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UniProtKB/Swiss-Prot entry P04175

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NCPR_PIG
Primary accession number P04175
Secondary accession numbers None
Integrated into Swiss-Prot on March 20, 1987
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 77)
Name and origin of the protein
Protein name NADPH--cytochrome P450 reductase
Synonyms CPR
P450R
EC 1.6.2.4
Gene name
Name: POR
From
Sus scrofa (Pig) [TaxID: 9823] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae; Sus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
Amborn J., Preiss B., Stender B., Viale M., Repp R.Z., Lampert F., Kroger M., Lumper L.;
Submitted (JUN-1995) to the EMBL/GenBank/DDBJ databases.
[2]
 PROTEIN SEQUENCE OF 2-678, AND ACETYLATION AT GLY-2.
DOI=10.1021/bi00372a018; PubMed=3099837 [NCBI, ExPASy, EBI, Israel, Japan]
Haniu M., Iyanagi T., Miller P., Lee T.D., Shively J.E.;
"Complete amino acid sequence of NADPH-cytochrome P-450 reductase from porcine hepatic microsomes.";
Biochemistry 25:7906-7911(1986).
[3]
 PROTEIN SEQUENCE OF 57-678.
PubMed=3098240 [NCBI, ExPASy, EBI, Israel, Japan]
Vogel F., Lumper L.;
"Complete structure of the hydrophilic domain in the porcine NADPH-cytochrome P-450 reductase.";
Biochem. J. 236:871-878(1986).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L33893; AAA85368.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A25584; RDPGO4.
RefSeq NP_001123431.1; -.
3D structure databases
HSSP P16435; 1B1C. [HSSP ENTRY / PDB]
SMR P04175; 64-678.
ModBase P04175.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0003958; Molecular function: NADPH-hemoprotein reductase activity (inferred from electronic annotation from EC).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR003097; FAD-binding_1.
IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR001709; FPN_cyt_redctse.
IPR015702; NADPH_Cyt_Red.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.
PANTHER PTHR19384:SF17; NADPH_Cyt_Red; 1.
Pfam PF00667; FAD_binding_1; 1.
PF00258; Flavodoxin_1; 1.
PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.
PRINTS PR00369; FLAVODOXIN.
PR00371; FPNCR.
PROSITE PS51384; FAD_FR; 1.
PS50902; FLAVODOXIN_LIKE; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P04175.
ProtoNet P04175.
Genome annotation databases
GeneID 100170114; -.
Phylogenomic databases
HOVERGEN P04175; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Direct protein sequencing; Endoplasmic reticulum; FAD; Flavoprotein; FMN; Membrane; NADP; Oxidoreductase; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   678  677     NADPH--cytochrome P450 reductase. PRO_0000167598
DOMAIN   80   224  145     Flavodoxin-like. 
DOMAIN   279   521  243     FAD-binding FR-type. 
NP_BIND   170   201  32     FMN (By similarity). 
NP_BIND   314   325  12     FAD (By similarity). 
NP_BIND   451   461  11     FAD (By similarity). 
NP_BIND   529   547  19     NADP (By similarity). 
NP_BIND   624   640  17     NADP (By similarity). 
MOD_RES   2     2        N-acetylglycine. 
MOD_RES   575   575        Phosphotyrosine (By similarity). 
CONFLICT   55    55        S -> T (in Ref. 2; AAA85368). 
CONFLICT   164   164        T -> S (in Ref. 2; AAA85368). 
CONFLICT   175   175        N -> D (in Ref. 3; AA sequence). 
CONFLICT   340   340        T -> A (in Ref. 3; AA sequence). 
CONFLICT   379   379        N -> D (in Ref. 3; AA sequence). 
CONFLICT   401   401        Q -> E (in Ref. 3; AA sequence). 
CONFLICT   447   447        R -> L (in Ref. 2; AAA85368). 
CONFLICT   503   503        N -> D (in Ref. 3; AA sequence). 
CONFLICT   509   509        V -> L (in Ref. 2 and 3). 
CONFLICT   675   675        D -> N (in Ref. 3; AA sequence). 
Sequence information
Length: 678 AA [This is the length of the unprocessed precursor] Molecular weight: 76833 Da [This is the MW of the unprocessed precursor] CRC64: 206079E13CA6E0A8 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MGDSNVDTGT TTSEMVAEEV SLFSATDMVL FSLIVGLLTY WFIFRKKKDE VPEFSKIETT 

        70         80         90        100        110        120 
TSSVKDSSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM AADPEEYDLS 

       130        140        150        160        170        180 
DLSSLPEIEN ALAVFCMATY GEGDPTDNAQ DFYDWLQEAD VDLTGVKYAV FGLGNKTYEH 

       190        200        210        220        230        240 
FNAMGKYVDK RLEQLGAQRI FDLGLGDDDG NLEEDFITWR EQFWPAVCEH FGVEATGEES 

       250        260        270        280        290        300 
SIRQYELVVH TDMDTAVVYT GEMGRLKSYE NQKPPFDAKN PFLAVVTTNR KLNQGTERHL 

       310        320        330        340        350        360 
MHLELDISDS KIRYESGDHV AVYPANDSAL VNQLGEILGT DLDIVMSLNN LDEESNKRHP 

       370        380        390        400        410        420 
FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE QLRKMASSSG EGKELYLSWV 

       430        440        450        460        470        480 
VEARRHILAI LQDYPSLRPP IDHLCERLPR LQARYYSIAS SSKVHPNSVH ICAVVVEYET 

       490        500        510        520        530        540 
KSGRVNKGVA TSWLRAKEPA GENGRRALVP MFVRKSQFRL PFKATTPVIM VGPGTGVAPF 

       550        560        570        580        590        600 
IGFIQERAWL QEQGKEVGET LLYYGCRRSD EDYLYREELA QFHAKGALTR LSVAFSREQP 

       610        620        630        640        650        660 
QKVYVQHLLK RDKEHLWKLI HDGGAHIYIC GDARNMARDV QNTFCDIVAE QGPMEHAQAV 

       670 
DYVKKLMTKG RYSLDVWS
P04175 in FASTA format

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