ID   PLR2_SCHPO              Reviewed;         333 AA.
AC   O94521;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-NOV-2008, entry version 38.
DE   RecName: Full=Probable pyridoxal reductase 2;
DE            Short=PL reductase 2;
DE            Short=PL-red 2;
DE            EC=1.1.1.65;
GN   ORFNames=SPCC1281.04;
OS   Schizosaccharomyces pombe (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales;
OC   Schizosaccharomycetaceae; Schizosaccharomyces.
OX   NCBI_TaxID=4896;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 38366 / 972;
RX   MEDLINE=21848401; PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M.,
RA   Collins M., Connor R., Cronin A., Davis P., Feltwell T., Fraser A.,
RA   Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G.,
RA   Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K.,
RA   James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J.,
RA   Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C.,
RA   Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E.,
RA   Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S.,
RA   Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K.,
RA   Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S.,
RA   Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B.,
RA   Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S.,
RA   Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D.,
RA   Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R.,
RA   Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B.,
RA   Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S.,
RA   Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M.,
RA   Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G.,
RA   Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J.,
RA   Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L.,
RA   Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J.,
RA   Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the
RT   fission yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Catalyzes the reduction of pyridoxal (PL) with NADPH and
CC       oxidation of pyridoxine (PN) with NADP(+) (By similarity).
CC   -!- CATALYTIC ACTIVITY: Pyridoxine + NADP(+) = pyridoxal + NADPH.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family.
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DR   EMBL; CU329672; CAA22825.1; -; Genomic_DNA.
DR   PIR; T40923; T40923.
DR   RefSeq; NP_588168.1; -.
DR   GeneID; 2539165; -.
DR   KEGG; spo:SPCC1281.04; -.
DR   NMPDR; fig|4896.1.peg.506; -.
DR   GeneDB_Spombe; SPCC1281.04; -.
DR   ArrayExpress; O94521; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0050236; F:pyridoxine 4-dehydrogenase activity; IEA:EC.
DR   GO; GO:0033554; P:cellular response to stress; IEP:GeneDB_SPombe.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001395; Aldo/ket_red.
DR   Gene3D; G3DSA:3.20.20.100; Aldo/ket_red; 1.
DR   PANTHER; PTHR11732; Aldo/ket_red; 1.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   ProDom; PD000288; Aldo/ket_red; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; FALSE_NEG.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; FALSE_NEG.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; FALSE_NEG.
PE   2: Evidence at transcript level;
KW   Complete proteome; Cytoplasm; NADP; Oxidoreductase.
FT   CHAIN         1    333       Probable pyridoxal reductase 2.
FT                                /FTId=PRO_0000310315.
FT   ACT_SITE     52     52       Proton donor (By similarity).
SQ   SEQUENCE   333 AA;  36844 MW;  D69C0BEE8735E0F2 CRC64;
     MPIVNGFKVG PIGLGLMGLT WRPKQTPIKQ AFELMNYALS QGSNYWNAGE FYGINPPTAN
     LDLLADYFEK YPKNADKVFL SVKGGTDFKT LAPHGDPESV TKSVKNALTR LRGKKKLDLF
     QCARVDHKVP IETTMKALKA FVDSGEISCV GLSEASAESI KRALAIVPIA AVETEYSLFS
     RDIEKNGILD TCTQLSIPII AYAPFCHGLL TGRVKTAEDL KDFIKAFPFL RNMDKFNPKV
     FEKNIPFLKA VEQLAQKFGM SMPEFALNFI IANGKGMIIP IPGSTTVQRA ESNLSALKKS
     LSSEQLEEAK KVLDKHQIFG LRYNKQLEST LSI
//