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UniProtKB/Swiss-Prot entry P01190


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name COLI_BOVIN
Primary accession number P01190
Secondary accession numbers Q05B64 Q28166 Q28167 Q28168
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on July 21, 1986 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 74)
Name and origin of the protein
Protein name Corticotropin-lipotropin [Precursor]
Synonyms Pro-opiomelanocortin
POMC
Contains NPP
Melanotropin gamma
     (Gamma-MSH)
Corticotropin
     (Adrenocorticotropic hormone)
     (ACTH)
Melanotropin alpha
     (Alpha-MSH)
Corticotropin-like intermediary peptide
     (CLIP)
Lipotropin beta
     (Beta-LPH)
Lipotropin gamma
     (Gamma-LPH)
Melanotropin beta
     (Beta-MSH)
Beta-endorphin
Met-enkephalin
Gene name
Name: POMC
From
Bos taurus (Bovine) [TaxID: 9913] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1038/278423a0; PubMed=221818 [NCBI, ExPASy, EBI, Israel, Japan]
Nakanishi S., Inoue A., Kita T., Nakamura M., Chang A.C.Y., Cohen S.N., Numa S.;
"Nucleotide sequence of cloned cDNA for bovine corticotropin-beta-lipotropin precursor.";
Nature 278:423-427(1979).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=6249166 [NCBI, ExPASy, EBI, Israel, Japan]
Cohen S.N., Chang A.C.Y., Nakanishi S., Inoue A., Kita T., Nakamura M., Numa S.;
"Studies of cloned DNA encoding the structure for the bovine corticotropin-beta-lipotropin precursor protein.";
Ann. N. Y. Acad. Sci. 343:415-425(1980).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=6263630 [NCBI, ExPASy, EBI, Israel, Japan]
Nakanishi S., Teranishi Y., Watanabe Y., Notake M., Noda M., Kakidani H., Jingami H., Numa S.;
"Isolation and characterization of the bovine corticotropin/beta-lipotropin precursor gene.";
Eur. J. Biochem. 115:429-438(1981).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Hereford;
TISSUE=Hypothalamus;
NIH - Mammalian Gene Collection (MGC) project;
Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [MRNA] OF 75-265.
Rubtsov P.M., Chernov B.K., Gorbulev V.G., Parsadanyan A.S., Sverdlova P.S., Chupeeva V.V., Golova Y.B., Batchikova N.V., Zhvirblis G.S., Skryabin K.G., Baev A.A.;
"Genetic engineering of peptide hormones.";
Mol. Biol. (Mosk.) 19:226-235(1985).
[6]
NUCLEOTIDE SEQUENCE [MRNA] OF 132-200.
PubMed=216007 [NCBI, ExPASy, EBI, Israel, Japan]
Nakanishi G., Inoue A., Kita T., Numa S., Chang A.C.Y., Cohen S.N., Nunberg J., Schimke R.T.;
"Construction of bacterial plasmids that contain the nucleotide sequence for bovine corticotropin-beta-lipotropin precursor.";
Proc. Natl. Acad. Sci. U.S.A. 75:6021-6025(1978).
[7]
PROTEIN SEQUENCE OF 77-87.
DOI=10.1016/0014-5793(81)81081-2; PubMed=7274457 [NCBI, ExPASy, EBI, Israel, Japan]
Boehlen P., Esch F., Shibasaki T., Baird A., Ling N., Guillemin R.;
"Isolation and characterization of a gamma 1-melanotropin-like peptide from bovine neurointermediate pituitary.";
FEBS Lett. 128:67-70(1981).
[8]
PROTEIN SEQUENCE OF 131-144.
PubMed=13642798 [NCBI, ExPASy, EBI, Israel, Japan]
Li C.H.;
"The relation of chemical structure to the biologic activity of pituitary hormones.";
Lab. Invest. 8:574-587(1959).
[9]
PROTEIN SEQUENCE OF 132-170.
Li C.H., Dixon J.S., Chung D.;
"Isolation of melatonin, the pineal gland factor that lightens melanocytes.";
J. Am. Chem. Soc. 80:2587-2588(1958).
[10]
SEQUENCE REVISION (CORTICOTROPIN).
DOI=10.1016/0006-291X(72)90486-X; PubMed=4344689 [NCBI, ExPASy, EBI, Israel, Japan]
Li C.H.;
"Adrenocorticotropin 45. Revised amino acid sequences for sheep and bovine hormones.";
Biochem. Biophys. Res. Commun. 49:835-839(1972).
[11]
PROTEIN SEQUENCE OF 173-265.
Pankov Y.A.;
"Primary structure of the bovine beta-lipotropic hormone.";
Vopr. Med. Khim. 19:330-332(1973).
[12]
PROTEIN SEQUENCE OF 215-232.
Geschwind I.I., Li C.H., Barnafi L.;
"The isolation and structure of a melanocyte-stimulating hormone from bovine pituitary glands.";
J. Am. Chem. Soc. 79:1003-1004(1957).
[13]
PROTEIN SEQUENCE OF 215-232.
PubMed=13348631 [NCBI, ExPASy, EBI, Israel, Japan]
Harris J.I., Roos P.;
"Amino-acid sequence of a melanophore-stimulating peptide.";
Nature 178:90-90(1956).
[14]
PROTEIN SEQUENCE OF 235-239.
PubMed=1065904 [NCBI, ExPASy, EBI, Israel, Japan]
Simantov R., Snyder S.H.;
"Morphine-like peptides in mammalian brain: isolation, structure elucidation, and interactions with the opiate receptor.";
Proc. Natl. Acad. Sci. U.S.A. 73:2515-2519(1976).
[15]
GLYCOSYLATION AT THR-71 AND ASN-91, AND DISULFIDE BONDS.
DOI=10.1016/S0021-9673(01)87458-6; PubMed=4030947 [NCBI, ExPASy, EBI, Israel, Japan]
James S., Bennett H.P.J.;
"Use of reversed-phase and ion-exchange batch extraction in the purification of bovine pituitary peptides.";
J. Chromatogr. A 326:329-338(1985).
[16]
SULFATION AT TYR-200, AND PYROGLUTAMATE FORMATION AT GLU-173.
PubMed=2266117 [NCBI, ExPASy, EBI, Israel, Japan]
Bateman A., Solomon S., Bennett H.P.J.;
"Post-translational modification of bovine pro-opiomelanocortin. Tyrosine sulfation and pyroglutamate formation, a mass spectrometric study.";
J. Biol. Chem. 265:22130-22136(1990).
Comments
  • FUNCTION: ACTH stimulates the adrenal glands to release cortisol.
  • FUNCTION: MSH (melanocyte-stimulating hormone) increases the pigmentation of skin by increasing melanin production in melanocytes.
  • FUNCTION: Beta-endorphin and Met-enkephalin are endogenous opiates.
  • TISSUE SPECIFICITY: ACTH and MSH are produced by the pituitary gland.
  • PTM: Specific enzymatic cleavages at paired basic residues yield the different active peptides.
  • SIMILARITY: Belongs to the POMC family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
V00107; CAA23441.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
V00107; CAA23440.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M25587; AAA30354.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
J00021; AAB59262.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
J00019; AAB59262.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC122728; AAI22729.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M23814; AAA30414.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M10723; AAA30718.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A93206; CTBOP.
RefSeq NP_776576.1; -.
UniGene Bt.8797
3D structure databases
ModBase P01190.
PTM databases
GlycoSuiteDB P01190; -.
Family and domain databases
InterPro IPR001941; Mcortin_ACTH.
IPR013533; Mcortin_ACTH_C.
IPR013531; Mcrtin_ACTH_cent.
IPR013593; Melanocortin_N.
IPR013532; Opioid_neuropept.
Graphical view of domain structure.
PANTHER PTHR11416; Mcortin_ACTH; 1.
Pfam PF00976; ACTH_domain; 1.
PF08384; NPP; 1.
PF08035; Op_neuropeptide; 1.
Pfam graphical view of domain structure.
PRINTS PR00383; MELANOCORTIN.
ProDom PD003250; Mcortin_ACTH; 1.
[Domain structure / List of seq. sharing at least 1 domain]
BLOCKS P01190.
Genome annotation databases
Ensembl ENSBTAG00000007897; Bos taurus. [Contig view]
GeneID 281416; -.
KEGG bta:281416; -.
Phylogenomic databases
HOVERGEN P01190; -.
Other
ProtoNet P01190.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Amidation; Cleavage on pair of basic residues; Direct protein sequencing; Endorphin; Glycoprotein; Hormone; Phosphoprotein; Pyrrolidone carboxylic acid; Signal; Sulfation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    26  26     By similarity. 
PEPTIDE   27   103  77     NPP. PRO_0000024943
PEPTIDE   77    87  11     Melanotropin gamma. PRO_0000024944
PROPEP   106   129  24      PRO_0000024945
PEPTIDE   132   170  39     Corticotropin. PRO_0000024946
PEPTIDE   132   144  13     Melanotropin alpha. PRO_0000024947
PEPTIDE   150   170  21     Corticotropin-like intermediary peptide. PRO_0000024948
PEPTIDE   173   265  93     Lipotropin beta. PRO_0000024949
PEPTIDE   173   232  60     Lipotropin gamma. PRO_0000024950
PEPTIDE   215   232  18     Melanotropin beta. PRO_0000024951
PEPTIDE   235   265  31     Beta-endorphin. PRO_0000024952
PEPTIDE   235   239  5     Met-enkephalin. PRO_0000024953
MOD_RES   87    87        Phenylalanine amide. 
MOD_RES   144   144        Valine amide. 
MOD_RES   162   162        Phosphoserine (By similarity). 
MOD_RES   173   173        Pyrrolidone carboxylic acid (Glu); partial. 
MOD_RES   200   200        Sulfotyrosine. 
CARBOHYD   71    71        O-linked (GalNAc...) [GlycoSuiteDB]. CAR_000202
CARBOHYD   91    91        N-linked (GlcNAc...) [GlycoSuiteDB]. CAR_000034
DISULFID   28    50         
DISULFID   34    46         
CONFLICT   10    10        G -> A (in Ref. 2; AAA30354). 
CONFLICT   139   139        R -> P (in Ref. 2; AAA30354). 
CONFLICT   161   161        Missing (in Ref. 6; AAA30718). 
CONFLICT   188   188        Q -> G (in Ref. 11; may be a typographical error). 
CONFLICT   190   191        ES -> D (in Ref. 6; AAA30718). 
CONFLICT   196   196        A -> P (in Ref. 2; AAA30354). 
Sequence information
Length: 265 AA [This is the length of the unprocessed precursor] Molecular weight: 29260 Da [This is the MW of the unprocessed precursor] CRC64: 303E9A0BCB073B3F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPRLCSSRSG ALLLALLLQA SMEVRGWCLE SSQCQDLTTE SNLLACIRAC KPDLSAETPV 

        70         80         90        100        110        120 
FPGNGDEQPL TENPRKYVMG HFRWDRFGRR NGSSSSGVGG AAQKREEEVA VGEGPGPRGD 

       130        140        150        160        170        180 
DAETGPREDK RSYSMEHFRW GKPVGKKRRP VKVYPNGAED ESAQAFPLEF KRELTGERLE 

       190        200        210        220        230        240 
QARGPEAQAE SAAARAELEY GLVAEAEAEA AEKKDSGPYK MEHFRWGSPP KDKRYGGFMT 

       250        260 
SEKSQTPLVT LFKNAIIKNA HKKGQ 

P01190 in FASTA format

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