ID   LDHA_CHICK              Reviewed;         332 AA.
AC   P00340;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   04-NOV-2008, entry version 67.
DE   RecName: Full=L-lactate dehydrogenase A chain;
DE            Short=LDH-A;
DE            EC=1.1.1.27;
GN   Name=LDHA;
OS   Gallus gallus (Chicken).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archosauria; Dinosauria; Saurischia; Theropoda; Coelurosauria; Aves;
OC   Neognathae; Galliformes; Phasianidae; Phasianinae; Gallus.
OX   NCBI_TaxID=9031;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=91057138; PubMed=2243792; DOI=10.1093/nar/18.21.6432;
RA   Hirota Y., Katsumata A., Takeya T.;
RT   "Nucleotide and deduced amino acid sequences of chicken lactate
RT   dehydrogenase-A.";
RL   Nucleic Acids Res. 18:6432-6432(1990).
RN   [2]
RP   PROTEIN SEQUENCE OF 2-332.
RA   Torff H.-J., Becker D., Schwarzwalder J.;
RL   (In) Sund H. (eds.);
RL   Pyridine nucleotide dependent dehydrogenases, pp.31-42, Walter de
RL   Gruyter, Berlin (1977).
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; X53828; CAA37824.1; -; mRNA.
DR   PIR; A00349; DECHLM.
DR   PIR; S12151; S12151.
DR   RefSeq; NP_990615.1; -.
DR   UniGene; Gga.4398; -.
DR   HSSP; P00338; 1I10.
DR   SMR; P00340; 2-332.
DR   Ensembl; ENSGALG00000006300; Gallus gallus.
DR   GeneID; 396221; -.
DR   KEGG; gga:396221; -.
DR   HOGENOM; P00340; -.
DR   HOVERGEN; P00340; -.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001557; L-lactate/malate_DHase.
DR   InterPro; IPR011304; L-lactate_DHase.
DR   InterPro; IPR001236; Lactate/malate_DHase.
DR   InterPro; IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Glycolysis; NAD; Oxidoreductase.
FT   INIT_MET      1      1       Removed.
FT   CHAIN         2    332       L-lactate dehydrogenase A chain.
FT                                /FTId=PRO_0000168420.
FT   NP_BIND      29     57       NAD (By similarity).
FT   ACT_SITE    193    193       Proton acceptor (By similarity).
FT   BINDING      99     99       NAD (By similarity).
FT   BINDING     106    106       Substrate (By similarity).
FT   BINDING     138    138       NAD or substrate (By similarity).
FT   BINDING     169    169       Substrate (By similarity).
FT   BINDING     248    248       Substrate (By similarity).
FT   CONFLICT     63     63       L -> M (in Ref. 2; AA sequence).
FT   CONFLICT     78     78       I -> T (in Ref. 2; AA sequence).
FT   CONFLICT    192    192       E -> Q (in Ref. 2; AA sequence).
SQ   SEQUENCE   332 AA;  36514 MW;  5ED8279CFAD7B62B CRC64;
     MSLKDHLIHN VHKEEHAHAH NKISVVGVGA VGMACAISIL MKDLADELTL VDVVEDKLKG
     EMLDLQHGSL FLKTPKIISG KDYSVTAHSK LVIVTAGARQ QEGESRLNLV QRNVNIFKFI
     IPNVVKYSPD CKLLIVSNPV DILTYVAWKI SGFPKHRVIG SGCNLDSARF RHLMGERLGI
     HPLSCHGWIV GEHGDSSVPV WSGVNVAGVS LKALHPDMGT DADKEHWKEV HKQVVDSAYE
     VIKLKGYTSW AIGLSVADLA ETIMKNLRRV HPISTAVKGM HGIKDDVFLS VPCVLGSSGI
     TDVVKMILKP DEEEKIKKSA DTLWGIQKEL QF
//