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UniProtKB/Swiss-Prot entry P00370

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHE4_ECOLI
Primary accession number P00370
Secondary accession number P78173
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on July 21, 1986 (Sequence version 1)
Annotations were last modified on    November 4, 2008 (Entry version 72)
Name and origin of the protein
Protein name NADP-specific glutamate dehydrogenase
Synonyms NADP-GDH
EC 1.4.1.4
Gene name
Name: gdhA
OrderedLocusNames: b1761, JW1750
From
Escherichia coli (strain K12) [TaxID: 83333] [HAMAP proteome]
Taxonomy Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; Enterobacteriaceae; Escherichia.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-36.
STRAIN=K12;
DOI=10.1093/nar/11.15.5257; PubMed=6308576 [NCBI, ExPASy, EBI, Israel, Japan]
McPherson M.J., Wootton J.C.;
"Complete nucleotide sequence of the Escherichia coli gdhA gene.";
Nucleic Acids Res. 11:5257-5266(1983).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(84)90140-9; PubMed=6373501 [NCBI, ExPASy, EBI, Israel, Japan]
Valle F., Becerril B., Chen E., Seeburg P.H., Heyneker H., Bolivar F.;
"Complete nucleotide sequence of the glutamate dehydrogenase gene from Escherichia coli K-12.";
Gene 27:193-199(1984).
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1093/dnares/3.6.363; PubMed=9097039 [NCBI, ExPASy, EBI, Israel, Japan]
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.;
"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map.";
DNA Res. 3:363-377(1996).
[4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / MG1655 / ATCC 47076;
DOI=10.1126/science.277.5331.1453; PubMed=9278503 [NCBI, ExPASy, EBI, Israel, Japan]
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.;
"The complete genome sequence of Escherichia coli K-12.";
Science 277:1453-1474(1997).
[5]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
DOI=10.1038/msb4100049; PubMed=16738553 [NCBI, ExPASy, EBI, Israel, Japan]
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.";
Mol. Syst. Biol. 2:E1-E5(2006).
[6]
 PROTEIN SEQUENCE OF 1-12.
STRAIN=K12 / EMG2;
DOI=10.1002/elps.1150180807; PubMed=9298646 [NCBI, ExPASy, EBI, Israel, Japan]
Link A.J., Robison K., Church G.M.;
"Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12.";
Electrophoresis 18:1259-1313(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J01615; AAA87979.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K02499; AAA23868.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U00096; AAC74831.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AP009048; BAA15550.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A00382; DEECEN.
RefSeq AP_002380.1; -.
NP_416275.1; -.
3D structure databases
HSSP P24295; 1AUP. [HSSP ENTRY / PDB]
ModBase P00370.
Protein-protein interaction databases
DIP DIP:9756N; -.
Enzyme and pathway databases
BioCyc EcoCyc:GDHA-MON; -.
MetaCyc:GDHA-MON; -.
2D gel databases
SWISS-2DPAGE P00370; -.
ECO2DBASE G043.6; 6TH EDITION.
G043.7; 6TH EDITION.
Organism-specific databases
EchoBASE EB0367; -.
EcoGene EG10372; gdhA.
Ontologies
GO
GO:0004354; Molecular function: glutamate dehydrogenase (NADP+) activity (inferred from electronic annotation from EC).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR006095; Glu/Leu/Phe/Val_DHase.
IPR006096; Glu/Leu/Phe/Val_DHase_C.
IPR006097; Glu/Leu/Phe/Val_DHase_dimer.
IPR014362; Glu_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11606:SF2; GLFV_DH; 1.
Pfam PF00208; ELFV_dehydrog; 1.
PF02812; ELFV_dehydrog_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000185; Glu_DH; 1.
PRINTS PR00082; GLFDHDRGNASE.
PROSITE PS00074; GLFV_DEHYDROGENASE; 1.
BLOCKS P00370.
ProtoNet P00370.
Genome annotation databases
GeneID 946802; -.
GenomeReviews U00096_GR; b1761.
AP009048_GR; JW1750.
KEGG ecj:JW1750; -.
eco:b1761; -.
Phylogenomic databases
HOGENOM P00370; -.
Genome annotation databases
CMR P00370; b1761.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Direct protein sequencing; NADP; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   447  447     NADP-specific glutamate dehydrogenase. PRO_0000182769
ACT_SITE   128   128        By similarity. 
CONFLICT   385   385        A -> P (in Ref. 2; AAA23868). 
Sequence information
Length: 447 AA [This is the length of the unprocessed precursor] Molecular weight: 48581 Da [This is the MW of the unprocessed precursor] CRC64: 7CB861D282C533C6 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MDQTYSLESF LNHVQKRDPN QTEFAQAVRE VMTTLWPFLE QNPKYRQMSL LERLVEPERV 

        70         80         90        100        110        120 
IQFRVVWVDD RNQIQVNRAW RVQFSSAIGP YKGGMRFHPS VNLSILKFLG FEQTFKNALT 

       130        140        150        160        170        180 
TLPMGGGKGG SDFDPKGKSE GEVMRFCQAL MTELYRHLGA DTDVPAGDIG VGGREVGFMA 

       190        200        210        220        230        240 
GMMKKLSNNT ACVFTGKGLS FGGSLIRPEA TGYGLVYFTE AMLKRHGMGF EGMRVSVSGS 

       250        260        270        280        290        300 
GNVAQYAIEK AMEFGARVIT ASDSSGTVVD ESGFTKEKLA RLIEIKASRD GRVADYAKEF 

       310        320        330        340        350        360 
GLVYLEGQQP WSLPVDIALP CATQNELDVD AAHQLIANGV KAVAEGANMP TTIEATELFQ 

       370        380        390        400        410        420 
QAGVLFAPGK AANAGGVATS GLEMAQNAAR LGWKAEKVDA RLHHIMLDIH HACVEHGGEG 

       430        440 
EQTNYVQGAN IAGFVKVADA MLAQGVI
P00370 in FASTA format

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