NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(84)90165-3; PubMed=6092231 [NCBI, ExPASy, EBI, Israel, Japan]
Buhk H.-J., Behrens B., Tailor R., Wilke K., Prada J.J., Gunthert U., Noyer-Weidner M., Jentsch S., Trautner T.A.;
"Restriction and modification in Bacillus subtilis: nucleotide sequence, functional organization and product of the DNA methyltransferase gene of bacteriophage SPR.";
Gene 29:51-61(1984).

[2]

NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1093/nar/12.23.9039; PubMed=6096817 [NCBI, ExPASy, EBI, Israel, Japan]
Posfai G., Baldauf F., Erdei S., Posfai J., Venetianer P., Kiss A.;
"Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR.";
Nucleic Acids Res. 12:9039-9049(1984).

Comments

FUNCTION: This enzyme methylates the 5'cytosine in the sequence GGCC and both cytosines in the sequence CCGG.
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
SUBUNIT: Monomer.
SIMILARITY: Belongs to the C5-methyltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

K02124; AAA32604.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A91516; CTBPSR.

3D structure databases

HSSP

P05102; 6MHT. [HSSP ENTRY / PDB]

ModBase

P00476.

Protein family/group databases

REBASE

2834; M.SPRI.

Ontologies

GO:0003886;	Molecular function: DNA (cytosine-5-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0009307;	Biological process: DNA restriction-modification system (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001525; C5_DNA_meth.
Graphical view of domain structure.

PANTHER

PTHR10629; C5_DNA_meth; 1.

Pfam

PF00145; DNA_methylase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00105; C5METTRFRASE.

TIGRFAMs

TIGR00675; dcm; 1.

PROSITE

PS00094; C5_MTASE_1; 1.
PS00095; C5_MTASE_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Methyltransferase; Restriction system; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 439`	`439`	`Modification methylase SPRI.`	`PRO_0000087868`
`ACT_SITE`	`78 78`		`By similarity.`
`CONFLICT`	`413 413`		`T -> A (in Ref. 2; AAA32604).`
`CONFLICT`	`434 434`		`I -> V (in Ref. 2; AAA32604).`

Sequence information

Length: 439 AA [This is the length of the unprocessed precursor]

Molecular weight: 49881 Da [This is the MW of the unprocessed precursor]

CRC64: 407DCF178D32D5BE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGKLRVMSLF SGIGAFEAAL RNIGVGYELV GFSEIDKYAV KSFCAIHNVD EQLNFGDVSK 

        70         80         90        100        110        120 
IDKKKLPEFD LLVGGSPCQS FSVAGHRKGF EDTRGTLFFQ YVETLKEKQP KFFVFENVKG 

       130        140        150        160        170        180 
LINHDKGNTL NVMAEAFSEV GYRIDLELLN SKFFNVPQNR ERLYIIGIRE DLIKNEEWSL 

       190        200        210        220        230        240 
DFKRKDILQK GKQRLVELDI KSFNFRWTAQ SAATKRLKDL LEEYVDEKYY LNEDKTNSLI 

       250        260        270        280        290        300 
KELSTSRLNE NLTVEQVGNI NPSGNGMNGN VYNSSGLSPT ITTNKGEGLK IAVEYSRKSG 

       310        320        330        340        350        360 
LGRELAVSHT LSASDWRGLN RNQKQNAVVE VRPVLTPERG EKRQNGRRFK DDGEPAFTVN 

       370        380        390        400        410        420 
TIDRHGVAVG EYPKYRIRRL TPLECFRLQA FDDEDFEKAF AAGISNSQLY KQTGNSITVT 

       430 
VLESIFKELI HTYINKESE

P00476 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools