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[1]
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NUCLEOTIDE SEQUENCE [MRNA].
PubMed=6548955 [NCBI, ExPASy, EBI, Israel, Japan]
Ullrich A.,
Gray A.,
Wood W.I.,
Hayflick J.,
Seeburg P.H.;
"Isolation of a cDNA clone coding for the gamma-subunit of mouse nerve growth factor using a high-stringency selection procedure.";
DNA 3:387-392(1984).
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[2]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=3848399 [NCBI, ExPASy, EBI, Israel, Japan]
Evans B.A.,
Richards R.I.;
"Genes for the alpha and gamma subunits of mouse nerve growth factor are contiguous.";
EMBO J. 4:133-138(1985).
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[3]
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PROTEIN SEQUENCE OF 25-261.
TISSUE=Submandibular gland;
PubMed=7263706 [NCBI, ExPASy, EBI, Israel, Japan]
Thomas K.A.,
Baglan N.C.,
Bradshaw R.A.;
"The amino acid sequence of the gamma-subunit of mouse submaxillary gland 7 S nerve growth factor.";
J. Biol. Chem. 256:9156-9166(1981).
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[4]
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X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX.
STRAIN=Swiss Webster;
TISSUE=Submandibular gland;
DOI=10.1016/S0969-2126(97)00280-3; PubMed=9351801 [NCBI, ExPASy, EBI, Israel, Japan]
Bax B.,
Blundell T.L.,
Murray-Rust J.,
McDonald N.Q.;
"Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins.";
Structure 5:1275-1285(1997).
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- FUNCTION: 7S NGF alpha chain stabilizes the 7S complex. The beta dimer promotes neurite growth. The gamma chain is an arginine-specific protease; it may also have plasminogen activator activity, as well as mitogenic activity for chick embryo fibroblasts.
- CATALYTIC ACTIVITY: Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
- COFACTOR: Binds 2 zinc ions per 7S complex. The zinc ions are bound at the alpha-gamma interfaces.
- SUBUNIT: 7S nerve growth factor is composed of two alpha chains, a beta dimer composed of identical chains, and two gamma chains.
- MISCELLANEOUS: This precursor is cleaved into segments to produce the active form of the gamma chain, which occurs naturally as combinations of either two or three segments held together by disulfide bonds: B1 and A, or B1, C and B2.
- SIMILARITY: Belongs to the peptidase S1 family. Kallikrein subfamily [view classification].
- SIMILARITY: Contains 1 peptidase S1 domain [view classification].
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 261 AA [This is the length of the unprocessed precursor] |
Molecular weight: 28998 Da [This is the MW of the unprocessed precursor] |
CRC64: 4870748E174AF7C8 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MWFLILFLAL SLGGIDAAPP VQSRIVGGFK CEKNSQPWHV AVYRYTQYLC GGVLLDPNWV
70 80 90 100 110 120
LTAAHCYDDN YKVWLGKNNL FKDEPSAQHR FVSKAIPHPG FNMSLMRKHI RFLEYDYSND
130 140 150 160 170 180
LMLLRLSKPA DITDTVKPIT LPTEEPKLGS TCLASGWGSI TPTKFQFTDD LYCVNLKLLP
190 200 210 220 230 240
NEDCAKAHIE KVTDAMLCAG EMDGGKDTCK GDSGGPLICD GVLQGITSWG HTPCGEPDMP
250 260
GVYTKLNKFT SWIKDTMAKN P
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P00756 in FASTA format |
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