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UniProtKB/Swiss-Prot entry P00471


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name TYSY_BPT4
Primary accession number P00471
Secondary accession numbers None
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on July 21, 1986 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 68)
Name and origin of the protein
Protein name Thymidylate synthase
Synonyms TSase
TS
EC 2.1.1.45
Gene name
Name: TD
From
Enterobacteria phage T4 (Bacteriophage T4) [TaxID: 10665] 
Taxonomy Viruses; dsDNA viruses, no RNA stage; Caudovirales; Myoviridae; T4-like viruses.
Virus host Escherichia coli [TaxID: 562]
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=6328492 [NCBI, ExPASy, EBI, Israel, Japan]
Chu F.K., Maley G.F., Maley F., Belfort M.;
"Intervening sequence in the thymidylate synthase gene of bacteriophage T4.";
Proc. Natl. Acad. Sci. U.S.A. 81:3049-3053(1984).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1128/MMBR.67.1.86-156.2003; PubMed=12626685 [NCBI, ExPASy, EBI, Israel, Japan]
Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
"Bacteriophage T4 genome.";
Microbiol. Mol. Biol. Rev. 67:86-156(2003).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42.
PubMed=6327673 [NCBI, ExPASy, EBI, Israel, Japan]
Purohit S., Mathews C.K.;
"Nucleotide sequence reveals overlap between T4 phage genes encoding dihydrofolate reductase and thymidylate synthase.";
J. Biol. Chem. 259:6261-6266(1984).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 179-188.
DOI=10.1016/0092-8674(86)90379-X; PubMed=3698096 [NCBI, ExPASy, EBI, Israel, Japan]
Chu F.K., Maley G.F., West D.K., Belfort M., Maley F.;
"Characterization of the intron in the phage T4 thymidylate synthase gene and evidence for its self-excision from the primary transcript.";
Cell 45:157-166(1986).
[5]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 251-286.
PubMed=2846540 [NCBI, ExPASy, EBI, Israel, Japan]
Tseng M.J., Hilfinger J.M., Walsh A., Greenberg G.R.;
"Total sequence, flanking regions, and transcripts of bacteriophage T4 nrdA gene, coding for alpha chain of ribonucleoside diphosphate reductase.";
J. Biol. Chem. 263:16242-16251(1988).
[6]
X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS).
DOI=10.1021/bi00255a028; PubMed=7803410 [NCBI, ExPASy, EBI, Israel, Japan]
Finer-Moore J.S., Maley G.F., Maley F., Montfort W.R., Stroud R.M.;
"Crystal structure of thymidylate synthase from T4 phage: component of a deoxynucleoside triphosphate-synthesizing complex.";
Biochemistry 33:15459-15468(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
K02035; AAA32492.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K01804; AAA32492.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF158101; AAD42662.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M12742; AAC12816.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
J03968; AAA32525.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A00550; SYBPT4.
RefSeq NP_049848.1; -.
3D structure databases
PDB
1TIS; X-ray; 2.70 A; A=1-286.[ExPASy / RCSB / EBI]
PDBsum 1TIS; -.
ModBase P00471.
Family and domain databases
InterPro IPR000398; Thymidylat_synth_C.
Graphical view of domain structure.
Gene3D G3DSA:3.30.572.10; Thymidylat_synth_C; 1.
PANTHER PTHR11549:SF2; Thymidylat_synth_C; 1.
Pfam PF00303; Thymidylat_synt; 1.
Pfam graphical view of domain structure.
PRINTS PR00108; THYMDSNTHASE.
ProDom PD001180; Thymidylat_synth; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR03284; thym_sym; 1.
PROSITE PS00091; THYMIDYLATE_SYNTHASE; 1.
BLOCKS P00471.
ProtoNet P00471.
Genome annotation databases
GeneID 1258770; -.
GenomeReviews AF158101_GR; T4p238.
Other
LinkHub P00471; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Methyltransferase; Nucleotide biosynthesis; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   286  286     Thymidylate synthase. PRO_0000141061
ACT_SITE   156   156         
HELIX   3    12  10      
STRAND   26    30  5      
STRAND   33    37  5      
HELIX   38    40  3      
HELIX   53    63  11      
HELIX   69    77  9      
STRAND   80    83  4      
HELIX   88    91  4      
TURN   92    95  4      
HELIX   96    98  3      
HELIX   109   114  6      
HELIX   121   131  11      
HELIX   145   150  6      
STRAND   156   164  9      
STRAND   166   179  14      
TURN   180   183  4      
HELIX   184   202  19      
STRAND   205   221  17      
TURN   224   226  3      
HELIX   227   229  3      
HELIX   247   251  5      
HELIX   254   262  9      
HELIX   266   268  3      
Sequence information
Length: 286 AA [This is the length of the unprocessed precursor] Molecular weight: 33073 Da [This is the MW of the unprocessed precursor] CRC64: 9F60863145F5219D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKQYQDLIKD IFENGYETDD RTGTGTIALF GSKLRWDLTK GFPAVTTKKL AWKACIAELI 

        70         80         90        100        110        120 
WFLSGSTNVN DLRLIQHDSL IQGKTVWDEN YENQAKDLGY HSGELGPIYG KQWRDFGGVD 

       130        140        150        160        170        180 
QIIEVIDRIK KLPNDRRQIV SAWNPAELKY MALPPCHMFY QFNVRNGYLD LQWYQRSVDV 

       190        200        210        220        230        240 
FLGLPFNIAS YATLVHIVAK MCNLIPGDLI FSGGNTHIYM NHVEQCKEIL RREPKELCEL 

       250        260        270        280 
VISGLPYKFR YLSTKEQLKY VLKLRPKDFV LNNYVSHPPI KGKMAV 

P00471 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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