR013750; GHMP_kinase_C.
IPR006203; GHMP_knse_ATP_bd_CS.
IPR006206; Mev_galkinase.
IPR014721; Ribosomal_S5_D2-type_fold.
Graphical view of domain structure.
Gene3D G3DSA:3.30.230.10; Ribosomal_S5_D2-type_fold; 1.
Pfam PF08544; GHMP_kinases_C; 1.
PF00288; GHMP_kinases_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000530; Galactokinase; 1.
PRINTS PR00473; GALCTOKINASE.
PR00959; MEVGALKINASE.
TIGRFAMs TIGR00131; gal_kin; 1.
PROSITE PS00106; GALACTOKINASE; 1.
PS00627; GHMP_KINASES_ATP; 1.
BLOCKS P04385.
ProtoNet P04385.
Proteomic databases
PeptideAtlas P04385; -.
Genome annotation databases
Ensembl YBR020W; Saccharomyces cerevisiae. [Contig view]
GeneID 852308; -.
GenomeReviews Y13134_GR; YBR020W.
KEGG sce:YBR020W; -.
NMPDR fig|4932.3.peg.271; -.
Phylogenomic databases
HOGENOM P04385; -.
Other
LinkHub P04385; -.
NextBio 970984; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; ATP-binding; Carbohydrate metabolism; Complete proteome; Direct protein sequencing; Galactose metabolism; Kinase; Nucleotide-binding; Phosphoprotein; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   528  527     Galactokinase. PRO_0000184657
NP_BIND   163   173  11     ATP (Potential). 
MOD_RES   349   349        Phosphoserine. 
CONFLICT   6     6        S -> R (in Ref. 4). 
CONFLICT   162   162        V -> I (in Ref. 1; AAA34631). 
CONFLICT   206   239        EHYVGVNNGGMDQAASVCGEEDHALYVEFKPQLK -> DIMLVLTMAVWIRLPLFAVRKIMLYTLSSNAVE (in Ref. 1). 
CONFLICT   297   297        L -> P (in Ref. 1; AAA34631). 
CONFLICT   428   428        A -> V (in Ref. 1; AAA34631). 
CONFLICT   483   483        N -> NN (in Ref. 1; AAA34631). 
HELIX   23    40  18      
STRAND   45    57  13      
HELIX   62    64  3      
STRAND   68    84  17      
STRAND   90    97  8      
STRAND   103   106  4      
HELIX   123   141  19      
HELIX   143   146  4      
STRAND   147   149  3      
STRAND   153   160  8      
STRAND   166   168  3      
HELIX   170   187  18      
HELIX   195   202  8      
HELIX   203   209  7      
HELIX   216   223  8      
STRAND   229   233  5      
STRAND   235   237  3      
STRAND   239   243  5      
STRAND   252   259  8      
TURN   266   273  8      
HELIX   274   291  18      
HELIX   310   322  13      
HELIX   332   350  19      
HELIX   351   353  3      
HELIX   359   366  8      
HELIX   370   378  9      
STRAND   379   381  3      
HELIX   390   412  23      
HELIX   419   439  21      
HELIX   446   457  12      
STRAND   461   465  5      
STRAND   470   481  12      
HELIX   486   496  11      
HELIX   498   501  4      
HELIX   507   513  7      
STRAND   514   517  4      
STRAND   523   527  5      
Sequence information
Length: 528 AA [This is the length of the unprocessed precursor] Molecular weight: 57944 Da [This is the MW of the unprocessed precursor] CRC64: 1DA31A1915C19107 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MTKSHSEEVI VPEFNSSAKE LPRPLAEKCP SIIKKFISAY DAKPDFVARS PGRVNLIGEH 

        70         80         90        100        110        120 
IDYCDFSVLP LAIDFDMLCA VKVLNEKNPS ITLINADPKF AQRKFDLPLD GSYVTIDPSV 

       130        140        150        160        170        180 
SDWSNYFKCG LHVAHSFLKK LAPERFASAP LAGLQVFCEG DVPTGSGLSS SAAFICAVAL 

       190        200        210        220        230        240 
AVVKANMGPG YHMSKQNLMR ITVVAEHYVG VNNGGMDQAA SVCGEEDHAL YVEFKPQLKA 

       250        260        270        280        290        300 
TPFKFPQLKN HEISFVIANT LVVSNKFETA PTNYNLRVVE VTTAANVLAA TYGVVLLSGK 

       310        320        330        340        350        360 
EGSSTNKGNL RDFMNVYYAR YHNISTPWNG DIESGIERLT KMLVLVEESL ANKKQGFSVD 

       370        380        390        400        410        420 
DVAQSLNCSR EEFTRDYLTT SPVRFQVLKL YQRAKHVYSE SLRVLKAVKL MTTASFTADE 

       430        440        450        460        470        480 
DFFKQFGALM NESQASCDKL YECSCPEIDK ICSIALSNGS YGSRLTGAGW GGCTVHLVPG 

       490        500        510        520 
GPNGNIEKVK EALANEFYKV KYPKITDAEL ENAIIVSKPA LGSCLYEL 

P04385 in FASTA format

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