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UniProtKB/Swiss-Prot entry P00346

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MDHM_PIG
Primary accession number P00346
Secondary accession number Q95308
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on November 1, 1997 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 79)
Name and origin of the protein
Protein name Malate dehydrogenase, mitochondrial [Precursor]
Synonym EC 1.1.1.37
Gene name
Name: MDH2
From
Sus scrofa (Pig) [TaxID: 9823] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Suina; Suidae; Sus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1-40.
TISSUE=Small intestine;
DOI=10.1007/s003359900153; PubMed=8672129 [NCBI, ExPASy, EBI, Israel, Japan]
Winteroe A.K., Fredholm M., Davies W.;
"Evaluation and characterization of a porcine small intestine cDNA library: analysis of 839 clones.";
Mamm. Genome 7:509-517(1996).
[2]
 PROTEIN SEQUENCE OF 25-338.
PubMed=6959107 [NCBI, ExPASy, EBI, Israel, Japan]
Birktoft J.J., Fernley R.T., Bradshaw R.A., Banaszak L.J.;
"Amino acid sequence homology among the 2-hydroxy acid dehydrogenases: mitochondrial and cytoplasmic malate dehydrogenases form a homologous system with lactate dehydrogenase.";
Proc. Natl. Acad. Sci. U.S.A. 79:6166-6170(1982).
[3]
 NUCLEOTIDE SEQUENCE [MRNA] OF 41-338.
DOI=10.1021/bi00383a017; PubMed=3038184 [NCBI, ExPASy, EBI, Israel, Japan]
Joh T., Takeshima H., Tsuzuki T., Shimada K., Tanase S., Morino Y.;
"Cloning and sequence analysis of cDNAs encoding mammalian mitochondrial malate dehydrogenase.";
Biochemistry 26:2515-2520(1987).
[4]
 X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) IN COMPLEX WITH SUBSTRATE ANALOG, AND SUBUNIT.
TISSUE=Heart;
DOI=10.1021/bi00174a014; PubMed=8117664 [NCBI, ExPASy, EBI, Israel, Japan]
Gleason W.B., Fu Z., Birktoft J.J., Banaszak L.J.;
"Refined crystal structure of mitochondrial malate dehydrogenase from porcine heart and the consensus structure for dicarboxylic acid oxidoreductases.";
Biochemistry 33:2078-2088(1994).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z81157; CAB03545.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M16427; AAA31071.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A00355; DEPGMM.
UniGene Ssc.12417
3D structure databases
PDB
1MLD; X-ray; 1.83 A; A/B/C/D=25-338.[ExPASy / RCSB / EBI]
PDBsum 1MLD; -.
ModBase P00346.
Ontologies
GO
GO:0005759; Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.
Family and domain databases
InterPro IPR001557; L-lactate/malate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR001252; Malate_DHase_AS.
IPR010097; Malate_DHase_NAD-dep_euk_g_bac.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR11540:SF1; MDH_euk_g_bac; 1.
Pfam PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000102; Lac_mal_DH; 1.
TIGRFAMs TIGR01772; MDH_euk_gproteo; 1.
PROSITE PS00068; MDH; 1.
BLOCKS P00346.
Phylogenomic databases
HOVERGEN P00346; -.
Other
LinkHub P00346; -.
ProtoNet P00346.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Acetylation; Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Phosphoprotein; Transit peptide; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    24  24     Mitochondrion. 
CHAIN   25   338  314     Malate dehydrogenase, mitochondrial. PRO_0000018630
NP_BIND   31    37  7     NAD (By similarity). 
NP_BIND   140   142  3     NAD (By similarity). 
ACT_SITE   200   200        Proton acceptor. 
BINDING   57    57        NAD (By similarity). 
BINDING   104   104        Substrate. 
BINDING   110   110        Substrate. 
BINDING   117   117        NAD (By similarity). 
BINDING   142   142        Substrate. 
BINDING   176   176        Substrate. 
BINDING   251   251        NAD (By similarity). 
MOD_RES   56    56        Phosphotyrosine (By similarity). 
MOD_RES   157   157        N6-acetyllysine (By similarity). 
MOD_RES   239   239        N6-acetyllysine (By similarity). 
MOD_RES   314   314        N6-acetyllysine (By similarity). 
CONFLICT   120   120        I -> M (in Ref. 3; AAA31071). 
CONFLICT   126   126        A -> V (in Ref. 3; AAA31071). 
CONFLICT   150   150        I -> M (in Ref. 3; AAA31071). 
CONFLICT   304   304        G -> R (in Ref. 3; AAA31071). 
CONFLICT   337   337        M -> T (in Ref. 3; AAA31071). 
STRAND   26    31  6      
HELIX   37    45  9      
STRAND   51    60  10      
HELIX   61    68  8      
STRAND   71    74  4      
STRAND   76    82  7      
HELIX   83    85  3      
HELIX   86    90  5      
STRAND   94    98  5      
HELIX   110   113  4      
HELIX   114   131  18      
STRAND   135   139  5      
HELIX   144   157  14      
STRAND   165   168  4      
HELIX   171   184  14      
HELIX   189   191  3      
STRAND   196   198  3      
HELIX   202   204  3      
STRAND   205   207  3      
HELIX   209   211  3      
HELIX   220   241  22      
HELIX   249   267  19      
STRAND   273   279  7      
STRAND   282   295  14      
STRAND   298   302  5      
HELIX   310   334  25      
Sequence information
Length: 338 AA [This is the length of the unprocessed precursor] Molecular weight: 35596 Da [This is the MW of the unprocessed precursor] CRC64: B0435E87383D0B1E [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MLSALARPAG AALRRSFSTS XQNNAKVAVL GASGGIGQPL SLLLKNSPLV SRLTLYDIAH 

        70         80         90        100        110        120 
TPGVAADLSH IETRATVKGY LGPEQLPDCL KGCDVVVIPA GVPRKPGMTR DDLFNTNATI 

       130        140        150        160        170        180 
VATLTAACAQ HCPDAMICII SNPVNSTIPI TAEVFKKHGV YNPNKIFGVT TLDIVRANAF 

       190        200        210        220        230        240 
VAELKGLDPA RVSVPVIGGH AGKTIIPLIS QCTPKVDFPQ DQLSTLTGRI QEAGTEVVKA 

       250        260        270        280        290        300 
KAGAGSATLS MAYAGARFVF SLVDAMNGKE GVVECSFVKS QETDCPYFST PLLLGKKGIE 

       310        320        330 
KNLGIGKISP FEEKMIAEAI PELKASIKKG EEFVKNMK
P00346 in FASTA format

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