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UniProtKB/Swiss-Prot entry O95573


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ACSL3_HUMAN
Primary accession number O95573
Secondary accession numbers Q60I92 Q8IUM9
Integrated into Swiss-Prot on April 27, 2001
Sequence was last modified on October 3, 2006 (Sequence version 3)
Annotations were last modified on    June 16, 2009 (Entry version 80)
Name and origin of the protein
Protein name Long-chain-fatty-acid--CoA ligase 3
Synonyms EC 6.2.1.3
Long-chain acyl-CoA synthetase 3
LACS 3
Gene name
Name: ACSL3
Synonyms: ACS3, FACL3, LACS3
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT SER-551.
TISSUE=Placenta;
DOI=10.1006/geno.1997.4710; PubMed=9177793 [NCBI, ExPASy, EBI, Israel, Japan]
Minekura H., Fujino T., Kang M.-J., Fujita T., Endo Y., Yamamoto T.T.;
"Human acyl-coenzyme A synthetase 3 cDNA and localization of its gene (ACS3) to chromosome band 2q34-q35.";
Genomics 42:180-181(1997).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], AND VARIANT SER-551.
DOI=10.1016/S0378-1119(01)00714-4; PubMed=11707336 [NCBI, ExPASy, EBI, Israel, Japan]
Minekura H., Kang M.-J., Inagaki Y., Suzuki H., Sato H., Fujino T., Yamamoto T.T.;
"Genomic organization and transcription units of the human acyl-CoA synthetase 3 gene.";
Gene 278:185-192(2001).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Pancreas;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-593, AND MASS SPECTROMETRY.
TISSUE=Epithelium;
DOI=10.1073/pnas.0404720101; PubMed=15302935 [NCBI, ExPASy, EBI, Israel, Japan]
Beausoleil S.A., Jedrychowski M., Schwartz D., Elias J.E., Villen J., Li J., Cohn M.A., Cantley L.C., Gygi S.P.;
"Large-scale characterization of HeLa cell nuclear phosphoproteins.";
Proc. Natl. Acad. Sci. U.S.A. 101:12130-12135(2004).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-683 AND THR-688, AND MASS SPECTROMETRY.
DOI=10.1016/j.molcel.2008.07.007; PubMed=18691976 [NCBI, ExPASy, EBI, Israel, Japan]
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.;
"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.";
Mol. Cell 31:438-448(2008).
[6]
IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
Colinge J., Superti-Furga G., Bennett K.L.;
Submitted (OCT-2008) to UniProtKB.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
D89053; BAA37142.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB061712; BAB72074.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB061436; BAB72139.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC041692; AAH41692.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00031397; -.
RefSeq NP_004448.2; -.
NP_976251.1; -.
UniGene Hs.655772
3D structure databases
ModBase O95573.
Protein-protein interaction databases
IntAct O95573; 1.
PTM databases
PhosphoSite O95573; -.
Enzyme and pathway databases
BRENDA 6.2.1.3; 247.
Organism-specific databases
GeneCards GC02P223433; -.
HGNC HGNC:3570; ACSL3.
GenAtlas ACSL3.
HPA HPA011315; -.
MIM 602371; gene. [NCBI / EBI]
PharmGKB PA27967; -.
Gene expression databases
ArrayExpress O95573; -.
Bgee O95573; -.
CleanEx HS_ACSL3; -.
GermOnline ENSG00000123983; Homo sapiens.
Ontologies
GO
GO:0005789; Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005792; Cellular component: microsome (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005741; Cellular component: mitochondrial outer membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005778; Cellular component: peroxisomal membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004321; Molecular function: fatty-acyl-CoA synthase activity (traceable author statement from ProtInc).
GO:0004467; Molecular function: long-chain-fatty-acid-CoA ligase activity (inferred from electronic annotation from EC).
GO:0000287; Molecular function: magnesium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0006631; Biological process: fatty acid metabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR000873; AMP-dep_Synth/Lig.
Graphical view of domain structure.
Pfam PF00501; AMP-binding; 1.
Pfam graphical view of domain structure.
PROSITE PS00455; AMP_BINDING; 1.
Proteomic databases
PeptideAtlas O95573; -.
PRIDE O95573; -.
Genome annotation databases
Ensembl ENSG00000123983; Homo sapiens. [Contig view]
GeneID 2181; -.
KEGG hsa:2181; -.
Phylogenomic databases
HOGENOM O95573; -.
HOVERGEN O95573; -.
OMA O95573; NKFKDKR.
Other
DrugBank DB00159; Icosapent.
NextBio 8805; -.
SOURCE ACSL3; Homo sapiens.
ProtoNet O95573.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Endoplasmic reticulum; Fatty acid metabolism; Ligase; Lipid metabolism; Magnesium; Membrane; Microsome; Mitochondrion; Mitochondrion outer membrane; Nucleotide-binding; Peroxisome; Phosphoprotein; Polymorphism; Signal-anchor; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   720  720     Long-chain-fatty-acid--CoA ligase 3. PRO_0000193107
TRANSMEM   21    41  21     Signal-anchor for type III membrane protein (Potential). 
TOPO_DOM   42   720  679     Cytoplasmic (Potential). 
MOD_RES   593   593        Phosphoserine. 
MOD_RES   683   683        Phosphoserine. 
MOD_RES   688   688        Phosphothreonine. 
VARIANT   551   551  1     F -> S (in dbSNP:rs1046032 [NCBI]). VAR_026716 
CONFLICT   246   246        E -> D (in Ref. 1; BAA37142 and 2; BAB72074/BAB72139). 
Sequence information
Length: 720 AA [This is the length of the unprocessed precursor] Molecular weight: 80420 Da [This is the MW of the unprocessed precursor] CRC64: AAC4B0B4543EC8DD [This is a checksum on the sequence]
        10         20         30         40         50         60 
MNNHVSSKPS TMKLKHTINP ILLYFIHFLI SLYTILTYIP FYFFSESRQE KSNRIKAKPV 

        70         80         90        100        110        120 
NSKPDSAYRS VNSLDGLASV LYPGCDTLDK VFTYAKNKFK NKRLLGTREV LNEEDEVQPN 

       130        140        150        160        170        180 
GKIFKKVILG QYNWLSYEDV FVRAFNFGNG LQMLGQKPKT NIAIFCETRA EWMIAAQACF 

       190        200        210        220        230        240 
MYNFQLVTLY ATLGGPAIVH ALNETEVTNI ITSKELLQTK LKDIVSLVPR LRHIITVDGK 

       250        260        270        280        290        300 
PPTWSEFPKG IIVHTMAAVE ALGAKASMEN QPHSKPLPSD IAVIMYTSGS TGLPKGVMIS 

       310        320        330        340        350        360 
HSNIIAGITG MAERIPELGE EDVYIGYLPL AHVLELSAEL VCLSHGCRIG YSSPQTLADQ 

       370        380        390        400        410        420 
SSKIKKGSKG DTSMLKPTLM AAVPEIMDRI YKNVMNKVSE MSSFQRNLFI LAYNYKMEQI 

       430        440        450        460        470        480 
SKGRNTPLCD SFVFRKVRSL LGGNIRLLLC GGAPLSATTQ RFMNICFCCP VGQGYGLTES 

       490        500        510        520        530        540 
AGAGTISEVW DYNTGRVGAP LVCCEIKLKN WEEGGYFNTD KPHPRGEILI GGQSVTMGYY 

       550        560        570        580        590        600 
KNEAKTKADF FEDENGQRWL CTGDIGEFEP DGCLKIIDRK KDLVKLQAGE YVSLGKVEAA 

       610        620        630        640        650        660 
LKNLPLVDNI CAYANSYHSY VIGFVVPNQK ELTELARKKG LKGTWEELCN SCEMENEVLK 

       670        680        690        700        710        720 
VLSEAAISAS LEKFEIPVKI RLSPEPWTPE TGLVTDAFKL KRKELKTHYQ ADIERMYGRK 

O95573 in FASTA format

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