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UniProtKB/Swiss-Prot entry P01355


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CCKN_RAT
Primary accession number P01355
Secondary accession numbers None
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on July 21, 1986 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 65)
Name and origin of the protein
Protein name Cholecystokinin [Precursor]
Synonym CCK
Contains Cholecystokinin-39
     (CCK39)
Cholecystokinin-33
     (CCK33)
Cholecystokinin-22
     (CCK22)
Cholecystokinin-12
     (CCK12)
Cholecystokinin-8
     (CCK8)
Gene name
Name: Cck
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=6209267 [NCBI, ExPASy, EBI, Israel, Japan]
Kuwano R., Araki K., Usui H., Fukui T., Ohtsuka E., Ikehara M., Takahashi Y.;
"Molecular cloning and nucleotide sequence of cDNA coding for rat brain cholecystokinin precursor.";
J. Biochem. 96:923-926(1984).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=6199787 [NCBI, ExPASy, EBI, Israel, Japan]
Deschenes R.J., Lorenz L.J., Haun R.S., Roos B.A., Collier K.J., Dixon J.E.;
"Cloning and sequence analysis of a cDNA encoding rat preprocholecystokinin.";
Proc. Natl. Acad. Sci. U.S.A. 81:726-730(1984).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=3861130 [NCBI, ExPASy, EBI, Israel, Japan]
Deschenes R.J., Haun R.S., Sunkel D., Roos B.A., Dixon J.E.;
"Modulation of cholecystokinin gene expression.";
Ann. N. Y. Acad. Sci. 448:53-60(1985).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=2981840 [NCBI, ExPASy, EBI, Israel, Japan]
Deschenes R.J., Haun R.S., Funckes C.L., Dixon J.E.;
"A gene encoding rat cholecystokinin. Isolation, nucleotide sequence, and promoter activity.";
J. Biol. Chem. 260:1280-1286(1985).
[5]
SULFATION AT TYR-111 AND TYR-113.
Varro A., Young J., Gregory H., Csech J., Dockray G.J.;
"Isolation, structure and properties of the C-terminal fragment of the rat cholecystokinin precursor.";
Regul. Pept. 15:195-195(1986).
[6]
SULFATION AT TYR-97.
DOI=10.1016/0143-4179(94)90130-9; PubMed=8208365 [NCBI, ExPASy, EBI, Israel, Japan]
Beinfeld M.C.;
"Inhibition of pro-cholecystokinin (CCK) sulfation by treatment with sodium chlorate alters its processing and decreases cellular content and secretion of CCK 8.";
Neuropeptides 26:195-200(1994).
[7]
REVIEW.
DOI=10.1016/S0024-3205(02)02330-5; PubMed=12479974 [NCBI, ExPASy, EBI, Israel, Japan]
Beinfeld M.C.;
"Biosynthesis and processing of pro CCK: recent progress and future challenges.";
Life Sci. 72:747-757(2003).
Comments
  • FUNCTION: This peptide hormone induces gall bladder contraction and the release of pancreatic enzymes in the gut. Its function in the brain is not clear. Binding to CCK-A receptors stimulates amylase release from the pancreas, binding to CCK-B receptors stimulates gastric acid secretion.
  • SUBUNIT: Binds to CCK-A receptors in the pancreas and CCK-B receptors in the brain (By similarity).
  • SUBCELLULAR LOCATION: Secreted.
  • TISSUE SPECIFICITY: The shortest form (CCK8) is predominantly found in the brain, whereas the larger ones are found in the intestine.
  • PTM: The precursor is cleaved by proteases to produce a number of active cholecystokinins.
  • PTM: Sulfation of Tyr-97 is essential for receptor activation.
  • SIMILARITY: Belongs to the gastrin/cholecystokinin family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X01032; CAA25517.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K01259; AAA40897.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M25942; AAA40898.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M10353; AAB61086.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M10352; AAB61086.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A01624; GMRTCP.
RefSeq NP_036961.1; -.
UniGene Rn.9781
3D structure databases
ModBase P01355.
Organism-specific databases
RGD 2288; Cck.
Gene expression databases
ArrayExpress P01355; -.
GermOnline ENSRNOG00000019321; Rattus norvegicus.
Family and domain databases
InterPro IPR015499; CCK.
IPR001651; Gastrin.
IPR013152; Gastrin_CCK_CS.
Graphical view of domain structure.
PANTHER PTHR10786; CCK; 1.
Pfam PF00918; Gastrin; 1.
Pfam graphical view of domain structure.
SMART SM00029; GASTRIN; 1.
SMART graphical view of domain structure.
PROSITE PS00259; GASTRIN; 1.
BLOCKS P01355.
ProtoNet P01355.
Genome annotation databases
Ensembl ENSRNOG00000019321; Rattus norvegicus. [Contig view]
GeneID 25298; -.
KEGG rno:25298; -.
Phylogenomic databases
HOVERGEN P01355; -.
Other
NextBio 606067; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amidation; Cleavage on pair of basic residues; Hormone; Secreted; Signal; Sulfation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    20  20     By similarity. 
PROPEP   21    45  25      PRO_0000010565
CHAIN   46   103  58     Cholecystokinin. PRO_0000010566
PROPEP   47    63  17      PRO_0000010567
PEPTIDE   65   103  39     Cholecystokinin-39. PRO_0000010568
PEPTIDE   71   103  33     Cholecystokinin-33. PRO_0000010569
PEPTIDE   82   103  22     Cholecystokinin-22. PRO_0000010570
PEPTIDE   92   103  12     Cholecystokinin-12. PRO_0000010571
PEPTIDE   96   103  8     Cholecystokinin-8. PRO_0000010572
PROPEP   107   115  9      PRO_0000010573
SITE   106   107  2     Cleavage. 
MOD_RES   97    97        Sulfotyrosine. 
MOD_RES   103   103        Phenylalanine amide. 
MOD_RES   111   111        Sulfotyrosine. 
MOD_RES   113   113        Sulfotyrosine. 
Sequence information
Length: 115 AA [This is the length of the unprocessed precursor] Molecular weight: 12841 Da [This is the MW of the unprocessed precursor] CRC64: 0A713472CAD0F3DF [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKCGVCLCVV MAVLAAGALA QPVVPVEAVD PMEQRAEEAP RRQLRAVLRP DSEPRARLGA 

        70         80         90        100        110 
LLARYIQQVR KAPSGRMSVL KNLQGLDPSH RISDRDYMGW MDFGRRSAED YEYPS 

P01355 in FASTA format

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