NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=SU47 / 1021;
PubMed=9922248 [NCBI, ExPASy, EBI, Israel, Japan]
Aneja P., Charles T.C.;
"Poly-3-hydroxybutyrate degradation in Rhizobium (Sinorhizobium) meliloti: isolation and characterization of a gene encoding 3-hydroxybutyrate dehydrogenase.";
J. Bacteriol. 181:849-857(1999).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=1021;
DOI=10.1073/pnas.161294698; PubMed=11481431 [NCBI, ExPASy, EBI, Israel, Japan]
Finan T.M., Weidner S., Wong K., Buhrmester J., Chain P., Vorhoelter F.J., Hernandez-Lucas I., Becker A., Cowie A., Gouzy J., Golding B., Puehler A.;
"The complete sequence of the 1,683-kb pSymB megaplasmid from the N2-fixing endosymbiont Sinorhizobium meliloti.";
Proc. Natl. Acad. Sci. U.S.A. 98:9889-9894(2001).

Comments

CATALYTIC ACTIVITY: (R)-3-hydroxybutanoate + NAD⁺ = acetoacetate + NADH.
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF080548; AAD12733.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AL591985; CAC49536.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

H95983; H95983.

RefSeq

NP_437676.1; -.

3D structure databases

HSSP

Q10855; 1NFF. [HSSP ENTRY / PDB]

ModBase

O86034.

Enzyme and pathway databases

BioCyc

SMEL266834:SMB21010-MON; -.

Family and domain databases

InterPro

IPR011294; 3-OHbutyrate_DHase.
IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

TIGRFAMs

TIGR01963; PHB_DH; 1.

PROSITE

PS00061; ADH_SHORT; 1.

BLOCKS

O86034.

Genome annotation databases

1237467; -.

AL591985_GR; RB1136.

sme:SMb21010; -.

fig|266834.1.peg.5771; -.

Phylogenomic databases

HOGENOM

O86034; -.

Other

LinkHub

O86034; -.

Genome annotation databases

CMR

O86034; RB1136.

Other

ProtoNet

O86034.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; NAD; Oxidoreductase; Plasmid.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 258`	`258`	`D-beta-hydroxybutyrate dehydrogenase.`	`PRO_0000054528`
`NP_BIND`	`6 30`	`25`	`NAD (By similarity).`
`ACT_SITE`	`153 153`		`Proton acceptor (By similarity).`
`BINDING`	`140 140`		`Substrate (By similarity).`

Sequence information

Length: 258 AA [This is the length of the unprocessed precursor]

Molecular weight: 27179 Da [This is the MW of the unprocessed precursor]

CRC64: ACE9D388A4F0D0BA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTKTAVITGS TSGIGLAIAR TLAKAGANIV LNGFGAPDEI RTVTDEVAGL SSGTVLHHPA 

        70         80         90        100        110        120 
DMTKPSEIAD MMAMVADRFG GADILVNNAG VQFVEKIEDF PVEQWDRIIA VNLSSSFHTI 

       130        140        150        160        170        180 
RGAIPPMKKK GWGRIINIAS AHGLVASPFK SAYVAAKHGI MGLTKTVALE VAESGVTVNS 

       190        200        210        220        230        240 
ICPGYVLTPL VEKQIPDQAR TRGITEEQVI NEVMLKGQPT KKFITVEQVA SLALYLAGDD 

       250 
AAQITGTHVS MDGGWTAQ

O86034 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools