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UniProtKB/Swiss-Prot entry O82798


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ARR4_ARATH
Primary accession number O82798
Secondary accession numbers None
Integrated into Swiss-Prot on August 16, 2004
Sequence was last modified on November 1, 1998 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 57)
Name and origin of the protein
Protein name Two-component response regulator ARR4
Synonym Response regulator 1
Gene name
Name: ARR4
Synonyms: ATRR1, IBC7
OrderedLocusNames: At1g10470
ORFNames: T10O24.8
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
STRAIN=cv. Columbia;
DOI=10.1073/pnas.95.5.2691; PubMed=9482949 [NCBI, ExPASy, EBI, Israel, Japan]
Imamura A., Hanaki N., Umeda H., Nakamura A., Suzuki T., Ueguchi C., Mizuno T.;
"Response regulators implicated in His-to-Asp phosphotransfer signaling in Arabidopsis.";
Proc. Natl. Acad. Sci. U.S.A. 95:2691-2696(1998).
[2]
NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
STRAIN=cv. Columbia;
DOI=10.1016/S0014-5793(98)00418-9; PubMed=9607306 [NCBI, ExPASy, EBI, Israel, Japan]
Urao T., Yakubov B., Yamaguchi-Shinozaki K., Shinozaki K.;
"Stress-responsive expression of genes for two-component response regulator-like proteins in Arabidopsis thaliana.";
FEBS Lett. 427:175-178(1998).
[3]
NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INDUCTION.
STRAIN=cv. Wassilewskija;
DOI=10.1105/tpc.10.6.1009; PubMed=9634588 [NCBI, ExPASy, EBI, Israel, Japan]
Brandstatter I., Kieber J.J.;
"Two genes with similarity to bacterial response regulators are rapidly and specifically induced by cytokinin in Arabidopsis.";
Plant Cell 10:1009-1019(1998).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[5]
INDUCTION.
DOI=10.1016/S0014-5793(98)00611-5; PubMed=9662428 [NCBI, ExPASy, EBI, Israel, Japan]
Taniguchi M., Kiba T., Sakakibara H., Ueguchi C., Mizuno T., Sugiyama T.;
"Expression of Arabidopsis response regulator homologs is induced by cytokinins and nitrate.";
FEBS Lett. 429:259-262(1998).
[6]
INDUCTION.
DOI=10.1104/pp.124.4.1706; PubMed=11115887 [NCBI, ExPASy, EBI, Israel, Japan]
D'Agostino I.B., Deruere J., Kieber J.J.;
"Characterization of the response of the Arabidopsis response regulator gene family to cytokinin.";
Plant Physiol. 124:1706-1717(2000).
[7]
FUNCTION, AND INTERACTION WITH PHYTOCHROME B.
DOI=10.1126/science.1065022; PubMed=11691995 [NCBI, ExPASy, EBI, Israel, Japan]
Sweere U., Eichenberg K., Lohrmann J., Mira-Rodado V., Baeurle I., Kudla J., Nagy F., Schaefer E., Harter K.;
"Interaction of the response regulator ARR4 with phytochrome B in modulating red light signaling.";
Science 294:1108-1111(2001).
[8]
FUNCTION.
DOI=10.1105/tpc.018978; PubMed=14973166 [NCBI, ExPASy, EBI, Israel, Japan]
To J.P.C., Haberer G., Ferreira F.J., Deruere J., Mason M.G., Schaller G.E., Alonso J.M., Ecker J.R., Kieber J.J.;
"Type-A Arabidopsis response regulators are partially redundant negative regulators of cytokinin signaling.";
Plant Cell 16:658-671(2004).
Comments
  • FUNCTION: Functions as response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Type-A response regulators seem to act as negative regulators of the cytokinin signaling. Modulates red light signaling through its interaction with the phytochrome B photoreceptor.
  • SUBUNIT: Interacts with the phytochrome B photoreceptor.
  • INTERACTION:
    O80837:DBP; NbExp=3; IntAct=EBI-625213, EBI-1788073;
    P14713:PHYB; NbExp=2; IntAct=EBI-625213, EBI-300727;
  • SUBCELLULAR LOCATION: Nucleus (Probable).
  • TISSUE SPECIFICITY: Predominantly expressed in roots, and stems of young inflorescences.
  • INDUCTION: By low temperature, dehydration, abscisic acid (ABA), cytokinins (BA and zeatin), nitrate and high salinity.
  • PTM: Two-component system major event consists of a His-to-Asp phosphorelay between a sensor histidine kinase (HK) and a response regulator (RR). In plants, the His-to-Asp phosphorelay involves an additionnal intermediate named Histidine-containing phosphotransfer protein (HPt). This multistep phosphorelay consists of a His-Asp-His-Asp sequential transfer of a phosphate group between first an His and an Asp of the HK protein, followed by the transfer to a conserved His of the HPt protein and finally the transfer to an Asp in the receiver domain of the RR protein.
  • SIMILARITY: Belongs to the ARR family. Type-A subfamily.
  • SIMILARITY: Contains 1 response regulatory domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB008487; BAA34726.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB010915; BAA31143.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF057282; AAC26636.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC007067; AAD39568.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T48851; T48851.
RefSeq NP_172517.1; -.
UniGene At.22981
3D structure databases
ModBase O82798.
Protein-protein interaction databases
IntAct O82798; -.
Organism-specific databases
GeneFarm 4121; 418.
TAIR At1g10470; -.
Gene expression databases
ArrayExpress O82798; -.
GermOnline AT1G10470; Arabidopsis thaliana.
Ontologies
GO
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0030528; Molecular function: transcription regulator activity (traceable author statement from TAIR).
GO:0009736; Biological process: cytokinin mediated signaling (traceable author statement from TAIR).
GO:0009793; Biological process: embryonic development ending in seed dormancy (inferred from mutant phenotype from TAIR).
QuickGo view.
Family and domain databases
InterPro IPR001789; Sig_transdc_resp-reg_receiver.
Graphical view of domain structure.
Pfam PF00072; Response_reg; 1.
Pfam graphical view of domain structure.
ProDom PD000039; Response_reg; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00448; REC; 1.
SMART graphical view of domain structure.
PROSITE PS50110; RESPONSE_REGULATORY; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS O82798.
Genome annotation databases
GeneID 837587; -.
GenomeReviews CT485782_GR; AT1G10470.
KEGG ath:AT1G10470; -.
Other
ProtoNet O82798.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytokinin signaling pathway; Nucleus; Phosphoprotein; Transcription; Transcription regulation; Two-component regulatory system.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   259  259     Two-component response regulator ARR4. PRO_0000081425
DOMAIN   35   162  128     Response regulatory. 
COMPBIAS   179   246  68     Ser-rich. 
MOD_RES   95    95        4-aspartylphosphate (By similarity). 
Sequence information
Length: 259 AA [This is the length of the unprocessed precursor] Molecular weight: 28297 Da [This is the MW of the unprocessed precursor] CRC64: 557A4E2DA51948F5 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MARDGGVSCL RRSEMMSVGG IGGIESAPLD LDEVHVLAVD DSLVDRIVIE RLLRITSCKV 

        70         80         90        100        110        120 
TAVDSGWRAL EFLGLDNEKA SAEFDRLKVD LIITDYCMPG MTGYELLKKI KESSNFREVP 

       130        140        150        160        170        180 
VVIMSSENVL TRIDRCLEEG AQDFLLKPVK LADVKRLRSH LTKDVKLSNG NKRKLPEDSS 

       190        200        210        220        230        240 
SVNSSLPPPS PPLTISPESS PPLTVSTESS DSSPPLSPVE IFSTSPLSSP IDDEDDDVLT 

       250 
SSSEESPIRR QKMRSPGLD 

O82798 in FASTA format

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