ID INS_PIG Reviewed; 108 AA. AC P01315; Q9TSJ5; DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot. DT 01-DEC-2000, sequence version 2. DT 02-SEP-2008, entry version 96. DE RecName: Full=Insulin; DE Contains: DE RecName: Full=Insulin B chain; DE Contains: DE RecName: Full=Insulin A chain; DE Flags: Precursor; GN Name=INS; OS Sus scrofa (Pig). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae; OC Sus. OX NCBI_TaxID=9823; RN [1] RP NUCLEOTIDE SEQUENCE. RA Han X.G., Tuch B.E.; RT "Complete porcine preproinsulin cDNA sequence."; RL Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases. RN [2] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=Large white; RX MEDLINE=22135958; PubMed=12140686; DOI=10.1007/s00335-001-3059-x; RA Amarger V., Nguyen M., Van Laere A.-S., Braunschweig M., Nezer C., RA Georges M., Andersson L.; RT "Comparative sequence analysis of the INS-IGF2-H19 gene cluster in RT pigs."; RL Mamm. Genome 13:388-398(2002). RN [3] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC STRAIN=European wild boar, Hampshire, Japanese wild boar, Landrace, RC Large white, Meishan, and Pietrain; RX MEDLINE=22935770; PubMed=14574411; DOI=10.1038/nature02064; RA Van Laere A.-S., Nguyen M., Braunschweig M., Nezer C., Collette C., RA Moreau L., Archibald A.L., Haley C., Buys N., Tally M., Andersson G., RA Georges M., Andersson L.; RT "A regulatory mutation in IGF2 causes a major QTL effect on muscle RT growth in the pig."; RL Nature 425:832-836(2003). RN [4] RP PROTEIN SEQUENCE OF 25-108. RX MEDLINE=68286485; PubMed=5657063; RA Chance R.E., Ellis R.M., Bromer W.W.; RT "Porcine proinsulin: characterization and amino acid sequence."; RL Science 161:165-167(1968). RN [5] RP SEQUENCE REVISION TO 59. RA Chance R.E.; RL Submitted (JUL-1970) to the PIR data bank. RN [6] RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS). RA Blundell T.L., Dodson G.G., Hodgkin D., Mercola D.; RT "Insulin. The structure in the crystal and its reflection in chemistry RT and biology."; RL Adv. Protein Chem. 26:279-402(1972). RN [7] RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS). RA Isaacs N.W., Agarwal R.C.; RT "Experience with fast Fourier least squares in the refinement of the RT crystal structure of rhombohedral 2-zinc insulin at 1.5-A RT resolution."; RL Acta Crystallogr. A 34:782-791(1978). RN [8] RP X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS). RX MEDLINE=89099318; PubMed=2905485; RA Baker E.N., Blundell T.L., Cutfield J.F., Cutfield S.M., Dodson E.J., RA Dodson G.G., Crowfoot Hodgkin D.M., Hubbard R.E., Isaacs N.W., RA Reynolds C.D., Sakabe K., Sakabe N., Vijayan N.M.; RT "The structure of 2Zn pig insulin crystals at 1.5-A resolution."; RL Philos. Trans. R. Soc. Lond., B, Biol. Sci. 319:369-456(1988). RN [9] RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS). RX MEDLINE=92126280; PubMed=1772633; DOI=10.1107/S010876819100842X; RA Balschmidt P., Hansen F.B., Dodson E., Dodson G., Korber F.; RT "Structure of porcine insulin cocrystallized with clupeine Z."; RL Acta Crystallogr. B 47:975-986(1991). RN [10] RP X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS). RX MEDLINE=91222450; PubMed=2025410; DOI=10.1107/S0108768190009570; RA Badger J., Harris M.R., Reynolds C.D., Evans A.C., Dodson E.J., RA Dodson G.G., North A.C.T.; RT "Structure of the pig insulin dimer in the cubic crystal."; RL Acta Crystallogr. B 47:127-136(1991). RN [11] RP X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS). RX PubMed=15299880; DOI=10.1107/S0907444997004034; RA Diao J.-S., Wan Z.-L., Chang W.-R., Liang D.-C.; RT "Structure of monomeric porcine DesB1-B2 despentapeptide (B26-B30) RT insulin at 1.65-A resolution."; RL Acta Crystallogr. D 53:507-512(1997). CC -!- FUNCTION: Insulin decreases blood glucose concentration. It CC increases cell permeability to monosaccharides, amino acids and CC fatty acids. It accelerates glycolysis, the pentose phosphate CC cycle, and glycogen synthesis in liver. CC -!- SUBUNIT: Heterodimer of a B chain and an A chain linked by two CC disulfide bonds. CC -!- SUBCELLULAR LOCATION: Secreted. CC -!- SIMILARITY: Belongs to the insulin family. CC -!- WEB RESOURCE: Name=Protein Spotlight; Note=Protein of the 20th CC century - Issue 9 of April 2001; CC URL="http://www.expasy.org/spotlight/back_issues/sptlt009.shtml"; CC ----------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution-NoDerivs License CC ----------------------------------------------------------------------- DR EMBL; AF064555; AAC77920.1; ALT_INIT; mRNA. DR EMBL; AY044828; AAL69550.1; -; Genomic_DNA. DR EMBL; AY242098; AAQ00952.1; -; Genomic_DNA. DR EMBL; AY242099; AAQ00954.1; -; Genomic_DNA. DR EMBL; AY242100; AAQ00957.1; -; Genomic_DNA. DR EMBL; AY242101; AAQ00960.1; -; Genomic_DNA. DR EMBL; AY242102; AAQ00963.1; -; Genomic_DNA. DR EMBL; AY242103; AAQ00966.1; -; Genomic_DNA. DR EMBL; AY242104; AAQ00969.1; -; Genomic_DNA. DR EMBL; AY242105; AAQ00972.1; -; Genomic_DNA. DR EMBL; AY242106; AAQ00975.1; -; Genomic_DNA. DR EMBL; AY242107; AAQ00978.1; -; Genomic_DNA. DR EMBL; AY242108; AAQ00981.1; -; Genomic_DNA. DR EMBL; AY242109; AAQ00983.1; -; Genomic_DNA. DR EMBL; AY242110; AAQ00985.1; -; Genomic_DNA. DR EMBL; AY242111; AAQ00987.1; -; Genomic_DNA. DR EMBL; AY242112; AAQ00990.1; -; Genomic_DNA. DR PIR; A01583; IPPG. DR RefSeq; NP_001103242.1; -. DR UniGene; Ssc.583; -. DR PDB; 1B17; X-ray; 1.70 A; A=88-108, B=25-54. DR PDB; 1B18; X-ray; 1.80 A; A=88-108, B=25-54. DR PDB; 1B19; X-ray; 1.80 A; A=88-108, B=25-54. DR PDB; 1B2A; X-ray; 1.70 A; A=88-108, B=25-54. DR PDB; 1B2B; X-ray; 1.80 A; A=88-108, B=25-54. DR PDB; 1B2C; X-ray; 1.80 A; A=88-108, B=25-54. DR PDB; 1B2D; X-ray; 1.70 A; A=88-108, B=25-54. DR PDB; 1B2E; X-ray; 1.90 A; A=88-108, B=25-54. DR PDB; 1B2F; X-ray; 1.90 A; A=88-108, B=25-54. DR PDB; 1B2G; X-ray; 1.80 A; A=88-108, B=25-54. DR PDB; 1DEI; X-ray; 1.60 A; A/C=88-108, B/D=25-47. DR PDB; 1IZA; X-ray; 2.50 A; A/C=88-108, B/D=25-53. DR PDB; 1IZB; X-ray; 2.00 A; A/C=88-108, B/D=25-53. DR PDB; 1M5A; X-ray; 1.20 A; A/C=79-91, B/D=21-45. DR PDB; 1MPJ; X-ray; 2.30 A; A/C=88-108, B/D=25-54. DR PDB; 1SDB; X-ray; 1.65 A; A=88-108, B=27-49. DR PDB; 1WAV; X-ray; 2.50 A; A/C/E/G/I/K=88-108, B/D/F/H/J/L=25-54. DR PDB; 1ZEI; X-ray; 1.90 A; A/B/C/D/E/F=25-54. DR PDB; 1ZNI; X-ray; 1.50 A; A/C=88-108, B/D=25-54. DR PDB; 2EFA; Neutron; 2.70 A; A=88-108, B=25-54. DR PDB; 2G4M; X-ray; 1.80 A; A=88-108, B=25-54. DR PDB; 2TCI; X-ray; 1.80 A; A/C=88-108, B/D=25-54. DR PDB; 3INS; X-ray; 1.50 A; A/C=88-108, B/D=25-54. DR PDB; 3MTH; X-ray; 1.90 A; A/C=88-108, B/D=25-54. DR PDB; 4INS; X-ray; 1.50 A; A/C=88-108, B/D=25-54. DR PDB; 6INS; X-ray; 2.00 A; E/F=25-53. DR PDB; 7INS; X-ray; 2.00 A; A/C/E=88-108, B/D/F=25-54. DR PDB; 9INS; X-ray; 1.70 A; A=88-108, B=25-54. DR PDBsum; 1B17; -. DR PDBsum; 1B18; -. DR PDBsum; 1B19; -. DR PDBsum; 1B2A; -. DR PDBsum; 1B2B; -. DR PDBsum; 1B2C; -. DR PDBsum; 1B2D; -. DR PDBsum; 1B2E; -. DR PDBsum; 1B2F; -. DR PDBsum; 1B2G; -. DR PDBsum; 1DEI; -. DR PDBsum; 1IZA; -. DR PDBsum; 1IZB; -. DR PDBsum; 1M5A; -. DR PDBsum; 1MPJ; -. DR PDBsum; 1SDB; -. DR PDBsum; 1WAV; -. DR PDBsum; 1ZEI; -. DR PDBsum; 1ZNI; -. DR PDBsum; 2EFA; -. DR PDBsum; 2G4M; -. DR PDBsum; 2TCI; -. DR PDBsum; 3INS; -. DR PDBsum; 3MTH; -. DR PDBsum; 4INS; -. DR PDBsum; 6INS; -. DR PDBsum; 7INS; -. DR PDBsum; 9INS; -. DR SMR; P01315; 25-84. DR GeneID; 397415; -. DR KEGG; ssc:397415; -. DR HOVERGEN; P01315; -. DR LinkHub; P01315; -. DR InterPro; IPR004825; Ins/IGF/relaxin. DR Gene3D; G3DSA:1.10.100.10; Ins/IGF/relaxin; 1. DR Pfam; PF00049; Insulin; 1. DR PRINTS; PR00276; INSULINA. DR PRINTS; PR00277; INSULINB. DR ProDom; PD015667; Mollusc_ins; 1. DR SMART; SM00078; IlGF; 1. DR PROSITE; PS00262; INSULIN; 1. PE 1: Evidence at protein level; KW 3D-structure; Carbohydrate metabolism; KW Cleavage on pair of basic residues; Direct protein sequencing; KW Glucose metabolism; Hormone; Secreted; Signal. FT SIGNAL 1 24 FT PEPTIDE 25 54 Insulin B chain. FT /FTId=PRO_0000015879. FT PROPEP 57 85 C peptide. FT /FTId=PRO_0000015880. FT PEPTIDE 88 108 Insulin A chain. FT /FTId=PRO_0000015881. FT DISULFID 31 94 Interchain (between B and A chains). FT DISULFID 43 107 Interchain (between B and A chains). FT DISULFID 93 98 FT HELIX 28 43 FT HELIX 44 46 FT STRAND 48 50 FT HELIX 89 95 FT HELIX 100 103 FT HELIX 104 106 SQ SEQUENCE 108 AA; 11672 MW; CB4491B429858EBE CRC64; MALWTRLLPL LALLALWAPA PAQAFVNQHL CGSHLVEALY LVCGERGFFY TPKARREAEN PQAGAVELGG GLGGLQALAL EGPPQKRGIV EQCCTSICSL YQLENYCN //