[1]	NUCLEOTIDE SEQUENCE. DOI=10.1016/S0022-2836(84)80012-1; PubMed=6694215 [NCBI, ExPASy, EBI, Israel, Japan] Ahlquist P., Dasgupta R., Kaesberg P.; "Nucleotide sequence of the brome mosaic virus genome and its implications for viral replication."; J. Mol. Biol. 172:369-383(1984).

Comments

FUNCTION: RNA-directed RNA polymerase.
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
MISCELLANEOUS: The virus has a tripartite genome (RNA1, RNA2 and RNA3) and an encapsidated subgenomic RNA (RNA4) from which the coat protein is expressed. Virions are icosahedral, not enveloped, with 32 capsomers per nucleocapsid.
SIMILARITY: Belongs to the cucumoviruses/bromoviruses 2A family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X01678; CAA25834.1; -; Unassigned_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A04202; P2BVA.

RefSeq

NP_041197.1; -.

3D structure databases

ModBase

P03594.

Family and domain databases

InterPro

IPR007610; DUF585.
IPR001788; RNA-dep_RNA_pol_vir-typ.
IPR007094; RNA_pol_PSvir.
Graphical view of domain structure.

Pfam

PF04522; DUF585; 1.
PF00978; RdRP_2; 1.
Pfam graphical view of domain structure.

PROSITE

PS50507; RDRP_SSRNA_POS; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

P03594.

Genome annotation databases

GeneID

962145; -.

Other

ProtoNet

P03594.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 822`	`822`	`RNA-directed RNA polymerase 2A.`	`PRO_0000083269`
`DOMAIN`	`459 572`	`114`	`RdRp catalytic.`

Sequence information

Length: 822 AA [This is the length of the unprocessed precursor]

Molecular weight: 93927 Da [This is the MW of the unprocessed precursor]

CRC64: C13B99016B2C54AC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSKTWDDDF VRQVPSFQWI IDQSLEDEVE AASLQVQEPA DGVAIDGSLA SFKLAIAPLE 

        70         80         90        100        110        120 
IGGVFDPPFD RVRWGSICDT VQQMVQQFTD RPLIPQAEMA RMLYLDIPGS FVLEDEIDDW 

       130        140        150        160        170        180 
YPEDTSDGYG VSFAADEDHA SDLKLASDSS NCEIEEVRVT GDTPKELTLG DRYMGIDEEF 

       190        200        210        220        230        240 
QTTNTDYDIT LQIMNPIEHR VSRVIDTHCH PDNPDISTGP IYMERVSLAR TEATSHSILP 

       250        260        270        280        290        300 
THAYFDDSYH QALVENGDYS MDFDRIRLKQ SDVDWYRDPD KYFQPKMNIG SAQRRVGTQK 

       310        320        330        340        350        360 
EVLTALKKRN ADVPEMGDAI NMKDTAKAIA KRFRSTFLNV DGEDCLRASM DVMTKCLEYH 

       370        380        390        400        410        420 
KKWGKHMDLQ GVNVAAETDL CRYQHMLKSD VKPVVTDTLH LERAVAATIT FHSKGVTSNF 

       430        440        450        460        470        480 
SPFFTACFEK LSLALKSRFI VPIGKISSLE LKNVRLNNRY FLEADLSKFD KSQGELHLEF 

       490        500        510        520        530        540 
QREILLALGF PAPLTNWWSD FHRDSYLSDP HAKVGMSVSF QRRTGDAFTY FGNTLVTMAM 

       550        560        570        580        590        600 
IAYASDLSDC DCAIFSGDDS LIISKVKPVL DTDMFTSLFN MEIKVMDPSV PYVCSKFLVE 

       610        620        630        640        650        660 
TEMGNLVSVP DPLREIQRLA KRKILRDEQM LRAHFVSFCD RMKFINQLDE KMITTLCHFV 

       670        680        690        700        710        720 
YLKYGKEKPW IFEEVRAALA AFSLYSENFL RFSDCYCTEG IRVYQMSDPV CKFKRTTEER 

       730        740        750        760        770        780 
KTDGDWFHNW KNPKFPGVLD KVYRTIGIYS SDCSTKELPV KRIGRLHEAL ERESLKLAND 

       790        800        810        820 
RRTTQRLKKK VDDYATGRGG LTSVDALLVK SHCETFKPSD LR

P03594 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools