[1]	PROTEIN SEQUENCE. TISSUE=Venom; PubMed=1002692 [NCBI, ExPASy, EBI, Israel, Japan] Halpert J., Eaker D.; "Isolation and amino acid sequence of a neurotoxic phospholipase A from the venom of the Australian tiger snake Notechis scutatus scutatus."; J. Biol. Chem. 251:7343-7347(1976).
[2]	X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS). TISSUE=Venom; DOI=10.1016/S0041-0101(97)00051-2; PubMed=9604284 [NCBI, ExPASy, EBI, Israel, Japan] Carredano E., Westerlund B., Persson B., Saarinen M., Ramaswamy S., Eaker D., Eklund H.; "The three-dimensional structures of two toxins from snake venom throw light on the anticoagulant and neurotoxic sites of phospholipase A2."; Toxicon 36:75-92(1998).

Comments

FUNCTION: PA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Inhibits neuromuscular transmission by blocking acetylcholine release from the nerve termini. Acts presynaptically. Notechis II-5 is less toxic than Notexin but has a higher specific phospholipase activity.
CATALYTIC ACTIVITY: Phosphatidylcholine + H₂O = 1-acylglycerophosphocholine + a carboxylate.
COFACTOR: Binds 1 calcium ion per subunit.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed by the venom gland.
TOXIC DOSE: LD₅₀ is 0.045 mg/kg by intravenous injection.
SIMILARITY: Belongs to the phospholipase A2 family. Group I subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

PIR

A00750; PSNOA5.

3D structure databases

PDB

2NOT; X-ray; 3.00 A; A/B=1-119.

[ExPASy / RCSB / EBI]

PDBsum

2NOT; -.

ModBase

P00609.

Ontologies

GO:0005576;	Cellular component: extracellular region (inferred from electronic annotation from UniProtKB-KW).
GO:0042734;	Cellular component: presynaptic membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0009405;	Biological process: pathogenesis (inferred from electronic annotation from UniProtKB-KW).
GO:0007268;	Biological process: synaptic transmission (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR016090; Phospholipase_A2.
IPR013090; Phospholipase_A2_AS.
IPR001211; Phospholipase_A2_euk.
Graphical view of domain structure.

Gene3D

G3DSA:1.20.90.10; Phospholipase_A2; 1.

PANTHER

PTHR11716; Phospholipase_A2; 1.

Pfam

PF00068; Phospholip_A2_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00389; PHPHLIPASEA2.

ProDom

PD000303; PhospholipaseA2; 1.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00085; PA2c; 1.
SMART graphical view of domain structure.

PROSITE

PS00119; PA2_ASP; 1.
PS00118; PA2_HIS; 1.

Phylogenomic databases

HOVERGEN

P00609; -.

Other

LinkHub

P00609; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Calcium; Direct protein sequencing; Hydrolase; Lipid degradation; Metal-binding; Neurotoxin; Presynaptic neurotoxin; Secreted; Toxin.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 119`	`119`	`Phospholipase A2.`	`PRO_0000161676`
`ACT_SITE`	`48 48`
`ACT_SITE`	`93 93`
`METAL`	`28 28`		`Calcium; via carbonyl oxygen.`
`METAL`	`30 30`		`Calcium; via carbonyl oxygen.`
`METAL`	`32 32`		`Calcium; via carbonyl oxygen.`
`METAL`	`49 49`		`Calcium.`
`DISULFID`	`11 71`
`DISULFID`	`27 118`
`DISULFID`	`29 45`
`DISULFID`	`44 99`
`DISULFID`	`51 92`
`DISULFID`	`60 85`
`DISULFID`	`78 90`
`HELIX`	`2 13`	`12`
`HELIX`	`19 22`	`4`
`TURN`	`26 30`	`5`
`HELIX`	`40 57`	`18`
`TURN`	`62 64`	`3`
`STRAND`	`69 72`	`4`
`STRAND`	`75 78`	`4`
`STRAND`	`81 83`	`3`
`HELIX`	`84 102`	`19`
`HELIX`	`107 109`	`3`
`HELIX`	`114 117`	`4`

Sequence information

Length: 119 AA [This is the length of the unprocessed precursor]

Molecular weight: 13676 Da [This is the MW of the unprocessed precursor]

CRC64: 032C9EEBF62BEE26 [This is a checksum on the sequence]

        10         20         30         40         50         60 
NLVQFSYLIQ CANHGRRPTR HYMDYGCYCG WGGSGTPVDE LDRCCKIHDD CYSDAEKKGC 

        70         80         90        100        110 
SPKMSAYDYY CGENGPYCRN IKKKCLRFVC DCDVEAAFCF AKAPYNNANW NIDTKKRCQ

P00609 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools