RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Hendra virus

[TaxID: 63330]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Henipavirus.

Virus hosts

Equus caballus (Horse) [TaxID: 9796]
Homo sapiens (Human) [TaxID: 9606]
Pteropus alecto (Black flying fox) [TaxID: 9402]
Pteropus poliocephalus (Grey-headed flying fox) [TaxID: 9403]
Pteropus scapulatus (Little red flying fox) [TaxID: 94117]

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1128/JVI.74.21.9972-9979.2000; PubMed=11024125 [NCBI, ExPASy, EBI, Israel, Japan]
Wang L.-F., Yu M., Hansson E., Pritchard L.I., Shiell B., Michalski W.P., Eaton B.T.;
"The exceptionally large genome of Hendra virus: support for creation of a new genus within the family Paramyxoviridae.";
J. Virol. 74:9972-9979(2000).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the paramyxoviruses L protein family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF017149; AAC83194.2; -; Genomic_RNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T08212; T08212.

RefSeq

NP_047113.2; -.

3D structure databases

ModBase

O89344.

Family and domain databases

InterPro

IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000830; RNA_pol_ParamyxoV; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

O89344.

Genome annotation databases

GeneID

1446468; -.

Other

ProtoNet

O89344.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2244`	`2244`	`Large structural protein.`	`PRO_0000236010`
`DOMAIN`	`715 899`	`185`	`RdRp catalytic.`
`NP_BIND`	`1840 1849`	`10`	`ATP (Potential).`

Sequence information

Length: 2244 AA [This is the length of the unprocessed precursor]

Molecular weight: 257284 Da [This is the MW of the unprocessed precursor]

CRC64: F4C29F354CEA2E2E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAHELSISDI IYPECHLDSP IVSGKLISAI EYAQLRHNQP NGDKRLTENI KINLQGKRRS 

        70         80         90        100        110        120 
VYISRQSRLG NYIRDNIKNL KEFLHVSYPE CNKSLFSLKS PGMTSKLSNI MKKSFKAYNI 

       130        140        150        160        170        180 
VSRKIIEMLQ NITRNLITQD QKDEVLGIYE QDRLSNIGKY MSQSQWYECF LFWFTIKTEM 

       190        200        210        220        230        240 
RAVIKNSQKP KFRSDSCIIH MKDNNMEIVM NPNLVCIYKN DKDGKRCYYL TPEIVLMCCD 

       250        260        270        280        290        300 
VLEGRMMIET SIKSDIKYQS LITRSNALWT FIDSLFPIMG NRIYNIVSMI EPLVLALLQL 

       310        320        330        340        350        360 
KDEARILRGA FLHHCIKEIH QELIGCGFTD QKTRSIFIDD LLSVMNIDNI HLLAEFFSFF 

       370        380        390        400        410        420 
RTFGHPILEA KTAADKVREH MLADKVLEYG PIMKAHAVFC GTIINGYRDR HRGAWPPLYL 

       430        440        450        460        470        480 
PSHASKHIIR LKNSGESLTV DDCVKNWESF CGIQFDCFME LKLDSDLSMY MKDKALSPIK 

       490        500        510        520        530        540 
EEWDSVYPRE VLNYTPPRST EPRRLVDVFV NDENFDPYNM LEYVLTGDYL TDEQFNVSYS 

       550        560        570        580        590        600 
LKEKETKQAG RLFAKMTYKM RACQVIAEAL IASGVGKYFK ENGMVKDEHE LLKTLFQLSI 

       610        620        630        640        650        660 
SSVPRGNSQG RDSEFSNNTE KSLISLKRTT GRLLNNEVPC RMNIMSALID KNQSDQKKHN 

       670        680        690        700        710        720 
ILPNTRNRHK CDNTSQTFLD YHMEFSPYKS DRMDRTETSD FSKYDDGTGT KFDTVSAFLT 

       730        740        750        760        770        780 
TDLKKFCLNW RYESMAIFAE RLDEIYGLPG FFNWMHKRLE KSVIYVADPN CPPDIGKHIN 

       790        800        810        820        830        840 
LDDTPEDDIF IHSPKGGIEG YSQKTWTIAT IPFLFLSAYE TNTRIAAIVQ GDNESIAITQ 

       850        860        870        880        890        900 
KVHPNLPYKV KKEICARQAQ LYFDRLRMNL RALGLNLKAT ETIISTHLFV YSKKIHYDGA 

       910        920        930        940        950        960 
VLSQALKSMS RCCFWSETLV DETRSACSNI STTIAKAIEN GLSRNVGYCI NVLKVIQQLL 

       970        980        990       1000       1010       1020 
ISTEFSINET LTADVTSPIS NNLDWLVTAS RIPAPIGGFN YLNLSRIFVR NIGDPVTASL 

      1030       1040       1050       1060       1070       1080 
ADLKRMIEHD LMTDKVLQKV MNQEPGDASF LDWASDPYSG NLPDSQSITK TIKNITARTI 

      1090       1100       1110       1120       1130       1140 
LRTSPNPMLK GLFHDKSFEE DLELATFLMD RRIILPRAAH EILDNSLTGA REEIAGLLDT 

      1150       1160       1170       1180       1190       1200 
TKGLIRSGLK KSGIQPKLVS RLSNHDYNQF LILNRLLSNK KRNDLISPKT CSVDLAKALR 

      1210       1220       1230       1240       1250       1260 
CHMWRDLALG RSIYGLEVPD ALEAMTGRYI TGSMECQLCD QGNTMYGWFF VPRDSQLDQV 

      1270       1280       1290       1300       1310       1320 
NKEHSSIRVP YVGSSTDERS DIKLGNVKRP TRALRSAIEL QLFIPGHTEI LKKVGMRLGT 

      1330       1340       1350       1360       1370       1380 
WLPRGVNIDI DVLKAITPVS TSNNLSHRLR DRSTQFKLPG SVLNRVSRYV NISNDNLDFR 

      1390       1400       1410       1420       1430       1440 
VEGEKVDTNL IYQQTMLLGL SVLEGKFRLR TETDDYNGIY HLHVRDNCCV KEVADIGGVN 

      1450       1460       1470       1480       1490       1500 
AELPVPEYTE VENNRLIYDP DPVSEIDCDR LSKQESKARE LDFPLWSTEE LHDVLAKTVA 

      1510       1520       1530       1540       1550       1560 
QTVLEIITKA DKDVLKQHLA IDSDDSINSL ITEFLMVDPE LFALYLGQSI SVKWAFEIHH 

      1570       1580       1590       1600       1610       1620 
RRPHGRHTMV DLLSDLISNT SKHTYKVLSN ALSHPRVFKR FVNCGLLLPT QGPYLHQQDF 

      1630       1640       1650       1660       1670       1680 
EKLSQNLLIT SYMNYLMNWC DFKKFPFLIA EQDEAVVELR EDIITSKHLC MIIDLYANHH 

      1690       1700       1710       1720       1730       1740 
KPPWIIDLNP QEKICVLRDF ISKCRHTDVS SRSWNITDLD FMVFYASLTY LRRGIIKQLR 

      1750       1760       1770       1780       1790       1800 
IRQVTEVIDT TTMLRDNILV ENPPIKTGVL DIRGCIIYNL EEILSMNTKS TSRKVFNLGS 

      1810       1820       1830       1840       1850       1860 
KLSVENHKYR RIGLNSSSCY KALNLSPLIQ RYLPAGSQRL FVGEGSGSMM LLYQQTLGCS 

      1870       1880       1890       1900       1910       1920 
ISFYNSGIDG DYIPGQRELR LFPSEYSIAE DDPSQSDKLK GLVVPLFNGR PETTWIGNLD 

      1930       1940       1950       1960       1970       1980 
SYEYIINRTA GRNIGLVHSD MESGIDKQVE EIMIEHSHLI SIAINVMIED GVLVSKIAFA 

      1990       2000       2010       2020       2030       2040 
PGFPISRLLN MYRSYFGLVL VCFPVYSNPE STEVYLICLQ KTIKTIIPPQ KVLDHSYLSD 

      2050       2060       2070       2080       2090       2100 
EINDQGITSV IFKIKNIQSK QFHEDLVKHY QVEQPFFVPS HITCDEKLLM QAGLKMNGPE 

      2110       2120       2130       2140       2150       2160 
ILKNEVGYDI GSDINTLRST IIILLNEAMN YFDDERSPSH HLEPFPVLEK TRVKTIMGRV 

      2170       2180       2190       2200       2210       2220 
TRKVTVYSLI KLKETKSPEL YNIKNYIRRK VLILDFRSHT MIKLLPKGMK ERREKSGFKE 

      2230       2240 
IWIFDLSNRE VKIWWKIIGY LSLV

O89344 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools