[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-147 (ISOFORM 4). Amgen EST program; "Amgen rat EST program."; Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [MRNA] OF 19-218 (ISOFORMS 1; 2; 3 AND 4). PubMed=2440674 [NCBI, ExPASy, EBI, Israel, Japan] Barclay A.N., Jackson D.I., Willis A.C., Williams A.F.; "Lymphocyte specific heterogeneity in the rat leucocyte common antigen (T200) is due to differences in polypeptide sequences near the NH2-terminus."; EMBO J. 6:1259-1264(1987).
[3]	NUCLEOTIDE SEQUENCE [MRNA] OF 19-1273 (ISOFORMS 1; 3; 4 AND 5). Barclay A.N., Jackson D.I., Willis A.C., Williams A.F.; Submitted (MAY-1987) to the EMBL/GenBank/DDBJ databases.
[4]	NUCLEOTIDE SEQUENCE [MRNA] OF 208-1255 (ISOFORM 1). DOI=10.1016/0092-8674(85)90063-7; PubMed=3158393 [NCBI, ExPASy, EBI, Israel, Japan] Thomas M.L., Barclay A.N., Gagnon J., Williams A.F.; "Evidence from cDNA clones that the rat leukocyte-common antigen (T200) spans the lipid bilayer and contains a cytoplasmic domain of 80,000 Mr."; Cell 41:83-93(1985).

Comments

FUNCTION: Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN.
CATALYTIC ACTIVITY: Protein tyrosine phosphate + H₂O = protein tyrosine + phosphate.
SUBUNIT: Interacts with SKAP1. Binds GANAB and PRKCSH (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.

ALTERNATIVE PRODUCTS: 5 named isoforms [FASTA] produced by alternative splicing. Additional isoforms seem to exist.

Name	1
Synonyms	L-CA variant 4
Isoform ID	P04157-1
This is the isoform sequence displayed in this entry.

Name	2
Synonyms	L-CA variant 1
Isoform ID	P04157-2
Features which should be applied to build the isoform sequence: VSP_005167.

Name	3
Synonyms	L-CA variant 2
Isoform ID	P04157-3
Features which should be applied to build the isoform sequence: VSP_005166.

Name	4
Synonyms	L-CA variant 3
Isoform ID	P04157-4
Features which should be applied to build the isoform sequence: VSP_005165, VSP_005168.

Name	5
Isoform ID	P04157-5
Features which should be applied to build the isoform sequence: VSP_026163.

TISSUE SPECIFICITY: Variants 4 and 3 are found in the lymph node, variants 1 and 2 are found in thymocyte and lymph node.
DOMAIN: The first PTPase domain interacts with SKAP1 (By similarity).
PTM: Heavily N- and O-glycosylated.
PTM: The cytoplasmic domain contains potential phosphorylation sites.
SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily.
SIMILARITY: Contains 2 fibronectin type-III domains.
SIMILARITY: Contains 2 tyrosine-protein phosphatase domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CB793707; -; NOT_ANNOTATED_CDS; mRNA.	[EMBL / GenBank / DDBJ]
Y00065; CAA68272.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Y00065; CAA68273.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Y00065; CAA68274.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Y00065; CAA68275.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M25820; AAA41518.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M25821; AAA41519.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M25822; AAA41520.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M25823; AAA41521.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A45854; A45854.

RefSeq

NP_001103357.1; -.
NP_001103358.1; -.
NP_001103359.1; -.
NP_001103360.1; -.
NP_612516.2; -.

UniGene

Rn.90166

3D structure databases

HSSP

P18052; 1YFO. [HSSP ENTRY / PDB]

P04157; 576-1183.

PTM databases

P04157; -.

Organism-specific databases

RGD

3451; Ptprc.

Gene expression databases

ArrayExpress

P04157; -.

GermOnline

ENSRNOG00000000655; Rattus norvegicus.

Ontologies

GO:0005925;	Cellular component: focal adhesion (inferred from sequence or structural similarity from UniProtKB).
GO:0005887;	Cellular component: integral to plasma membrane (inferred from direct assay from UniProtKB).
GO:0019901;	Molecular function: protein kinase binding (inferred from sequence or structural similarity from UniProtKB).
GO:0004725;	Molecular function: protein tyrosine phosphatase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0042100;	Biological process: B cell proliferation (inferred from sequence or structural similarity from UniProtKB).
GO:0050853;	Biological process: B cell receptor signaling pathway (inferred from sequence or structural similarity from UniProtKB).
GO:0051607;	Biological process: defense response to virus (inferred from sequence or structural similarity from UniProtKB).
GO:0001960;	Biological process: negative regulation of cytokine mediated signaling pathway (inferred from sequence or structural similarity from UniProtKB).
GO:0006469;	Biological process: negative regulation of protein kinase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0001915;	Biological process: negative regulation of T cell mediated cytotoxicity (inferred from sequence or structural similarity from UniProtKB).
GO:0050857;	Biological process: positive regulation of antigen receptor-mediated signaling pathway (inferred from sequence or structural similarity from UniProtKB).
GO:0042102;	Biological process: positive regulation of T cell proliferation (inferred from sequence or structural similarity from UniProtKB).
GO:0006470;	Biological process: protein amino acid dephosphorylation (inferred from sequence or structural similarity from UniProtKB).
GO:0051726;	Biological process: regulation of cell cycle (inferred from sequence or structural similarity from UniProtKB).
GO:0051209;	Biological process: release of sequestered calcium ion into cytosol (inferred from sequence or structural similarity from UniProtKB).
GO:0030217;	Biological process: T cell differentiation (inferred from sequence or structural similarity from UniProtKB).
GO:0050852;	Biological process: T cell receptor signaling pathway (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR008957; Fibronectin_typ-III-like_fold.
IPR003961; FN_III.
IPR000387; Tyr_Pase.
IPR016130; Tyr_Pase_AS.
IPR000242; Tyr_Pase_rcpt/non-rcpt.
Graphical view of domain structure.

Gene3D

G3DSA:2.60.40.30; FN_III-like; 2.

Pfam

PF00041; fn3; 2.
PF00102; Y_phosphatase; 2.
Pfam graphical view of domain structure.

PRINTS

PR00700; PRTYPHPHTASE.

SMART

SM00060; FN3; 2.
SM00194; PTPc; 2.
SMART graphical view of domain structure.

PROSITE

PS50853; FN3; 2.
PS00383; TYR_PHOSPHATASE_1; 1.
PS50056; TYR_PHOSPHATASE_2; 2.
PS50055; TYR_PHOSPHATASE_PTP; 2.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSRNOG00000000655; Rattus norvegicus. [Contig view]

GeneID

24699; -.

KEGG

rno:24699; -.

Phylogenomic databases

HOVERGEN

P04157; -.

Other

NextBio

604155; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Glycoprotein; Hydrolase; Membrane; Phosphoprotein; Protein phosphatase; Repeat; Signal; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 23`	`23`
`CHAIN`	`24 1273`	`1250`	`Leukocyte common antigen.`	`PRO_0000025472`
`TOPO_DOM`	`24 546`	`523`	`Extracellular (Potential).`
`TRANSMEM`	`547 567`	`21`	`Potential.`
`TOPO_DOM`	`568 1273`	`706`	`Cytoplasmic (Potential).`
`DOMAIN`	`361 449`	`89`	`Fibronectin type-III 1.`
`DOMAIN`	`454 541`	`88`	`Fibronectin type-III 2.`
`DOMAIN`	`622 881`	`260`	`Tyrosine-protein phosphatase 1.`
`DOMAIN`	`913 1196`	`284`	`Tyrosine-protein phosphatase 2.`
`ACT_SITE`	`822 822`		`Phosphocysteine intermediate (By similarity).`
`ACT_SITE`	`1137 1137`		`Phosphocysteine intermediate (By similarity).`
`MOD_RES`	`966 966`		`Phosphoserine (By similarity).`
`MOD_RES`	`974 974`		`Phosphoserine (By similarity).`
`MOD_RES`	`978 978`		`Phosphoserine (By similarity).`
`CARBOHYD`	`62 62`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`142 142`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`153 153`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`164 164`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`178 178`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`200 200`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`245 245`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`250 250`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`271 271`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`282 282`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`327 327`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`333 333`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`371 371`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`374 374`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`471 471`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`502 502`		`N-linked (GlcNAc...) (Potential).`
`VAR_SEQ`	`30 161`		`Missing (in isoform 2).`	`VSP_005167`
`VAR_SEQ`	`30 120`		`Missing (in isoform 3).`	`VSP_005166`
`VAR_SEQ`	`30 71`		`Missing (in isoform 4).`	`VSP_005165`
`VAR_SEQ`	`72 161`		`Missing (in isoform 5).`	`VSP_026163`
`VAR_SEQ`	`121 161`		`Missing (in isoform 4).`	`VSP_005168`
`CONFLICT`	`56 56`		`S -> R (in Ref. 3; AAA41518/AAA41521).`

Sequence information

Length: 1273 AA [This is the length of the unprocessed precursor]

Molecular weight: 143269 Da [This is the MW of the unprocessed precursor]

CRC64: EDFA48100ACF2CB2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MYLWLKLLAF SLALLGPEVF VTGQGTTDDG LDTTEIVLLP QTDPLPARTT EFTPPSISER 

        70         80         90        100        110        120 
GNGSSETTYL PGFSSTLMPH LTPQPDSQTP SARGADTQTL SSQADLTTLT AAPSGETDPP 

       130        140        150        160        170        180 
GVPEESTVPE TFPGGTPILA RNSTAPSPTH TSNVSTTDIS SGANLTTPAP STLGFASNTT 

       190        200        210        220        230        240 
TSTEIATPQT KPSCDEKFGN VTVRYIYDDS SKNFNANLEG DKKPKCEYTD CEKELKNLPE 

       250        260        270        280        290        300 
CSQKNVTLSN GSCTPDKIIN LDVPPGTHNF NLTNCTPDIE ANTSICLEWK IKNKFTCDIQ 

       310        320        330        340        350        360 
KISYNFRCTP EMKTFALDKH GTLWLHNLTV RTNYTCAAEV LYNNVILLKQ DRRVQTDFGT 

       370        380        390        400        410        420 
PEMLPHVQCK NSTNSTTLVS WAEPASKHHG YILCYKKTPS EKCENLANDV NSFEVKNLRP 

       430        440        450        460        470        480 
YTEYTVSLFA YVIGRVQRNG PAKDCNFRTK AARPGKVNGM KTSRASDNSI NVTCNSPYEI 

       490        500        510        520        530        540 
NGPEARYILE VKSGGSLVKT FNQSTCKFVV DNLYYSTDYE FLVYFYNGEY LGDPEIKPQS 

       550        560        570        580        590        600 
TSYNSKALII FLVFLIIVTS IALLVVLYKI YDLRKKRSSN LDEQQELVER DEEKQLINVD 

       610        620        630        640        650        660 
PIHSDLLLET YKRKIADEGR LFLAEFQSIP RVFSKFPIKD ARKSQNQNKN RYVDILPYDY 

       670        680        690        700        710        720 
NRVELSEING DAGSTYINAS YIDGFKEPRK YIAAQGPRDE TVDDFWKMIW EQKATVIVMV 

       730        740        750        760        770        780 
TRCEEGNRNK CAEYWPCMEE GTRTFRDVVV TINDHKRCPD YIIQKLSIAH KKEKATGREV 

       790        800        810        820        830        840 
THIQFTSWPD HGVPEDPHLL LKLRRRVNAF SNFFSGPIVV HCSAGVGRTG TYIGIDAMLE 

       850        860        870        880        890        900 
SLEAEGKVDV YGYVVNLRRQ RCLMVQVEAQ YILIHQALVE YNQFGETEVN LSELHSCLQN 

       910        920        930        940        950        960 
LKKRDPPSDP SPLEAEYQRL PSYRSWRTQH IGNQEENKKK NRSSNVVPYD FNRVPLKHEL 

       970        980        990       1000       1010       1020 
EMSKESEAES DESSDEDSDS EETSKYINAS FVMSYWKPEM MIAAQGPLKE TIGDFWQMIF 

      1030       1040       1050       1060       1070       1080 
QRKVKVIVML TELMSGDQEV CAQYWGEGKQ TYGDMEVMLK DTNKSSAYIL RAFELRHSKR 

      1090       1100       1110       1120       1130       1140 
KEPRTVYQYQ CTTWKGEELP AEPKDLVTLI QNIKQKLPKS GSEGMKYHKH ASILVHCRDG 

      1150       1160       1170       1180       1190       1200 
SQQTGLFCAL FNLLESAETE DVVDVFQVVK SLRKARPGMV GSFEQYQFLY DIMASIYPTQ 

      1210       1220       1230       1240       1250       1260 
NGQVKKANSQ DKIEFHNEVD GAKQDANCVQ PADPLNKAQE DSKEVGASEP ASGSEEPEHS 

      1270 
ANGPMSPALT PSS

P04157 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools