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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=NCIMB 6816 / CCM 2182 / NCDO 727;
DOI=10.1093/nar/13.24.8913; PubMed=3001653 [NCBI, ExPASy, EBI, Israel, Japan]
Jacobs M.,
Eliasson M.,
Uhlen M.,
Flock J.-I.;
"Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis.";
Nucleic Acids Res. 13:8913-8926(1985).
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[2]
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PROTEIN SEQUENCE OF 106-379.
PubMed=4967581 [NCBI, ExPASy, EBI, Israel, Japan]
Smith E.L.,
Delange R.J.,
Evans W.H.,
Landon M.,
Markland F.S.;
"Subtilisin Carlsberg. V. The complete sequence; comparison with subtilisin BPN'; evolutionary relationships.";
J. Biol. Chem. 243:2184-2191(1968).
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[3]
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X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF MUTANT WITH SELENOCYSTEINE-325.
DOI=10.1021/bi00075a007; PubMed=8512925 [NCBI, ExPASy, EBI, Israel, Japan]
Syed R.,
Wu Z.P.,
Hogle J.M.,
Hilvert D.;
"Crystal structure of selenosubtilisin at 2.0-A resolution.";
Biochemistry 32:6157-6164(1993).
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[4]
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X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 106-379.
DOI=10.1021/bi971166o; PubMed=9425066 [NCBI, ExPASy, EBI, Israel, Japan]
Stoll V.S.,
Eger B.T.,
Hynes R.C.,
Martichonok V.,
Jones J.B.,
Pai E.F.;
"Differences in binding modes of enantiomers of 1-acetamido boronic acid based protease inhibitors: crystal structures of gamma-chymotrypsin and subtilisin Carlsberg complexes.";
Biochemistry 37:451-462(1998).
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- FUNCTION: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides.
- CATALYTIC ACTIVITY: Hydrolysis of proteins with broad specificity for peptide bonds, and a preference for a large uncharged residue in P1. Hydrolyzes peptide amides.
- COFACTOR: Binds 2 calcium ions per subunit.
- SUBCELLULAR LOCATION: Secreted.
- BIOTECHNOLOGY: Used as a detergent protease. Sold under the name Alcalase by Novozymes.
- MISCELLANEOUS: Secretion of subtilisin is associated with onset of sporulation, and many mutations which block sporulation at early stages affect expression levels of subtilisin. However, subtilisin is not necessary for normal sporulation.
- SIMILARITY: Belongs to the peptidase S8 family [view classification].
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 379 AA [This is the length of the unprocessed precursor] |
Molecular weight: 38908 Da [This is the MW of the unprocessed precursor] |
CRC64: F19A6DC5761FB504 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MMRKKSFWLG MLTAFMLVFT MAFSDSASAA QPAKNVEKDY IVGFKSGVKT ASVKKDIIKE
70 80 90 100 110 120
SGGKVDKQFR IINAAKAKLD KEALKEVKND PDVAYVEEDH VAHALAQTVP YGIPLIKADK
130 140 150 160 170 180
VQAQGFKGAN VKVAVLDTGI QASHPDLNVV GGASFVAGEA YNTDGNGHGT HVAGTVAALD
190 200 210 220 230 240
NTTGVLGVAP SVSLYAVKVL NSSGSGTYSG IVSGIEWATT NGMDVINMSL GGPSGSTAMK
250 260 270 280 290 300
QAVDNAYARG VVVVAAAGNS GSSGNTNTIG YPAKYDSVIA VGAVDSNSNR ASFSSVGAEL
310 320 330 340 350 360
EVMAPGAGVY STYPTSTYAT LNGTSMASPH VAGAAALILS KHPNLSASQV RNRLSSTATY
370
LGSSFYYGKG LINVEAAAQ
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P00780 in FASTA format |
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