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UniProtKB/Swiss-Prot entry P00772

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ELA1_PIG
Primary accession number P00772
Secondary accession number Q29625
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on April 1, 1988 (Sequence version 1)
Annotations were last modified on    September 23, 2008 (Entry version 99)
Name and origin of the protein
Protein name Elastase-1 [Precursor]
Synonym EC 3.4.21.36
Gene name
Name: ELA1
From
Sus scrofa (Pig) [TaxID: 9823] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae; Sus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE.
PubMed=3528137 [NCBI, ExPASy, EBI, Israel, Japan]
Shirasu Y., Yoshida H., Mikayama T., Matsuki S., Tanaka J., Ikenaga H.;
"Isolation and expression in Escherichia coli of a cDNA clone encoding porcine pancreatic elastase.";
J. Biochem. 99:1707-1712(1986).
[2]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=3648024 [NCBI, ExPASy, EBI, Israel, Japan]
Tani T., Kawashima I., Furukawa H., Ohmine T., Takiguchi Y.;
"Characterization of a silent gene for human pancreatic elastase I: structure of the 5'-flanking region.";
J. Biochem. 101:591-599(1987).
[3]
 PROTEIN SEQUENCE OF 27-266.
PubMed=4578945 [NCBI, ExPASy, EBI, Israel, Japan]
Shotton D.M., Hartley B.S.;
"Evidence for the amino acid sequence of porcine pancreatic elastase.";
Biochem. J. 131:643-675(1973).
[4]
 PROTEIN SEQUENCE OF 27-266.
DOI=10.1038/225802a0; PubMed=5415108 [NCBI, ExPASy, EBI, Israel, Japan]
Shotton D.M., Hartley B.S.;
"Amino-acid sequence of porcine pancreatic elastase and its homologies with other serine proteinases.";
Nature 225:802-806(1970).
[5]
 X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
DOI=10.1016/0022-2836(78)90412-6; PubMed=628010 [NCBI, ExPASy, EBI, Israel, Japan]
Sawyer L., Shotton D.M., Campbell J.W., Wendell P.L., Muirhead H., Watson H.C., Diamond R., Ladner R.C.;
"The atomic structure of crystalline porcine pancreatic elastase at 2.5-A resolution: comparisons with the structure of alpha-chymotrypsin.";
J. Mol. Biol. 118:137-208(1978).
[6]
 X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS), AND ACTIVE SITE.
DOI=10.1038/225811a0; PubMed=5415110 [NCBI, ExPASy, EBI, Israel, Japan]
Shotton D.M., Watson H.C.;
"Three-dimensional structure of tosyl-elastase.";
Nature 225:811-816(1970).
[7]
 X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF COMPLEX WITH INHIBITOR.
DOI=10.1016/S0969-2126(00)00068-X; PubMed=7922044 [NCBI, ExPASy, EBI, Israel, Japan]
Huang K., Strynadka N.C., Bernard V.D., Peanasky R.J., James M.N.;
"The molecular structure of the complex of Ascaris chymotrypsin/elastase inhibitor with porcine elastase.";
Structure 2:679-689(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X04036; CAA27670.1; -; Other_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D00160; BAA00118.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR JS0013; ELPG.
RefSeq NP_998988.1; -.
UniGene Ssc.5575
3D structure databases
PDB
1B0E; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
1BMA; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
1BTU; X-ray; 1.60 A; A=27-266.[ExPASy / RCSB / EBI]
1C1M; X-ray; 2.20 A; A=27-266.[ExPASy / RCSB / EBI]
1E34; X-ray; 1.80 A; B=27-266.[ExPASy / RCSB / EBI]
1E35; X-ray; 1.90 A; B=27-266.[ExPASy / RCSB / EBI]
1E36; X-ray; 1.70 A; B=27-266.[ExPASy / RCSB / EBI]
1E37; X-ray; 1.75 A; B=27-266.[ExPASy / RCSB / EBI]
1E38; X-ray; 1.70 A; B=27-266.[ExPASy / RCSB / EBI]
1EAI; X-ray; 2.40 A; A/B=27-266.[ExPASy / RCSB / EBI]
1EAS; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
1EAT; X-ray; 2.00 A; A=27-266.[ExPASy / RCSB / EBI]
1EAU; X-ray; 2.00 A; A=27-266.[ExPASy / RCSB / EBI]
1ELA; X-ray; 2.00 A; A=27-266.[ExPASy / RCSB / EBI]
1ELB; X-ray; 2.10 A; A=27-266.[ExPASy / RCSB / EBI]
1ELC; X-ray; 1.75 A; A=27-266.[ExPASy / RCSB / EBI]
1ELD; X-ray; 2.00 A; E=27-266.[ExPASy / RCSB / EBI]
1ELE; X-ray; 2.00 A; E=27-266.[ExPASy / RCSB / EBI]
1ELF; X-ray; 1.70 A; A=27-266.[ExPASy / RCSB / EBI]
1ELG; X-ray; 1.65 A; A=27-266.[ExPASy / RCSB / EBI]
1ESA; X-ray; 1.65 A; A=27-266.[ExPASy / RCSB / EBI]
1ESB; X-ray; 2.30 A; A=27-266.[ExPASy / RCSB / EBI]
1EST; X-ray; 2.50 A; A=27-266.[ExPASy / RCSB / EBI]
1FLE; X-ray; 1.90 A; E=27-266.[ExPASy / RCSB / EBI]
1FZZ; X-ray; 1.86 A; A=27-266.[ExPASy / RCSB / EBI]
1GVK; X-ray; 0.94 A; B=27-266.[ExPASy / RCSB / EBI]
1GWA; X-ray; 1.85 A; A=27-266.[ExPASy / RCSB / EBI]
1H9L; X-ray; 1.67 A; B=27-266.[ExPASy / RCSB / EBI]
1HAX; X-ray; 1.60 A; B=27-266.[ExPASy / RCSB / EBI]
1HAY; X-ray; 1.70 A; B=27-266.[ExPASy / RCSB / EBI]
1HAZ; X-ray; 1.40 A; B=27-266.[ExPASy / RCSB / EBI]
1HB0; X-ray; 2.05 A; B=27-266.[ExPASy / RCSB / EBI]
1HV7; X-ray; 1.70 A; A=27-266.[ExPASy / RCSB / EBI]
1INC; X-ray; 1.94 A; A=27-266.[ExPASy / RCSB / EBI]
1JIM; X-ray; 2.31 A; A=27-266.[ExPASy / RCSB / EBI]
1L0Z; X-ray; 1.50 A; A=27-266.[ExPASy / RCSB / EBI]
1L1G; X-ray; 1.50 A; A=27-266.[ExPASy / RCSB / EBI]
1LKA; X-ray; 1.70 A; A=27-266.[ExPASy / RCSB / EBI]
1LKB; X-ray; 1.70 A; A=27-266.[ExPASy / RCSB / EBI]
1LVY; X-ray; 1.87 A; A=27-266.[ExPASy / RCSB / EBI]
1MCV; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
1MMJ; X-ray; 2.20 A; N=27-266.[ExPASy / RCSB / EBI]
1NES; X-ray; 1.65 A; E=27-266.[ExPASy / RCSB / EBI]
1OKX; X-ray; 2.80 A; A/B=27-266.[ExPASy / RCSB / EBI]
1QGF; X-ray; 1.70 A; A=27-266.[ExPASy / RCSB / EBI]
1QIX; X-ray; 1.90 A; B=27-266.[ExPASy / RCSB / EBI]
1QNJ; X-ray; 1.10 A; A=27-266.[ExPASy / RCSB / EBI]
1QR3; X-ray; 1.60 A; E=27-266.[ExPASy / RCSB / EBI]
1UO6; X-ray; 1.65 A; A=27-266.[ExPASy / RCSB / EBI]
1UVO; X-ray; 1.85 A; A=27-266.[ExPASy / RCSB / EBI]
1UVP; X-ray; 1.85 A; A=27-266.[ExPASy / RCSB / EBI]
2A7C; X-ray; 1.65 A; A=27-266.[ExPASy / RCSB / EBI]
2A7J; X-ray; 1.65 A; A=27-266.[ExPASy / RCSB / EBI]
2BB4; X-ray; 1.60 A; A=27-266.[ExPASy / RCSB / EBI]
2BD2; X-ray; 1.70 A; A=27-266.[ExPASy / RCSB / EBI]
2BD3; X-ray; 1.60 A; A=27-266.[ExPASy / RCSB / EBI]
2BD4; X-ray; 1.70 A; A=27-266.[ExPASy / RCSB / EBI]
2BD5; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
2BD7; X-ray; 1.60 A; A=27-266.[ExPASy / RCSB / EBI]
2BD8; X-ray; 1.70 A; A=27-266.[ExPASy / RCSB / EBI]
2BD9; X-ray; 1.90 A; A=27-266.[ExPASy / RCSB / EBI]
2BDA; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
2BDB; X-ray; 1.70 A; A=27-266.[ExPASy / RCSB / EBI]
2BDC; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
2BLO; X-ray; 1.33 A; A=27-266.[ExPASy / RCSB / EBI]
2BLQ; X-ray; 1.33 A; A=27-266.[ExPASy / RCSB / EBI]
2CV3; X-ray; 1.90 A; A=27-266.[ExPASy / RCSB / EBI]
2D26; X-ray; 3.30 A; C=27-266.[ExPASy / RCSB / EBI]
2DE8; X-ray; 1.50 A; A=27-266.[ExPASy / RCSB / EBI]
2DE9; X-ray; 1.30 A; A=27-266.[ExPASy / RCSB / EBI]
2EST; X-ray; 2.50 A; E=27-266.[ExPASy / RCSB / EBI]
2FO9; X-ray; 2.00 A; A=27-266.[ExPASy / RCSB / EBI]
2FOA; X-ray; 1.90 A; A=27-266.[ExPASy / RCSB / EBI]
2FOB; X-ray; 1.90 A; A=27-266.[ExPASy / RCSB / EBI]
2FOC; X-ray; 2.00 A; A=27-266.[ExPASy / RCSB / EBI]
2FOD; X-ray; 2.00 A; A=27-266.[ExPASy / RCSB / EBI]
2FOE; X-ray; 2.20 A; A=27-266.[ExPASy / RCSB / EBI]
2FOF; X-ray; 2.20 A; A=27-266.[ExPASy / RCSB / EBI]
2FOG; X-ray; 1.90 A; A=27-266.[ExPASy / RCSB / EBI]
2FOH; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
2G4T; X-ray; 2.15 A; A=27-266.[ExPASy / RCSB / EBI]
2G4U; X-ray; 1.84 A; A=27-266.[ExPASy / RCSB / EBI]
2H1U; X-ray; 1.60 A; A=27-266.[ExPASy / RCSB / EBI]
2IOT; X-ray; 1.60 A; A=27-266.[ExPASy / RCSB / EBI]
2OQU; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
2V0B; X-ray; 1.65 A; A=27-266.[ExPASy / RCSB / EBI]
2V35; X-ray; 1.67 A; A=27-266.[ExPASy / RCSB / EBI]
3EST; X-ray; 1.65 A; A=27-266.[ExPASy / RCSB / EBI]
4EST; X-ray; 1.78 A; E=27-266.[ExPASy / RCSB / EBI]
5EST; X-ray; 2.09 A; E=27-266.[ExPASy / RCSB / EBI]
6EST; X-ray; 1.80 A; A=27-266.[ExPASy / RCSB / EBI]
7EST; X-ray; 1.80 A; E=27-266.[ExPASy / RCSB / EBI]
8EST; X-ray; 1.78 A; E=27-266.[ExPASy / RCSB / EBI]
9EST; X-ray; 1.90 A; A=27-266.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1B0E; -.
1BMA; -.
1BTU; -.
1C1M; -.
1E34; -.
1E35; -.
1E36; -.
1E37; -.
1E38; -.
1EAI; -.
1EAS; -.
1EAT; -.
1EAU; -.
1ELA; -.
1ELB; -.
1ELC; -.
1ELD; -.
1ELE; -.
1ELF; -.
1ELG; -.
1ESA; -.
1ESB; -.
1EST; -.
1FLE; -.
1FZZ; -.
1GVK; -.
1GWA; -.
1H9L; -.
1HAX; -.
1HAY; -.
1HAZ; -.
1HB0; -.
1HV7; -.
1INC; -.
1JIM; -.
1L0Z; -.
1L1G; -.
1LKA; -.
1LKB; -.
1LVY; -.
1MCV; -.
1MMJ; -.
1NES; -.
1OKX; -.
1QGF; -.
1QIX; -.
1QNJ; -.
1QR3; -.
1UO6; -.
1UVO; -.
1UVP; -.
2A7C; -.
2A7J; -.
2BB4; -.
2BD2; -.
2BD3; -.
2BD4; -.
2BD5; -.
2BD7; -.
2BD8; -.
2BD9; -.
2BDA; -.
2BDB; -.
2BDC; -.
2BLO; -.
2BLQ; -.
2CV3; -.
2D26; -.
2DE8; -.
2DE9; -.
2EST; -.
2FO9; -.
2FOA; -.
2FOB; -.
2FOC; -.
2FOD; -.
2FOE; -.
2FOF; -.
2FOG; -.
2FOH; -.
2G4T; -.
2G4U; -.
2H1U; -.
2IOT; -.
2OQU; -.
2V0B; -.
2V35; -.
3EST; -.
4EST; -.
5EST; -.
6EST; -.
7EST; -.
8EST; -.
9EST; -.
ModBase P00772.
Protein-protein interaction databases
DIP DIP:378N; -.
IntAct P00772; -.
Protein family/group databases
MEROPS S01.153; -.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR001254; Peptidase_S1_S6.
IPR001314; Peptidase_S1A.
Graphical view of domain structure.
Pfam PF00089; Trypsin; 1.
Pfam graphical view of domain structure.
PRINTS PR00722; CHYMOTRYPSIN.
SMART SM00020; Tryp_SPc; 1.
SMART graphical view of domain structure.
PROSITE PS50240; TRYPSIN_DOM; 1.
PS00134; TRYPSIN_HIS; 1.
PS00135; TRYPSIN_SER; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P00772.
ProtoNet P00772.
Other
SWISS-3DIMAGE P00772.
Genome annotation databases
GeneID 396766; -.
KEGG ssc:396766; -.
Phylogenomic databases
HOVERGEN P00772; -.
Other
LinkHub P00772; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Calcium; Direct protein sequencing; Hydrolase; Metal-binding; Protease; Secreted; Serine protease; Signal; Zymogen.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    16  16      
PROPEP   17    26  10     Activation peptide. PRO_0000027681
CHAIN   27   266  240     Elastase-1. PRO_0000027682
DOMAIN   27   264  238     Peptidase S1. 
ACT_SITE   71    71        Charge relay system. 
ACT_SITE   119   119        Charge relay system. 
ACT_SITE   214   214        Charge relay system. 
METAL   85    85        Calcium. 
METAL   90    90        Calcium; via carbonyl oxygen. 
METAL   95    95        Calcium. 
DISULFID   56    72         
DISULFID   153   220         
DISULFID   184   200         
DISULFID   210   240         
CONFLICT   92    92        D -> N (in Ref. 3; AA sequence). 
CONFLICT   126   126        A -> G (in Ref. 2; BAA00118). 
CONFLICT   184   184        C -> L (in Ref. 2; BAA00118). 
CONFLICT   204   204        D -> N (in Ref. 3; AA sequence). 
STRAND   41    48  8      
STRAND   51    62  12      
STRAND   65    68  4      
HELIX   70    73  4      
STRAND   79    84  6      
STRAND   96   105  10      
HELIX   114   116  3      
STRAND   121   127  7      
STRAND   153   158  6      
STRAND   160   162  3      
STRAND   172   175  4      
HELIX   181   184  4      
TURN   187   190  4      
HELIX   191   193  3      
STRAND   198   201  4      
STRAND   204   206  3      
STRAND   217   222  6      
STRAND   225   234  10      
STRAND   239   241  3      
STRAND   247   251  5      
HELIX   252   254  3      
HELIX   256   265  10      
Sequence information
Length: 266 AA [This is the length of the unprocessed precursor] Molecular weight: 28821 Da [This is the MW of the unprocessed precursor] CRC64: BF07D6855BB50FE2 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MLRLLVVASL VLYGHSTQDF PETNARVVGG TEAQRNSWPS QISLQYRSGS SWAHTCGGTL 

        70         80         90        100        110        120 
IRQNWVMTAA HCVDRELTFR VVVGEHNLNQ NDGTEQYVGV QKIVVHPYWN TDDVAAGYDI 

       130        140        150        160        170        180 
ALLRLAQSVT LNSYVQLGVL PRAGTILANN SPCYITGWGL TRTNGQLAQT LQQAYLPTVD 

       190        200        210        220        230        240 
YAICSSSSYW GSTVKNSMVC AGGDGVRSGC QGDSGGPLHC LVNGQYAVHG VTSFVSRLGC 

       250        260 
NVTRKPTVFT RVSAYISWIN NVIASN
P00772 in FASTA format

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View entry in raw text format (no links)
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