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UniProtKB/Swiss-Prot entry P01175


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NEU1_BOVIN
Primary accession number P01175
Secondary accession numbers A5PJ78 P01188
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on January 1, 1988 (Sequence version 1)
Annotations were last modified on    April 29, 2008 (Entry version 73)
Name and origin of the protein
Protein name Oxytocin-neurophysin 1 [Precursor]
Synonym OT-NPI
Contains Oxytocin
     (Ocytocin)
Neurophysin 1
Gene name
Name: OXT
From
Bos taurus (Bovine) [TaxID: 9913] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1038/302342a0; PubMed=6687626 [NCBI, ExPASy, EBI, Israel, Japan]
Land H., Grez M., Ruppert S., Schmale H., Rehbein M., Richter D., Schuetz G.;
"Deduced amino acid sequence from the bovine oxytocin-neurophysin I precursor cDNA.";
Nature 302:342-344(1983).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1038/308554a0; PubMed=6709064 [NCBI, ExPASy, EBI, Israel, Japan]
Ruppert S., Scherer G., Schuetz G.;
"Recent gene conversion involving bovine vasopressin and oxytocin precursor genes suggested by nucleotide sequence.";
Nature 308:554-557(1984).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=3768139 [NCBI, ExPASy, EBI, Israel, Japan]
Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H., Richter D.;
"The neurohypophyseal hormones vasopressin and oxytocin. Precursor structure, synthesis and regulation.";
Biol. Chem. Hoppe-Seyler 367:695-704(1986).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=Hereford;
TISSUE=Hypothalamus;
NIH - Mammalian Gene Collection (MGC) project;
Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [MRNA] OF 15-125.
PubMed=6209133 [NCBI, ExPASy, EBI, Israel, Japan]
Ivell R., Richter D.;
"The gene for the hypothalamic peptide hormone oxytocin is highly expressed in the bovine corpus luteum: biosynthesis, structure and sequence analysis.";
EMBO J. 3:2351-2354(1984).
[6]
PROTEIN SEQUENCE OF 20-28, DISULFIDE BONDS, AND AMIDATION AT GLY-28.
PubMed=13129273 [NCBI, ExPASy, EBI, Israel, Japan]
du Vigneaud V., Ressler C., Trippett S.;
"The sequence of amino acids in oxytocin, with a proposal for the structure of oxytocin.";
J. Biol. Chem. 205:949-957(1953).
[7]
PROTEIN SEQUENCE OF 20-28.
Tuppy H., Michl H.;
"About the chemical structure of oxytocin.";
Monatsh. Chem. 84:1011-1020(1953).
[8]
PROTEIN SEQUENCE OF 32-124.
PubMed=670174 [NCBI, ExPASy, EBI, Israel, Japan]
Schlesinger D.H., Audhya T.K., Walter R.;
"Complete amino acid sequence of bovine neurophysin-I. A major secretory product of the posterior pituitary.";
J. Biol. Chem. 253:5019-5024(1978).
[9]
PROTEIN SEQUENCE OF 32-124.
DOI=10.1016/0014-5793(79)80146-5; PubMed=428540 [NCBI, ExPASy, EBI, Israel, Japan]
Chauvet M.-T., Codogno P., Chauvet J., Acher R.;
"Comparison between MSEL- and VLDV-neurophysins. Complete amino acid sequences of porcine and bovine VLDV-neurophysins.";
FEBS Lett. 98:37-40(1979).
[10]
DISULFIDE BONDS.
PubMed=2911588 [NCBI, ExPASy, EBI, Israel, Japan]
Burman S., Wellner D., Chait B., Chaudhary T., Breslow E.;
"Complete assignment of neurophysin disulfides indicates pairing in two separate domains.";
Proc. Natl. Acad. Sci. U.S.A. 86:429-433(1989).
[11]
X-RAY CRYSTALLOGRAPHY (1.04 ANGSTROMS) OF OXYTOCIN, DISULFIDE BOND, AND AMIDATION AT GLY-28.
PubMed=3008332 [NCBI, ExPASy, EBI, Israel, Japan]
Wood S.P., Tickle I.J., Treharne A.M., Pitts J.E., Mascarenhas Y., Li J.Y., Husain J., Cooper S., Blundell T.L., Hruby V.J.;
"Crystal structure analysis of deamino-oxytocin: conformational flexibility and receptor binding.";
Science 232:633-636(1986).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X00502; CAA25194.1; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M25648; AAA30680.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC141997; AAI41998.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
V00114; CAA23448.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
V00114; CAA23450.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
V00114; CAA23449.1; ALT_SEQ; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X00950; CAA25462.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S07332; NFBO1.
RefSeq NP_789825.1; -.
UniGene Bt.183
3D structure databases
PDB
1L5C; NMR; -; A=32-123.[ExPASy / RCSB / EBI]
1L5D; NMR; -; A=32-123.[ExPASy / RCSB / EBI]
1XY1; X-ray; 1.04 A; A/B=21-28.[ExPASy / RCSB / EBI]
1XY2; X-ray; 1.20 A; A=21-28.[ExPASy / RCSB / EBI]
2HNU; X-ray; 2.00 A; A/B/C/D/E=38-118.[ExPASy / RCSB / EBI]
2HNV; X-ray; 2.50 A; A/B/C/D/E=38-118.[ExPASy / RCSB / EBI]
2HNW; X-ray; 2.90 A; A/B/C/D/E=38-117.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1L5C; -.
1L5D; -.
1XY1; -.
1XY2; -.
2HNU; -.
2HNV; -.
2HNW; -.
ModBase P01175.
Family and domain databases
InterPro IPR000981; Neurhyp_horm.
IPR016321; Nonapeptide_hormone_prcur.
Graphical view of domain structure.
Gene3D G3DSA:2.60.9.10; Neurhyp_horm; 1.
Pfam PF00220; Hormone_4; 1.
PF00184; Hormone_5; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF001815; Nonapeptide_hormone_precursor; 1.
PRINTS PR00831; NEUROPHYSIN.
ProDom PD001676; Neurhyp_horm; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00003; NH; 1.
SMART graphical view of domain structure.
PROSITE PS00264; NEUROHYPOPHYS_HORM; 1.
BLOCKS P01175.
Genome annotation databases
Ensembl ENSBTAG00000008026; Bos taurus. [Contig view]
GeneID 280888; -.
KEGG bta:280888; -.
Other
LinkHub P01175; -.
ProtoNet P01175.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Amidation; Cleavage on pair of basic residues; Direct protein sequencing; Hormone; Secreted; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    19  19      
PEPTIDE   20    28  9     Oxytocin. PRO_0000020491
CHAIN   32   125  94     Neurophysin 1. PRO_0000020492
MOD_RES   28    28        Glycine amide. 
DISULFID   20    25         
DISULFID   41    85         
DISULFID   44    58         
DISULFID   52    75         
DISULFID   59    65         
DISULFID   92   104         
DISULFID   98   116         
DISULFID   105   110         
CONFLICT   124   124        Q -> L (in Ref. 9; AA sequence). 
STRAND   36    38  3      
STRAND   43    45  3      
HELIX   46    48  3      
STRAND   50    54  5      
STRAND   57    60  4      
TURN   61    63  3      
STRAND   64    69  6      
HELIX   70    80  11      
STRAND   89    92  4      
TURN   93    95  3      
STRAND   96   100  5      
STRAND   103   106  4      
STRAND   109   112  4      
HELIX   114   116  3      
Sequence information
Length: 125 AA [This is the length of the unprocessed precursor] Molecular weight: 12665 Da [This is the MW of the unprocessed precursor] CRC64: 3B4E4E79A0B7F032 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAGSSLACCL LGLLALTSAC YIQNCPLGGK RAVLDLDVRT CLPCGPGGKG RCFGPSICCG 

        70         80         90        100        110        120 
DELGCFVGTA EALRCQEENY LPSPCQSGQK PCGSGGRCAA AGICCSPDGC HEDPACDPEA 


AFSQH 

P01175 in FASTA format

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