PROTEIN SEQUENCE.
TISSUE=Venom;
PubMed=7049694 [NCBI, ExPASy, EBI, Israel, Japan]
Lind P., Eaker D.;
"Amino-acid sequence of the alpha-subunit of taipoxin, an extremely potent presynaptic neurotoxin from the Australian snake taipan (Oxyuranus s. scutellatus).";
Eur. J. Biochem. 124:441-447(1982).

Comments

FUNCTION: Taipoxin is the most potent animal toxin known. The alpha chain possesses a phospholipase activity.
CATALYTIC ACTIVITY: Phosphatidylcholine + H₂O = 1-acylglycerophosphocholine + a carboxylate.
COFACTOR: Binds 1 calcium ion per subunit (By similarity).
SUBUNIT: Contains three non-covalently bound chains (alpha, beta, and gamma), each related to phospholipase A2.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed by the venom gland.
TOXIC DOSE: LD₅₀ is 0.3 mg/kg by intravenous injection.
SIMILARITY: Belongs to the phospholipase A2 family. Group I subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

PIR

A00754; PSOXA.

3D structure databases

PDB

1LX1; Model; -; A=1-119.

[ExPASy / RCSB / EBI]

PDBsum

1LX1; -.

ModBase

P00614.

Ontologies

GO:0005576;	Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-KW).
GO:0042734;	Cellular component: presynaptic membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0009405;	Biological process: pathogenesis (inferred from electronic annotation from UniProtKB-KW).
GO:0007268;	Biological process: synaptic transmission (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR016090; Phospholipase_A2.
IPR013090; Phospholipase_A2_AS.
IPR001211; Phospholipase_A2_euk.
Graphical view of domain structure.

Gene3D

G3DSA:1.20.90.10; Phospholipase_A2; 1.

PANTHER

PTHR11716; Phospholipase_A2; 1.

Pfam

PF00068; Phospholip_A2_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00389; PHPHLIPASEA2.

ProDom

PD000303; PhospholipaseA2; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00085; PA2c; 1.
SMART graphical view of domain structure.

PROSITE

PS00119; PA2_ASP; 1.
PS00118; PA2_HIS; 1.

Phylogenomic databases

HOVERGEN

P00614; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Calcium; Direct protein sequencing; Hydrolase; Lipid degradation; Metal-binding; Neurotoxin; Presynaptic neurotoxin; Secreted; Toxin.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 119`	`119`	`Phospholipase A2, taipoxin alpha chain.`	`PRO_0000161678`
`ACT_SITE`	`48 48`		`By similarity.`
`ACT_SITE`	`93 93`		`By similarity.`
`METAL`	`28 28`		`Calcium; via carbonyl oxygen (By similarity).`
`METAL`	`30 30`		`Calcium; via carbonyl oxygen (By similarity).`
`METAL`	`32 32`		`Calcium; via carbonyl oxygen (By similarity).`
`METAL`	`49 49`		`Calcium (By similarity).`
`DISULFID`	`11 72`		`By similarity.`
`DISULFID`	`27 118`		`By similarity.`
`DISULFID`	`29 45`		`By similarity.`
`DISULFID`	`44 99`		`By similarity.`
`DISULFID`	`51 92`		`By similarity.`
`DISULFID`	`61 85`		`By similarity.`
`DISULFID`	`79 90`		`By similarity.`
`HELIX`	`2 13`	`12`
`HELIX`	`19 22`	`4`
`STRAND`	`23 25`	`3`
`TURN`	`26 28`	`3`
`STRAND`	`29 31`	`3`
`HELIX`	`40 56`	`17`
`STRAND`	`70 72`	`3`
`STRAND`	`77 79`	`3`
`STRAND`	`81 83`	`3`
`HELIX`	`84 102`	`19`
`HELIX`	`107 109`	`3`
`HELIX`	`114 117`	`4`

Sequence information

Length: 119 AA [This is the length of the unprocessed precursor]

Molecular weight: 13829 Da [This is the MW of the unprocessed precursor]

CRC64: 7FAFD5DD61105C03 [This is a checksum on the sequence]

        10         20         30         40         50         60 
NLLQFGFMIR CANRRSRPVW HYMDYGCYCG KGGSGTPVDD LDRCCQVHDE CYGEAVRRFG 

        70         80         90        100        110 
CAPYWTLYSW KCYGKAPTCN TKTRCQRFVC RCDAKAAECF ARSPYQNSNW NINTKARCR

P00614 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools