ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry O88989

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name MDHC_RAT
Primary accession number O88989
Secondary accession numbers O88585 Q6PCV2
Integrated into Swiss-Prot on March 7, 2006
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 53)
Name and origin of the protein
Protein name Malate dehydrogenase, cytoplasmic
Synonyms EC 1.1.1.37
Cytosolic malate dehydrogenase
Gene name
Name: Mdh1
Synonyms: Mdh
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
Achenbach P., Steinbrenner H., Reindl G., Seissler J.;
"Cloning of rat cytosolic malate dehydrogenase.";
Submitted (SEP-1998) to the EMBL/GenBank/DDBJ databases.
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Pituitary;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[3]
 PROTEIN SEQUENCE OF 7-32; 80-92; 126-157; 171-199; 206-230; 239-255; 299-310 AND 319-334, AND MASS SPECTROMETRY.
STRAIN=Sprague-Dawley;
TISSUE=Brain, Hippocampus, and Spinal cord;
Lubec G., Afjehi-Sadat L., Diao W., Kang S.U.;
Submitted (JUL-2007) to UniProtKB.
[4]
 NUCLEOTIDE SEQUENCE [MRNA] OF 251-284.
Earley S.;
"Partial sequence of rat malate dehydrogenase (MDH) mRNA.";
Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF093773; AAC64180.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC059124; AAH59124.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF075574; AAC26799.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_150238.1; -.
UniGene Rn.13492
3D structure databases
HSSP P11708; 4MDH. [HSSP ENTRY / PDB]
SMR O88989; 1-333, 2-334.
ModBase O88989.
Protein-protein interaction databases
IntAct O88989; -.
PTM databases
PhosphoSite O88989; -.
Organism-specific databases
RGD 3072; Mdh1.
Gene expression databases
ArrayExpress O88989; -.
GermOnline ENSRNOG00000008103; Rattus norvegicus.
Family and domain databases
InterPro IPR001557; L-lactate/malate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR001252; Malate_DHase_AS.
IPR011274; Malate_DHase_NAD-dep_euk.
IPR010945; Malate_DHase_SF1.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR23382; MDH_SF1; 1.
Pfam PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000102; Lac_mal_DH; 1.
ProDom PD003052; Mal_dehydrog; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01759; MalateDH-SF1; 1.
TIGR01758; MDH_euk_cyt; 1.
PROSITE PS00068; MDH; 1.
BLOCKS O88989.
Genome annotation databases
Ensembl ENSRNOG00000008103; Rattus norvegicus. [Contig view]
GeneID 24551; -.
KEGG rno:24551; -.
NMPDR fig|10116.3.peg.10844; -.
Phylogenomic databases
HOVERGEN O88989; -.
Other
ProtoNet O88989.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Acetylation; Cytoplasm; Direct protein sequencing; NAD; Oxidoreductase; Phosphoprotein; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed (By similarity). 
CHAIN   2   334  333     Malate dehydrogenase, cytoplasmic. PRO_0000226737
NP_BIND   11    17  7     NAD (By similarity). 
NP_BIND   129   131  3     NAD (By similarity). 
ACT_SITE   187   187        Proton acceptor (By similarity). 
BINDING   92    92        Substrate (By similarity). 
BINDING   98    98        Substrate (By similarity). 
BINDING   105   105        NAD (By similarity). 
BINDING   112   112        NAD (By similarity). 
BINDING   131   131        Substrate (By similarity). 
BINDING   162   162        Substrate (By similarity). 
MOD_RES   2     2        N-acetylserine (By similarity). 
MOD_RES   210   210        Phosphotyrosine (By similarity). 
CONFLICT   251   251        S -> A (in Ref. 4; AAC26799). 
CONFLICT   276   276        N -> D (in Ref. 2; AAH59124). 
Sequence information
Length: 334 AA [This is the length of the unprocessed precursor] Molecular weight: 36483 Da [This is the MW of the unprocessed precursor] CRC64: 8F6778722A607B3C [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSEPIRVLVT GAAGQIAYSL LYSIGNGSVF GKDQPIILVL LDITPMMGVL DGVLMELQDC 

        70         80         90        100        110        120 
ALPLLQDVIA TDKEEVAFKD LDVAVLVGSM PRREGMERKD LLKANVKIFK SQGAALEKYA 

       130        140        150        160        170        180 
KKSVKVIVVG NPANTNCLTA SKSAPSIPKE NFSCLTRLDH NRAKSQIALK LGVTADDVKN 

       190        200        210        220        230        240 
VIIWGNHSST QYPDVNHAKV KLQGKEVGVY EALKDDSWLK GEFITTVQQR GAAVIKARKL 

       250        260        270        280        290        300 
SSAMSAAKAI SDHIRDIWFG TPEGEFVSMG VISDGNSYGV PDDLLYSFPV VIKNKTWKFV 

       310        320        330 
EGLPINDFSR EKMDLTAKEL TEEKETAFEF LSSA
O88989 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA) 		Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Hosted by ch flag SIB Switzerland Mirror sites: Australia Brazil Canada China Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!