[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CONJUGATION TO ATG5, AND TISSUE SPECIFICITY. DOI=10.1074/jbc.273.51.33889; PubMed=9852036 [NCBI, ExPASy, EBI, Israel, Japan] Mizushima N., Sugita H., Yoshimori T., Ohsumi Y.; "A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy."; J. Biol. Chem. 273:33889-33892(1998).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). TISSUE=Brain, and Ovary; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: Required for autophagy.
SUBUNIT: Conjugated to ATG5.
INTERACTION:
Q9H0Y0:ATG10; NbExp=1; IntAct=EBI-746742, EBI-1048913;
Q676U5:ATG16L1; NbExp=1; IntAct=EBI-746742, EBI-535909;
Q9H1Y0:ATG5; NbExp=1; IntAct=EBI-746742, EBI-1047414;
Q9H2U1:DHX36; NbExp=1; IntAct=EBI-746742, EBI-1047643;
Q01804:OTUD4; NbExp=1; IntAct=EBI-746742, EBI-1054396;
Q15459:SF3A1; NbExp=1; IntAct=EBI-746742, EBI-1054743;
O75533:SF3B1; NbExp=1; IntAct=EBI-746742, EBI-876542;
SUBCELLULAR LOCATION: Cytoplasm (By similarity).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Isoform ID	O94817-1
This is the isoform sequence displayed in this entry.

Name	2
Isoform ID	O94817-4
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_018104, VSP_018105.

TISSUE SPECIFICITY: Ubiquitous.
SIMILARITY: Belongs to the ATG12 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB017507; BAA36493.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC011033; AAH11033.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC012266; AAH12266.2; ALT_INIT; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_004698.2; -.

UniGene

Hs.264482

3D structure databases

ModBase

O94817.

Protein-protein interaction databases

IntAct

O94817; -.

Organism-specific databases

HIX0005105; -.

HGNC:588; ATG12.

CAB013272; -.

609608; gene. [NCBI / EBI]

PharmGKB

PA24879; -.

GeneCards

O94817.

Gene expression databases

ArrayExpress

O94817; -.

CleanEx

HS_ATG12; -.

GermOnline

ENSG00000145782; Homo sapiens.

Ontologies

GO:0043231;	Cellular component: intracellular membrane-bounded organelle (inferred from sequence or structural similarity from UniProtKB).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from UniProtKB).
GO:0000045;	Biological process: autophagosome formation (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR007242; Autophagy_prot_12.
Graphical view of domain structure.

PANTHER

PTHR13385; APG12; 1.

Pfam

PF04110; APG12; 1.
Pfam graphical view of domain structure.

BLOCKS

O94817.

Genome annotation databases

Ensembl

ENSG00000145782; Homo sapiens. [Contig view]

GeneID

9140; -.

KEGG

hsa:9140; -.

Phylogenomic databases

HOGENOM

O94817; -.

HOVERGEN

O94817; -.

Other

O94817; -.

ATG12; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Autophagy; Cytoplasm; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 140`	`140`	`Autophagy-related protein 12.`	`PRO_0000212471`
`CROSSLNK`	`140 140`		`Glycyl lysine isopeptide (Gly-Lys) (interchain with K-130 in ATG5).`
`VAR_SEQ`	`56 74`		`DILLKAVGDTPIMKTKKWA -> YLCESVLCSFPRPRSWNSL (in isoform 2).`	`VSP_018104`
`VAR_SEQ`	`75 140`		`Missing (in isoform 2).`	`VSP_018105`

Sequence information

Length: 140 AA [This is the length of the unprocessed precursor]

Molecular weight: 15113 Da [This is the MW of the unprocessed precursor]

CRC64: 109258521C2D194F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAEEPQSVLQ LPTSIAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD TKKKIDILLK 

        70         80         90        100        110        120 
AVGDTPIMKT KKWAVERTRT IQGLIDFIKK FLKLVASEQL FIYVNQSFAP SPDQEVGTLY 

       130        140 
ECFGSDGKLV LHYCKSQAWG

O94817 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools