ID   DNLI_BPT7               Reviewed;         359 AA.
AC   P00969;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   02-SEP-2008, entry version 70.
DE   RecName: Full=DNA ligase;
DE            EC=6.5.1.1;
DE   AltName: Full=Polydeoxyribonucleotide synthase [ATP];
GN   Name=1.3;
OS   Enterobacteria phage T7 (Bacteriophage T7).
OC   Viruses; dsDNA viruses, no RNA stage; Caudovirales; Podoviridae;
OC   Autographivirinae; T7-like viruses.
OX   NCBI_TaxID=10760;
OH   NCBI_TaxID=562; Escherichia coli.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=83241725; PubMed=6864790; DOI=10.1016/S0022-2836(83)80282-4;
RA   Dunn J.J., Studier F.W.;
RT   "Complete nucleotide sequence of bacteriophage T7 DNA and the
RT   locations of T7 genetic elements.";
RL   J. Mol. Biol. 166:477-535(1983).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=82078034; PubMed=7310871; DOI=10.1016/0022-2836(81)90178-9;
RA   Dunn J.J., Studier F.W.;
RT   "Nucleotide sequence from the genetic left end of bacteriophage T7 DNA
RT   to the beginning of gene 4.";
RL   J. Mol. Biol. 148:303-330(1981).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 278-359.
RX   MEDLINE=81053683; PubMed=7001354; DOI=10.1093/nar/8.10.2119;
RA   Dunn J.J., Studier F.W.;
RT   "The transcription termination site at the end of the early region of
RT   bacteriophage T7 DNA.";
RL   Nucleic Acids Res. 8:2119-2132(1980).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-5.
RX   MEDLINE=81054683; PubMed=6254001;
RA   Saito H., Tabor S., Tamanoi F., Richardson C.C.;
RT   "Nucleotide sequence of the primary origin of bacteriophage T7 DNA
RT   replication: relationship to adjacent genes and regulatory elements.";
RL   Proc. Natl. Acad. Sci. U.S.A. 77:3917-3921(1980).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS).
RX   MEDLINE=96222302; PubMed=8653795; DOI=10.1016/S0092-8674(00)81260-X;
RA   Subramanya H.S., Doherty A.J., Ashford S.R., Wigley D.B.;
RT   "Crystal structure of an ATP-dependent DNA ligase from bacteriophage
RT   T7.";
RL   Cell 85:607-615(1996).
CC   -!- FUNCTION: DNA ligase, which is expressed in the early stage of
CC       lytic development, has been implicated in T7 DNA synthesis and
CC       genetic recombination. It may also play a role in T7 DNA repair.
CC   -!- CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) +
CC       (deoxyribonucleotide)(m) = AMP + diphosphate +
CC       (deoxyribonucleotide)(n+m).
CC   -!- SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
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DR   EMBL; V01124; CAA24322.1; -; Genomic_DNA.
DR   EMBL; V01126; CAA24326.1; -; Genomic_DNA.
DR   EMBL; V01127; CAA24336.1; -; Genomic_DNA.
DR   EMBL; V01146; CAA24393.1; -; Genomic_DNA.
DR   PIR; E94615; LQBP37.
DR   RefSeq; NP_041963.1; -.
DR   PDB; 1A0I; X-ray; 2.60 A; A=3-349.
DR   PDBsum; 1A0I; -.
DR   GeneID; 1261055; -.
DR   GenomeReviews; V01146_GR; 1.3.
DR   LinkHub; P00969; -.
DR   InterPro; IPR012310; DNA_ligase_A_M.
DR   InterPro; IPR016059; DNA_ligase_CS.
DR   InterPro; IPR016306; DNA_ligase_phage.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Gene3D; G3DSA:2.40.50.140; OB_NA_bd_sub; 1.
DR   Pfam; PF01068; DNA_ligase_A_M; 1.
DR   PIRSF; PIRSF001600; DNA_ligase_phage_T3; 1.
DR   PROSITE; PS00697; DNA_LIGASE_A1; 1.
DR   PROSITE; PS00333; DNA_LIGASE_A2; 1.
DR   PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; ATP-binding; DNA damage; DNA recombination; DNA repair;
KW   DNA replication; Ligase; Nucleotide-binding.
FT   CHAIN         1    359       DNA ligase.
FT                                /FTId=PRO_0000059598.
FT   ACT_SITE     34     34       N6-AMP-lysine intermediate (By
FT                                similarity).
FT   STRAND        9     13
FT   HELIX        16     26
FT   STRAND       27     33
FT   STRAND       37     45
FT   STRAND       48     53
FT   HELIX        61     66
FT   HELIX        71     78
FT   STRAND       87     99
FT   TURN        101    103
FT   HELIX       104    109
FT   STRAND      115    119
FT   TURN        141    143
FT   STRAND      144    153
FT   HELIX       154    159
FT   STRAND      163    166
FT   HELIX       167    184
FT   STRAND      188    192
FT   STRAND      195    200
FT   HELIX       201    212
FT   TURN        213    215
FT   STRAND      219    222
FT   STRAND      230    240
FT   STRAND      243    253
FT   TURN        257    259
FT   STRAND      267    271
FT   STRAND      277    281
FT   HELIX       286    299
FT   TURN        300    302
FT   TURN        321    324
FT   STRAND      326    334
FT   STRAND      340    342
FT   STRAND      344    348
SQ   SEQUENCE   359 AA;  41133 MW;  43DA453B71BDF8DC CRC64;
     MMNIKTNPFK AVSFVESAIK KALDNAGYLI AEIKYDGVRG NICVDNTANS YWLSRVSKTI
     PALEHLNGFD VRWKRLLNDD RCFYKDGFML DGELMVKGVD FNTGSGLLRT KWTDTKNQEF
     HEELFVEPIR KKDKVPFKLH TGHLHIKLYA ILPLHIVESG EDCDVMTLLM QEHVKNMLPL
     LQEYFPEIEW QAAESYEVYD MVELQQLYEQ KRAEGHEGLI VKDPMCIYKR GKKSGWWKMK
     PENEADGIIQ GLVWGTKGLA NEGKVIGFEV LLESGRLVNA TNISRALMDE FTETVKEATL
     SQWGFFSPYG IGDNDACTIN PYDGWACQIS YMEETPDGSL RHPSFVMFRG TEDNPQEKM
//