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UniProtKB/Swiss-Prot entry P01317


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name INS_BOVIN
Primary accession number P01317
Secondary accession numbers None
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on August 1, 1992 (Sequence version 2)
Annotations were last modified on    June 10, 2008 (Entry version 97)
Name and origin of the protein
Protein name Insulin [Precursor]
Synonyms None
Contains Insulin B chain
Insulin A chain
Gene name
Name: INS
From
Bos taurus (Bovine) [TaxID: 9913] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=2456452 [NCBI, ExPASy, EBI, Israel, Japan]
D'Agostino J., Younes M.A., White J.W., Besch P.K., Field J.B., Frazier M.L.;
"Cloning and nucleotide sequence analysis of complementary deoxyribonucleic acid for bovine preproinsulin.";
Mol. Endocrinol. 1:327-331(1987).
[2]
PROTEIN SEQUENCE OF 25-105.
PubMed=4928892 [NCBI, ExPASy, EBI, Israel, Japan]
Nolan C., Margoliash E., Peterson J.D., Steiner D.F.;
"The structure of bovine proinsulin.";
J. Biol. Chem. 246:2780-2795(1971).
[3]
PROTEIN SEQUENCE OF 25-54.
PubMed=14886311 [NCBI, ExPASy, EBI, Israel, Japan]
Sanger F., Tuppy H.;
"The amino-acid sequence in the phenylalanyl chain of insulin. 2. The investigation of peptides from enzymic hydrolysates.";
Biochem. J. 49:481-490(1951).
[4]
PROTEIN SEQUENCE OF 57-82.
PubMed=5545080 [NCBI, ExPASy, EBI, Israel, Japan]
Steiner D.F., Cho S., Oyer P.E., Terris S., Peterson J.D., Rubenstein A.H.;
"Isolation and characterization of proinsulin C-peptide from bovine pancreas.";
J. Biol. Chem. 246:1365-1374(1971).
[5]
PROTEIN SEQUENCE OF 57-82.
PubMed=5105368 [NCBI, ExPASy, EBI, Israel, Japan]
Salokangas A., Smyth D.G., Markussen J., Sundby F.;
"Bovine proinsulin: amino acid sequence of the C-peptide isolated from pancreas.";
Eur. J. Biochem. 20:183-189(1971).
[6]
PROTEIN SEQUENCE OF 85-105.
PubMed=13032079 [NCBI, ExPASy, EBI, Israel, Japan]
Sanger F., Thompson E.O.P.;
"The amino-acid sequence in the glycyl chain of insulin. 2. The investigation of peptides from enzymic hydrolysates.";
Biochem. J. 53:366-374(1953).
[7]
PROTEIN SEQUENCE OF 25-54 AND 85-105, AND DISULFIDE BONDS.
PubMed=13249947 [NCBI, ExPASy, EBI, Israel, Japan]
Ryle A.P., Sanger F., Smith L.F., Kitai R.;
"The disulphide bonds of insulin.";
Biochem. J. 60:541-556(1955).
[8]
X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 26-54.
Smith G.D., Duax W.L., Dodson E.J., Dodson G.G., de Graaf R.A.G., Reynolds C.D.;
"The structure of des-Phe b1 bovine insulin.";
Acta Crystallogr. B 38:3028-3032(1982).
[9]
X-RAY CRYSTALLOGRAPHY (1.3 ANGSTROMS) OF 25-49.
DOI=10.1002/(SICI)1097-0134(199704)27:4<507::AID-PROT4>3.3.CO;2-H; PubMed=9141131 [NCBI, ExPASy, EBI, Israel, Japan]
Brange J., Dodson G.G., Edwards D.J., Holden P.H., Whittingham J.L.;
"A model of insulin fibrils derived from the X-ray crystal structure of a monomeric insulin (despentapeptide insulin).";
Proteins 27:507-516(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M54979; AAA30722.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A40909; IPBO.
RefSeq NP_776351.1; -.
UniGene Bt.453
3D structure databases
PDB
1APH; X-ray; 2.00 A; A=85-105, B=25-54.[ExPASy / RCSB / EBI]
1BPH; X-ray; 2.00 A; A=85-105, B=25-54.[ExPASy / RCSB / EBI]
1CPH; X-ray; 1.90 A; A=85-105, B=25-54.[ExPASy / RCSB / EBI]
1DPH; X-ray; 1.90 A; A=85-105, B=25-54.[ExPASy / RCSB / EBI]
1HO0; NMR; -; A=25-54.[ExPASy / RCSB / EBI]
1PID; X-ray; 1.30 A; A/C=85-105, B/D=25-49.[ExPASy / RCSB / EBI]
2A3G; X-ray; 2.25 A; A/C=85-105, B/D=25-54.[ExPASy / RCSB / EBI]
2BN1; X-ray; 1.40 A; A=85-105, B=25-54.[ExPASy / RCSB / EBI]
2BN3; X-ray; 1.40 A; A=85-105, B=25-54.[ExPASy / RCSB / EBI]
2INS; X-ray; 2.50 A; A/C=85-105, B/D=26-54.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1APH; -.
1BPH; -.
1CPH; -.
1DPH; -.
1HO0; -.
1PID; -.
2A3G; -.
2BN1; -.
2BN3; -.
2INS; -.
SMR P01317; 25-105.
ModBase P01317.
Family and domain databases
InterPro IPR004825; Ins/IGF/relaxin.
IPR003234; Insulin-related_peptide.
Graphical view of domain structure.
Gene3D G3DSA:1.10.100.10; Ins/IGF/relaxin; 1.
Pfam PF00049; Insulin; 1.
Pfam graphical view of domain structure.
PRINTS PR00277; INSULINB.
ProDom PD015667; Mollusc_ins; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00078; IlGF; 1.
SMART graphical view of domain structure.
PROSITE PS00262; INSULIN; 1.
BLOCKS P01317.
Genome annotation databases
Ensembl ENSBTAG00000013003; Bos taurus. [Contig view]
GeneID 280829; -.
KEGG bta:280829; -.
Phylogenomic databases
HOVERGEN P01317; -.
Other
LinkHub P01317; -.
ProtoNet P01317.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Carbohydrate metabolism; Cleavage on pair of basic residues; Direct protein sequencing; Glucose metabolism; Hormone; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom  To Length Description FTId
SIGNAL   1    24  24      
PEPTIDE   25    54  30     Insulin B chain. PRO_0000015764
PROPEP   57    82  26     C peptide. PRO_0000015765
PEPTIDE   85   105  21     Insulin A chain. PRO_0000015766
DISULFID   31    91        Interchain (between B and A chains). 
DISULFID   43   104        Interchain (between B and A chains). 
DISULFID   90    95         
HELIX   32    43  12      
HELIX   44    46  3      
HELIX   86    90  5      
HELIX   97   101  5      
Sequence information
Length: 105 AA [This is the length of the unprocessed precursor] Molecular weight: 11393 Da [This is the MW of the unprocessed precursor] CRC64: 75307CF78E61C06A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MALWTRLRPL LALLALWPPP PARAFVNQHL CGSHLVEALY LVCGERGFFY TPKARREVEG 

        70         80         90        100 
PQVGALELAG GPGAGGLEGP PQKRGIVEQC CASVCSLYQL ENYCN 

P01317 in FASTA format

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View entry in raw text format (no links)
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BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

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