ID   LDH1_LACLC              Reviewed;         325 AA.
AC   P04034; P94885; P94886;
DT   01-NOV-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   04-NOV-2008, entry version 73.
DE   RecName: Full=L-lactate dehydrogenase 1;
DE            Short=L-LDH 1;
DE            EC=1.1.1.27;
GN   Name=ldhA; Synonyms=ldh;
OS   Lactococcus lactis subsp. cremoris (Streptococcus cremoris).
OC   Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus.
OX   NCBI_TaxID=1359;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ML1;
RX   MEDLINE=94362896; PubMed=8081494;
RA   Swindell S.R., Griffin H.G., Gasson M.J.;
RT   "Cloning, sequencing and comparison of three lactococcal L-lactate
RT   dehydrogenase genes.";
RL   Microbiology 140:1301-1305(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=AM4, AM5, CM1-3, and MSUA2;
RX   MEDLINE=97176401; PubMed=9023947;
RA   Urbach E., Daniels B., Salama M.S., Sandine W.E., Giovannoni S.J.;
RT   "The ldh phylogeny for environmental isolates of Lactococcus lactis is
RT   consistent with rRNA genotypes but not with phenotypes.";
RL   Appl. Environ. Microbiol. 63:694-702(1997).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-22 AND 247-298.
RC   STRAIN=US3;
RX   MEDLINE=80088398; PubMed=518918; DOI=10.1016/0005-2795(79)90254-X;
RA   Crossley L.G., Jago G.R., Davidson B.E.;
RT   "Partial sequence data for the L-(+)-lactate dehydrogenase from
RT   Streptococcus cremoris US3 including the amino acid sequences around
RT   the single cysteine residue and at the N-terminus.";
RL   Biochim. Biophys. Acta 581:342-355(1979).
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; U02385; AAA61961.1; -; Genomic_DNA.
DR   EMBL; U78630; AAB51674.1; -; Genomic_DNA.
DR   EMBL; U78631; AAB51675.1; -; Genomic_DNA.
DR   EMBL; U78633; AAB51677.1; -; Genomic_DNA.
DR   EMBL; U78635; AAB51679.1; -; Genomic_DNA.
DR   PIR; A20629; A20629.
DR   HSSP; P00343; 1LLC.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00488; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DHase.
DR   InterPro; IPR011304; L-lactate_DHase.
DR   InterPro; IPR001236; Lactate/malate_DHase.
DR   InterPro; IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase;
KW   Phosphoprotein.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    325       L-lactate dehydrogenase 1.
FT                                /FTId=PRO_0000168354.
FT   NP_BIND      15     43       NAD (By similarity).
FT   ACT_SITE    178    178       Proton acceptor (By similarity).
FT   BINDING      91     91       Substrate (By similarity).
FT   BINDING     123    123       NAD or substrate (By similarity).
FT   BINDING     154    154       Substrate (By similarity).
FT   BINDING     232    232       Substrate (By similarity).
FT   MOD_RES     223    223       Phosphotyrosine (By similarity).
FT   VARIANT     127    127       I -> T (in strain: CM1-3).
FT   VARIANT     213    213       K -> E (in strain: AM4 and CM1-3).
FT   VARIANT     293    293       D -> E (in strain: AM4).
FT   CONFLICT      5      5       Q -> E (in Ref. 3; AA sequence).
FT   CONFLICT     20     20       S -> A (in Ref. 3; AA sequence).
SQ   SEQUENCE   325 AA;  35050 MW;  6F50D8343AF13AA9 CRC64;
     MADKQRKKVI LVGDGAVGSS YAFALVNQGI AQELGIVDLF KEKTQGDAED LSHALAFTSP
     KKIYSADYSD ASDADLVVLT SGAPQKPGET RLDLVEKNLR ITKDVVTKIV ASGFKGIFLV
     AANPVDILTY ATWKFSGFPK NRVVGSGTSL DTARFRQALA EKVDVDARSI HAYIMGEHGD
     SEFAVWSHAN VAGVKLEQWF QENDYLNEAE IVKLFESVRD AAYSIIAKKG ATFYGVAVAL
     ARITKAILDD EHAVLPVSVF QDGQYGVSDC YLGQPAVVGA EGVVNPIHIP LNDAEMQKME
     ASGAQLKAII DEAFAKEEFA SAVKN
//