[1]	NUCLEOTIDE SEQUENCE [MRNA]. PubMed=2833669 [NCBI, ExPASy, EBI, Israel, Japan] Stewart A.F., Bonsing J., Beattie C.W., Shah F., Willis I.M., Mackinlay A.G.; "Complete nucleotide sequences of bovine alpha S2- and beta-casein cDNAs: comparisons with related sequences in other species."; Mol. Biol. Evol. 4:231-241(1987).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Crossbred X Angus; TISSUE=Liver; NIH - Mammalian Gene Collection (MGC) project; Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
[3]	PROTEIN SEQUENCE OF 16-222 (A ALLELE). TISSUE=Milk; DOI=10.1016/0014-5793(77)80167-1; PubMed=862906 [NCBI, ExPASy, EBI, Israel, Japan] Brignon G., Ribadeau-Dumas B., Mercier J.-C., Pelissier J.-P., Das B.C.; "Complete amino acid sequence of bovine alphaS2-casein."; FEBS Lett. 76:274-279(1977).
[4]	PARTIAL PROTEIN SEQUENCE (D ALLELE). TISSUE=Milk; PubMed=469044 [NCBI, ExPASy, EBI, Israel, Japan] Grosclaude F., Joudrier P., Mahe M.-F.; "A genetic and biochemical analysis of a polymorphism of bovine alpha S2-casein."; J. Dairy Res. 46:211-213(1979).
[5]	PROTEIN SEQUENCE OF 165-203, CHARACTERIZATION OF CASOCIDIN, AND MASS SPECTROMETRY. TISSUE=Milk; DOI=10.1016/0014-5793(95)00974-E; PubMed=7556666 [NCBI, ExPASy, EBI, Israel, Japan] Zucht H.-D., Raida M., Adermann K., Meagert H.-J., Forssmann W.-G.; "Casocidin-I: a casein-alpha s2 derived peptide exhibits antibacterial activity."; FEBS Lett. 372:185-188(1995).
[6]	PHOSPHORYLATION AT SER-46 AND SER-158. Bai F., Liu S., Witzmann F.A.; Submitted (SEP-2005) to UniProtKB.
[7]	PHOSPHORYLATION AT SER-23; SER-24; SER-25; SER-28; SER-71; SER-72; SER-73; SER-76 AND SER-158, AND MASS SPECTROMETRY. DOI=10.1074/mcp.M600480-MCP200; PubMed=17510049 [NCBI, ExPASy, EBI, Israel, Japan] Imanishi S.Y., Kochin V., Ferraris S.E., de Thonel A., Pallari H.M., Corthals G.L., Eriksson J.E.; "Reference-facilitated phosphoproteomics: fast and reliable phosphopeptide validation by micro LC-ESI-Q-TOF MS/MS."; Mol. Cell. Proteomics 6:1380-1391(2007).

Comments

FUNCTION: Important role in the capacity of milk to transport calcium phosphate.
FUNCTION: Casocidin-I inhibits the growth of E.coli and S.carnosus.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Mammary gland specific. Secreted in milk.
MASS SPECTROMETRY: Mass=4870; Method=Electrospray; Range=165-203; Source=PubMed:7556666;.
POLYMORPHISM: At least two alleles exist. The sequence of the A allele is shown here. The D allele sequence differs from that shown in having a deletion of nine residues, which may be 49-58, 50-59, or 51-60.
SIMILARITY: Belongs to the alpha-casein family.
WEB RESOURCE: Name=Protein Spotlight; Note=Of buttons, digestion and glue - Issue 16 of November 2001; URL="http://www.expasy.org/spotlight/back_issues/sptlt016.shtml";.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M16644; AAA30479.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC114773; AAI14774.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

JQ2008; KABOS2.

NP_776953.1; -.

3D structure databases

ModBase

P02663.

Ontologies

GO:0042742;	Biological process: defense response to bacterium (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001588; Casein.
IPR011175; Casein_alpha-s2.
Graphical view of domain structure.

PANTHER

PTHR16656:SF2; Alpha-s2-casein; 1.

Pfam

PF00363; Casein; 2.
Pfam graphical view of domain structure.

PIRSF

PIRSF002371; Alpha-s2-casein; 1.

PROSITE

PS00306; CASEIN_ALPHA_BETA; 1.

Genome annotation databases

Ensembl

ENSBTAG00000005005; Bos taurus. [Contig view]

GeneID

282209; -.

KEGG

bta:282209; -.

Phylogenomic databases

HOVERGEN

P02663; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Antibiotic; Antimicrobial; Direct protein sequencing; Milk protein; Phosphoprotein; Repeat; Secreted; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 15`	`15`
`CHAIN`	`16 222`	`207`	`Alpha-S2-casein.`	`PRO_0000004460`
`PEPTIDE`	`165 203`	`39`	`Casocidin-1.`	`PRO_0000004461`
`REPEAT`	`76 140`	`65`
`REPEAT`	`158 222`	`65`
`MOD_RES`	`23 23`		`Phosphoserine.`
`MOD_RES`	`24 24`		`Phosphoserine.`
`MOD_RES`	`25 25`		`Phosphoserine.`
`MOD_RES`	`31 31`		`Phosphoserine.`
`MOD_RES`	`46 46`		`Phosphoserine.`
`MOD_RES`	`71 71`		`Phosphoserine.`
`MOD_RES`	`72 72`		`Phosphoserine.`
`MOD_RES`	`73 73`		`Phosphoserine.`
`MOD_RES`	`76 76`		`Phosphoserine.`
`MOD_RES`	`144 144`		`Phosphoserine.`
`MOD_RES`	`146 146`		`Phosphoserine.`
`MOD_RES`	`158 158`		`Phosphoserine.`
`CONFLICT`	`42 42`		`A -> D (in Ref. 2; AAI14774).`
`CONFLICT`	`102 102`		`Q -> E (in Ref. 3; AA sequence).`
`CONFLICT`	`105 105`		`Q -> R (in Ref. 2; AAI14774).`

Sequence information

Length: 222 AA [This is the length of the unprocessed precursor]

Molecular weight: 26019 Da [This is the MW of the unprocessed precursor]

CRC64: 81E7408AF1C12F7C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKFFIFTCLL AVALAKNTME HVSSSEESII SQETYKQEKN MAINPSKENL CSTFCKEVVR 

        70         80         90        100        110        120 
NANEEEYSIG SSSEESAEVA TEEVKITVDD KHYQKALNEI NQFYQKFPQY LQYLYQGPIV 

       130        140        150        160        170        180 
LNPWDQVKRN AVPITPTLNR EQLSTSEENS KKTVDMESTE VFTKKTKLTE EEKNRLNFLK 

       190        200        210        220 
KISQRYQKFA LPQYLKTVYQ HQKAMKPWIQ PKTKVIPYVR YL

P02663 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools