[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1093/nar/11.20.7145; PubMed=6314278 [NCBI, ExPASy, EBI, Israel, Japan] Armstrong J., Brown R.S., Tsugita A.; "Primary structure and genetic organization of phage T4 DNA ligase."; Nucleic Acids Res. 11:7145-7156(1983).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1128/MMBR.67.1.86-156.2003; PubMed=12626685 [NCBI, ExPASy, EBI, Israel, Japan] Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.; "Bacteriophage T4 genome."; Microbiol. Mol. Biol. Rev. 67:86-156(2003).

Comments

FUNCTION: DNA ligase, which is expressed in the early stage of lytic development, has been implicated in T4 DNA synthesis and genetic recombination. It may also play a role in T4 DNA repair.
CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
WEB RESOURCE: Name=Worthington enzyme manual; URL="http://www.worthington-biochem.com/DNAT4L/";.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X00039; CAA24921.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF158101; AAD42440.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A01201; LQBP34.

RefSeq

NP_049813.1; -.

3D structure databases

ModBase

P00970.

Family and domain databases

InterPro

IPR012310; DNA_ligase_A_M.
IPR016059; DNA_ligase_CS.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.50.140; OB_NA_bd_sub; 1.

Pfam

PF01068; DNA_ligase_A_M; 1.
Pfam graphical view of domain structure.

PROSITE

PS00697; DNA_LIGASE_A1; 1.
PS00333; DNA_LIGASE_A2; 1.

Genome annotation databases

GeneID

1258680; -.

GenomeReviews

AF158101_GR; T4p203.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 487`	`487`	`DNA ligase.`	`PRO_0000059595`
`ACT_SITE`	`159 159`		`N6-AMP-lysine intermediate (By similarity).`

Sequence information

Length: 487 AA [This is the length of the unprocessed precursor]

Molecular weight: 55293 Da [This is the MW of the unprocessed precursor]

CRC64: 39F8718DD067F8AA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MILKILNEIA SIGSTKQKQA ILEKNKDNEL LKRVYRLTYS RGLQYYIKKW PKPGIATQSF 

        70         80         90        100        110        120 
GMLTLTDMLD FIEFTLATRK LTGNAAIEEL TGYITDGKKD DVEVLRRVMM RDLECGASVS 

       130        140        150        160        170        180 
IANKVWPGLI PEQPQMLASS YDEKGINKNI KFPAFAQLKA DGARCFAEVR GDELDDVRLL 

       190        200        210        220        230        240 
SRAGNEYLGL DLLKEELIKM TAEARQIHPE GVLIDGELVY HEQVKKEPEG LDFLFDAYPE 

       250        260        270        280        290        300 
NSKAKEFAEV AESRTASNGI ANKSLKGTIS EKEAQCMKFQ VWDYVPLVEI YSLPAFRLKY 

       310        320        330        340        350        360 
DVRFSKLEQM TSGYDKVILI ENQVVNNLDE AKVIYKKYID QGLEGIILKN IDGLWENARS 

       370        380        390        400        410        420 
KNLYKFKEVI DVDLKIVGIY PHRKDPTKAG GFILESECGK IKVNAGSGLK DKAGVKSHEL 

       430        440        450        460        470        480 
DRTRIMENQN YYIGKILECE CNGWLKSDGR TDYVKLFLPI AIRLREDKTK ANTFEDVFGD 


FHEVTGL

P00970 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools