ID   ORF2A_BMV               Reviewed;         822 AA.
AC   P03594;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   04-NOV-2008, entry version 47.
DE   RecName: Full=RNA-directed RNA polymerase 2A;
DE            Short=protein 2A;
DE            EC=2.7.7.48;
GN   Name=RNA2; ORFNames=ORF2a;
OS   Brome mosaic virus (BMV).
OC   Viruses; ssRNA positive-strand viruses, no DNA stage; Bromoviridae;
OC   Bromovirus.
OX   NCBI_TaxID=12302;
OH   NCBI_TaxID=15371; Bromus inermis (Smooth brome grass).
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   MEDLINE=84114904; PubMed=6694215; DOI=10.1016/S0022-2836(84)80012-1;
RA   Ahlquist P., Dasgupta R., Kaesberg P.;
RT   "Nucleotide sequence of the brome mosaic virus genome and its
RT   implications for viral replication.";
RL   J. Mol. Biol. 172:369-383(1984).
CC   -!- FUNCTION: RNA-directed RNA polymerase.
CC   -!- CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate
CC       + RNA(n+1).
CC   -!- MISCELLANEOUS: The virus has a tripartite genome (RNA1, RNA2 and
CC       RNA3) and an encapsidated subgenomic RNA (RNA4) from which the
CC       coat protein is expressed. Virions are icosahedral, not enveloped,
CC       with 32 capsomers per nucleocapsid.
CC   -!- SIMILARITY: Belongs to the cucumoviruses/bromoviruses 2A family.
CC   -!- SIMILARITY: Contains 1 RdRp catalytic domain.
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DR   EMBL; X01678; CAA25834.1; -; Unassigned_RNA.
DR   PIR; A04202; P2BVA.
DR   RefSeq; NP_041197.1; -.
DR   GeneID; 962145; -.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0006410; P:transcription, RNA-dependent; IEA:UniProtKB-KW.
DR   InterPro; IPR007610; DUF585.
DR   InterPro; IPR001788; RNA-dep_RNA_pol_vir-typ.
DR   InterPro; IPR007094; RNA_pol_PSvir.
DR   Pfam; PF04522; DUF585; 1.
DR   Pfam; PF00978; RdRP_2; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; RNA replication;
KW   RNA-directed RNA polymerase; Transferase.
FT   CHAIN         1    822       RNA-directed RNA polymerase 2A.
FT                                /FTId=PRO_0000083269.
FT   DOMAIN      459    572       RdRp catalytic.
SQ   SEQUENCE   822 AA;  93927 MW;  C13B99016B2C54AC CRC64;
     MSSKTWDDDF VRQVPSFQWI IDQSLEDEVE AASLQVQEPA DGVAIDGSLA SFKLAIAPLE
     IGGVFDPPFD RVRWGSICDT VQQMVQQFTD RPLIPQAEMA RMLYLDIPGS FVLEDEIDDW
     YPEDTSDGYG VSFAADEDHA SDLKLASDSS NCEIEEVRVT GDTPKELTLG DRYMGIDEEF
     QTTNTDYDIT LQIMNPIEHR VSRVIDTHCH PDNPDISTGP IYMERVSLAR TEATSHSILP
     THAYFDDSYH QALVENGDYS MDFDRIRLKQ SDVDWYRDPD KYFQPKMNIG SAQRRVGTQK
     EVLTALKKRN ADVPEMGDAI NMKDTAKAIA KRFRSTFLNV DGEDCLRASM DVMTKCLEYH
     KKWGKHMDLQ GVNVAAETDL CRYQHMLKSD VKPVVTDTLH LERAVAATIT FHSKGVTSNF
     SPFFTACFEK LSLALKSRFI VPIGKISSLE LKNVRLNNRY FLEADLSKFD KSQGELHLEF
     QREILLALGF PAPLTNWWSD FHRDSYLSDP HAKVGMSVSF QRRTGDAFTY FGNTLVTMAM
     IAYASDLSDC DCAIFSGDDS LIISKVKPVL DTDMFTSLFN MEIKVMDPSV PYVCSKFLVE
     TEMGNLVSVP DPLREIQRLA KRKILRDEQM LRAHFVSFCD RMKFINQLDE KMITTLCHFV
     YLKYGKEKPW IFEEVRAALA AFSLYSENFL RFSDCYCTEG IRVYQMSDPV CKFKRTTEER
     KTDGDWFHNW KNPKFPGVLD KVYRTIGIYS SDCSTKELPV KRIGRLHEAL ERESLKLAND
     RRTTQRLKKK VDDYATGRGG LTSVDALLVK SHCETFKPSD LR
//