[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. PubMed=7153689 [NCBI, ExPASy, EBI, Israel, Japan] Depicker A., Stachel S., Dhaese P., Zambryski P., Goodman H.M.; "Nopaline synthase: transcript mapping and DNA sequence."; J. Mol. Appl. Genet. 1:561-573(1982).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1093/nar/11.2.369; PubMed=6298724 [NCBI, ExPASy, EBI, Israel, Japan] Bevan M., Barnes W.M., Chilton M.-D.; "Structure and transcription of the nopaline synthase gene region of T-DNA."; Nucleic Acids Res. 11:369-385(1983).

Comments

CATALYTIC ACTIVITY: N²-(D-1,3-dicarboxypropyl)-L-arginine + NADP⁺ + H₂O = L-arginine + 2-oxoglutarate + NADPH.
SUBUNIT: Homotetramer.
SIMILARITY: Belongs to the lysopine/nopaline/octopine/opine/vitopine dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

V00087; CAA23427.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A92833; DEAGNT.

3D structure databases

ModBase

P00386.

Ontologies

GO:0047829;	Molecular function: D-nopaline dehydrogenase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR013328; DHase_multihelical.
IPR016040; NAD(P)-bd.
IPR011128; NAD-dep_Gly3P_DHase_N.
IPR003421; Opine_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.
G3DSA:1.10.1040.10; Opine_DH; 1.

Pfam

PF01210; NAD_Gly3P_dh_N; 1.
PF02317; Octopine_DH; 1.
Pfam graphical view of domain structure.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Crown gall tumor; NADP; Oxidoreductase; Plasmid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 413`	`413`	`D-nopaline dehydrogenase.`	`PRO_0000179983`
`CONFLICT`	`222 222`		`C -> Y (in Ref. 2).`
`CONFLICT`	`359 359`		`N -> K (in Ref. 2).`
`CONFLICT`	`362 362`		`L -> I (in Ref. 2).`

Sequence information

Length: 413 AA [This is the length of the unprocessed precursor]

Molecular weight: 45387 Da [This is the MW of the unprocessed precursor]

CRC64: 9639D2D73C57CEF8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAITLSATSL PISAADHHPL PLTVGVLGSG HAGTALAAWF ASRHVPTALW APADHPGSIS 

        70         80         90        100        110        120 
AIKANEGVIT TEGMINGPFR VSACDDLAAV IRSSRVLIIV TRADVHDSFV NELANFNGEL 

       130        140        150        160        170        180 
ATKDIVVVCG HGFSIKYERQ LRFKRIFETD NSPITSKLSD QKKCNVNIKE MKASFGLSCF 

       190        200        210        220        230        240 
PIHRDDAGVI DLPEDTKNIF AQLFSARIIC IPPLQVLFFS NCITHAVPAV MNIGRLRDPA 

       250        260        270        280        290        300 
NSLTKRAEKW LLELDERTPR AEKGFFFYGE GSNTYVCNVQ EQIDHERRKV AAACGLRLNS 

       310        320        330        340        350        360 
LLQECNDEYD TDYETLREYC LAPSPHNVHH ACPDNMEHRY FSEELCSLED VAAIAAIANI 

       370        380        390        400        410 
ELPLTHAFIN IIHAGKGKIN PTGKSSSVIG NFSSSDLIRF GATHVFNKDE MVE

P00386 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools