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UniProtKB/Swiss-Prot entry P00382


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DYR1_ECOLX
Primary accession number P00382
Secondary accession number P13923
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on July 21, 1986 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 57)
Name and origin of the protein
Protein name Dihydrofolate reductase type 1
Synonyms EC 1.5.1.3
Dihydrofolate reductase type I
Trimethoprim resistance protein
Gene name
Name: dhfrI
From
Escherichia coli [TaxID: 562] 
Encoded on Plasmid IncI1 R483; Plasmid pLMO150; Plasmid pLMO229.
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
TRANSPOSON=Tn7;
DOI=10.1093/nar/11.15.5147; PubMed=6308574 [NCBI, ExPASy, EBI, Israel, Japan]
Fling M.E., Richards C.;
"The nucleotide sequence of the trimethoprim-resistant dihydrofolate reductase gene harbored by Tn7.";
Nucleic Acids Res. 11:5147-5158(1983).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PLASMID=IncI1 R483;
DOI=10.1093/nar/16.5.2235; PubMed=3357775 [NCBI, ExPASy, EBI, Israel, Japan]
Simonsen C.S., Walter M., Levinson A.D.;
"Expression of the plasmid-encoded type I dihydrofolate reductase gene in cultured mammalian cells: a novel selectable marker.";
Nucleic Acids Res. 16:2235-2246(1988).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PLASMID=pLMO150, and pLMO229;
PubMed=2188588 [NCBI, ExPASy, EBI, Israel, Japan]
Sundstroem L., Skoeld O.;
"The dhfrI trimethoprim resistance gene of Tn7 can be found at specific sites in other genetic surroundings.";
Antimicrob. Agents Chemother. 34:642-650(1990).
[4]
PROTEIN SEQUENCE OF 1-34.
PLASMID=IncI1 R483;
PubMed=6350298 [NCBI, ExPASy, EBI, Israel, Japan]
Novak P., Stone D., Burchall J.J.;
"R plasmid dihydrofolate reductase with a dimeric subunit structure.";
J. Biol. Chem. 258:10956-10959(1983).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X00926; CAA25445.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X17477; CAA35509.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X17478; CAA35512.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S03651; RDECD7.
RefSeq NP_065309.1; -.
YP_190212.1; -.
3D structure databases
HSSP P16184; 1DYR. [HSSP ENTRY / PDB]
ModBase P00382.
Ontologies
GO
GO:0006730; Biological process: one-carbon compound metabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0046677; Biological process: response to antibiotic (inferred from electronic annotation from UniProtKB-KW).
GO:0031427; Biological process: response to methotrexate (inferred from electronic annotation from UniProtKB-KW).
GO:0032196; Biological process: transposition (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR012259; DHFR.
IPR001796; DHFR_reg.
Graphical view of domain structure.
PANTHER PTHR11549:SF1; DHFR; 1.
Pfam PF00186; DHFR_1; 1.
Pfam graphical view of domain structure.
PRINTS PR00070; DHFR.
PROSITE PS00075; DHFR_1; 1.
PS51330; DHFR_2; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P00382.
ProtoNet P00382.
Genome annotation databases
GeneID 1238688; -.
3244885; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Antibiotic resistance; Direct protein sequencing; Methotrexate resistance; NADP; One-carbon metabolism; Oxidoreductase; Plasmid; Transposable element; Trimethoprim resistance.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom  To Length Description FTId
CHAIN   1   157  157     Dihydrofolate reductase type 1. PRO_0000186420
DOMAIN   2   156  155     DHFR. 
VARIANT   75    75  1     L -> V (in plasmid pLMO229). 
Sequence information
Length: 157 AA [This is the length of the unprocessed precursor] Molecular weight: 17575 Da [This is the MW of the unprocessed precursor] CRC64: 487F818A40E4991B [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKLSLMVAIS KNGVIGNGPD IPWSAKGEQL LFKAITYNQW LLVGRKTFES MGALPNRKYA 

        70         80         90        100        110        120 
VVTRSSFTSD NENVLIFPSI KDALTNLKKI TDHVIVSGGG EIYKSLIDQV DTLHISTIDI 

       130        140        150 
EPEGDVYFPE IPSNFRPVFT QDFASNINYS YQIWQKG 

P00382 in FASTA format

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