[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], PROTEIN SEQUENCE OF 1-129, AND CHARACTERIZATION. TISSUE=Lung; DOI=10.1074/jbc.273.51.34502; PubMed=9852119 [NCBI, ExPASy, EBI, Israel, Japan] Thompson M.A., Weinshilboum R.M.; "Rabbit lung indolethylamine N-methyltransferase. cDNA and gene cloning and characterization."; J. Biol. Chem. 273:34502-34510(1998).
[2]	CHARACTERIZATION. TISSUE=Lung; DOI=10.1021/bi00535a054; PubMed=7074100 [NCBI, ExPASy, EBI, Israel, Japan] Irace G., Colonna G., Camardella M., Della Pietra G., Porta R.; "Purification and molecular properties of rabbit lung indolamine N-methyltransferase."; Biochemistry 21:1464-1470(1982).

Comments

FUNCTION: Catalyzes the N-methylation of tryptamine and structurally related compounds (Potential).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + an amine = S-adenosyl-L-homocysteine + a methylated amine.
SUBUNIT: Monomer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, also detected in liver and at very low levels in brain.
SIMILARITY: Belongs to the NNMT/PNMT/TEMT family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF077828; AAC97492.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF077827; AAC97492.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF077826; AAC97491.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001075512.1; -.

UniGene

Ocu.2318

3D structure databases

HSSP

P11086; 1HNN. [HSSP ENTRY / PDB]

SMR

O97972; 5-261.

ModBase

O97972.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0030748;	Molecular function: amine N-methyltransferase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR000940; NNMT_TEMT_trans.
Graphical view of domain structure.

PANTHER

PTHR10867; NNMT_TEMT_trans; 1.

Pfam

PF01234; NNMT_PNMT_TEMT; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000384; PNMTase; 1.

PROSITE

PS01100; NNMT_PNMT_TEMT; 1.

Genome annotation databases

GeneID

100008695; -.

Phylogenomic databases

HOVERGEN

O97972; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Direct protein sequencing; Methyltransferase; S-adenosyl-L-methionine; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 263`	`263`	`Indolethylamine N-methyltransferase.`	`PRO_0000159715`
`REGION`	`63 64`	`2`	`S-adenosyl-L-methionine binding (By similarity).`
`REGION`	`142 143`	`2`	`S-adenosyl-L-methionine binding (By similarity).`
`BINDING`	`20 20`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`25 25`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`69 69`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`85 85`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`90 90`		`S-adenosyl-L-methionine (By similarity).`
`BINDING`	`163 163`		`S-adenosyl-L-methionine; via carbonyl oxygen (By similarity).`

Sequence information

Length: 263 AA [This is the length of the unprocessed precursor]

Molecular weight: 28955 Da [This is the MW of the unprocessed precursor]

CRC64: 42F368414BE8B459 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEGGFTGGDE YQKHFLPRDY LNTYYSFQSG PSPEAEMLKF NLECLHKTFG PGGLQGDTLI 

        70         80         90        100        110        120 
DIGSGPTIYQ VLAACESFKD ITLSDFTDRN REELAKWLKK EPGAYDWTPA LKFACELEGN 

       130        140        150        160        170        180 
SGRWQEKAEK LRATVKRVLK CDANLSNPLT PVVLPPADCV LTLLAMECAC CSLDAYRAAL 

       190        200        210        220        230        240 
RNLASLLKPG GHLVTTVTLQ LSSYMVGERE FSCVALEKEE VEQAVLDAGF DIEQLLYSPQ 

       250        260 
SYSASTAPNR GVCFLVARKK PGS

O97972 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools