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UniProtKB/Swiss-Prot entry P00440


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name TYRO_NEUCR
Primary accession number P00440
Secondary accession numbers Q6MGJ7 Q7RVL7
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on December 4, 2007 (Sequence version 5)
Annotations were last modified on    November 4, 2008 (Entry version 70)
Name and origin of the protein
Protein name Tyrosinase [Precursor]
Synonyms EC 1.14.18.1
Monophenol monooxygenase
Gene name
Name: T
ORFNames: 90C4.150, NCU00776
From
Neurospora crassa [TaxID: 5141] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=Oak Ridge, and TS;
PubMed=2529259 [NCBI, ExPASy, EBI, Israel, Japan]
Kupper U., Niedermann D.M., Travaglini G., Lerch K.;
"Isolation and characterization of the tyrosinase gene from Neurospora crassa.";
J. Biol. Chem. 264:17250-17258(1989).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
DOI=10.1093/nar/gkg293; PubMed=12655011 [NCBI, ExPASy, EBI, Israel, Japan]
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence.";
Nucleic Acids Res. 31:1944-1954(2003).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
DOI=10.1038/nature01554; PubMed=12712197 [NCBI, ExPASy, EBI, Israel, Japan]
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
"The genome sequence of the filamentous fungus Neurospora crassa.";
Nature 422:859-868(2003).
[4]
PROTEIN SEQUENCE OF 2-408.
STRAIN=TL;
PubMed=6210696 [NCBI, ExPASy, EBI, Israel, Japan]
Lerch K.;
"Primary structure of tyrosinase from Neurospora crassa. II. Complete amino acid sequence and chemical structure of a tripeptide containing an unusual thioether.";
J. Biol. Chem. 257:6414-6419(1982).
[5]
PROTEIN SEQUENCE OF 2-408.
STRAIN=Sing, and TS;
PubMed=6210697 [NCBI, ExPASy, EBI, Israel, Japan]
Ruegg C., Ammer D., Lerch K.;
"Comparison of amino acid sequence and thermostability of tyrosinase from three wild type strains of Neurospora crassa.";
J. Biol. Chem. 257:6420-6426(1982).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M32843; AAA33619.1; ALT_FRAME; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M33271; AAA33618.1; ALT_FRAME; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BX842680; CAE81941.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AABX02000002; EAA35696.2; ALT_SEQ; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A34460; YRNC.
3D structure databases
ModBase P00440.
Ontologies
GO
GO:0005507; Molecular function: copper ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004503; Molecular function: monophenol monooxygenase activity (inferred from electronic annotation from EC).
GO:0006583; Biological process: melanin biosynthetic process from tyrosine (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR008922; Di-copper_centre.
IPR016216; Monophenol_mOase_fun.
IPR002227; Tyrosinase.
Graphical view of domain structure.
Gene3D G3DSA:1.10.1280.10; Di-copper_centre; 1.
Pfam PF00264; Tyrosinase; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000340; MPO_fungal; 1.
PRINTS PR00092; TYROSINASE.
PROSITE PS00497; TYROSINASE_1; 1.
PS00498; TYROSINASE_2; 1.
BLOCKS P00440.
ProtoNet P00440.
Other
DrugBank DB00548; Azelaic Acid.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Complete proteome; Copper; Direct protein sequencing; Melanin biosynthesis; Metal-binding; Monooxygenase; Oxidoreductase; Thioether bond.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   408  407     Tyrosinase. PRO_0000035895
PROPEP   409   685  277     Could be involved in enzyme activation. PRO_0000035896
METAL   67    67        Copper A (By similarity). 
METAL   97    97        Copper A (By similarity). 
METAL   106   106        Copper A (By similarity). 
METAL   278   278        Copper B (By similarity). 
METAL   282   282        Copper B (By similarity). 
METAL   307   307        Copper B (By similarity). 
MOD_RES   2     2        N-acetylserine. 
CROSSLNK   95    97        2'-(S-cysteinyl)-histidine (Cys-His). 
VARIANT   15    15  1     P -> T (in strain: Sing, TL and TS). 
VARIANT   30    30  1     D -> E (in strain: Sing). 
VARIANT   130   130  1     V -> T (in strain: Sing). 
VARIANT   202   202  1     D -> N (in strain: TL). 
VARIANT   346   347  2     KS -> QN (in strain: Sing). 
VARIANT   371   371  1     I -> T (in strain: Sing). 
VARIANT   424   424  1     K -> N (in strain: TS). 
VARIANT   450   450  1     K -> R (in strain: TS). 
VARIANT   678   678  1     G -> R (in strain: TS). 
CONFLICT   235   235        N -> D (in Ref. 4 and 5). 
Sequence information
Length: 685 AA [This is the length of the unprocessed precursor] Molecular weight: 75886 Da [This is the MW of the unprocessed precursor] CRC64: DF64B764BFF5468A [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSTDIKFAIT GVPTPPSSNG AVPLRRELRD LQQNYPEQFN LYLLGLRDFQ GLDEAKLDSY 

        70         80         90        100        110        120 
YQVAGIHGMP FKPWAGVPSD TDWSQPGSSG FGGYCTHSSI LFITWHRPYL ALYEQALYAS 

       130        140        150        160        170        180 
VQAVAQKFPV EGGLRAKYVA AAKDFRAPYF DWASQPPKGT LAFPESLSSR TIQVVDVDGK 

       190        200        210        220        230        240 
TKSINNPLHR FTFHPVNPSP GDFSAAWSRY PSTVRYPNRL TGASRDERIA PILANELASL 

       250        260        270        280        290        300 
RNNVSLLLLS YKDFDAFSYN RWDPNTNPGD FGSLEDVHNE IHDRTGGNGH MSSLEVSAFD 

       310        320        330        340        350        360 
PLFWLHHVNV DRLWSIWQDL NPNSFMTPRP APYSTFVAQE GESQSKSTPL EPFWDKSAAN 

       370        380        390        400        410        420 
FWTSEQVKDS ITFGYAYPET QKWKYSSVKE YQAAIRKSVT ALYGSNVFAN FVENVADRTP 

       430        440        450        460        470        480 
ALKKPQATGE ESKSTVSAAA AHAVELSGAK KVAEKVHNVF QHAEEKAQKP VVPVKDTKAE 

       490        500        510        520        530        540 
SSTAAGMMIG LSIKRPSKLT ASPGPIPESL KYLAPDGKYT DWIVNVRAQK HGLGQSFRVI 

       550        560        570        580        590        600 
VFLGEFNPDP ETWDDEFNCV GRVSVLGRSA ETQCGKCRKD NANGLIVSGT VPLTSALLQD 

       610        620        630        640        650        660 
IVGGELQSLK PEDVIPHLRA NLKWKVALFN GDEYNLEEVP DLKVSVASTE VTIDEEGLPH 

       670        680 
YSRQYTVYPE ITEGKPCGHG PEDHI 

P00440 in FASTA format

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