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UniProtKB/Swiss-Prot entry O80536


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name PIF3_ARATH
Primary accession number O80536
Secondary accession number Q9SBC5
Integrated into Swiss-Prot on May 10, 2002
Sequence was last modified on November 1, 1998 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 69)
Name and origin of the protein
Protein name Phytochrome-interacting factor 3
Synonyms Phytochrome-associated protein 3
Basic helix-loop-helix protein 8
bHLH 8
AtbHLH008
Gene name
Name: BHLH8
Synonyms: PAP3, PIF3
OrderedLocusNames: At1g09530
ORFNames: F14J9.19
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
STRAIN=cv. Columbia;
DOI=10.1016/S0092-8674(00)81636-0; PubMed=9845368 [NCBI, ExPASy, EBI, Israel, Japan]
Ni M., Tepperman J.M., Quail P.H.;
"PIF3, a phytochrome-interacting factor necessary for normal photoinduced signal transduction, is a novel basic helix-loop-helix protein.";
Cell 95:657-667(1998).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
Lee J., Yi H., Shin B., Song P.-S., Choi G.;
"Identification and characterization of three phytochrome-associated proteins.";
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
Schoenbohm C., Weisshaar B.;
"Overview of the bHLH transcription factor gene family in Arabidopsis thaliana.";
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048500; PubMed=11130712 [NCBI, ExPASy, EBI, Israel, Japan]
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.;
"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
Nature 408:816-820(2000).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1071006; PubMed=11910074 [NCBI, ExPASy, EBI, Israel, Japan]
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.;
"Functional annotation of a full-length Arabidopsis cDNA collection.";
Science 296:141-145(2002).
[6]
FUNCTION.
DOI=10.1038/23500; PubMed=10466729 [NCBI, ExPASy, EBI, Israel, Japan]
Ni M., Tepperman J.M., Quail P.H.;
"Binding of phytochrome B to its nuclear signalling partner PIF3 is reversibly induced by light.";
Nature 400:781-784(1999).
[7]
FUNCTION.
DOI=10.1126/science.288.5467.859; PubMed=10797009 [NCBI, ExPASy, EBI, Israel, Japan]
Martinez-Garcia J.F., Huq E., Quail P.H.;
"Direct targeting of light signals to a promoter element-bound transcription factor.";
Science 288:859-863(2000).
[8]
GENE FAMILY ORGANIZATION, AND NOMENCLATURE.
DOI=10.1093/molbev/msg088; PubMed=12679534 [NCBI, ExPASy, EBI, Israel, Japan]
Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
"The basic helix-loop-helix transcription factor family in plants: a genome-wide study of protein structure and functional diversity.";
Mol. Biol. Evol. 20:735-747(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF100166; AAC95156.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF088280; AAC99771.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF251693; AAL55715.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC003970; AAC33213.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK117255; BAC41930.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR H86228; H86228.
RefSeq NP_172424.1; -.
NP_849626.1; -.
UniGene At.10926
3D structure databases
HSSP P25912; 1HLO. [HSSP ENTRY / PDB]
ModBase O80536.
Protein-protein interaction databases
IntAct O80536; -.
Organism-specific databases
TAIR At1g09530; -.
Gene expression databases
ArrayExpress O80536; -.
GermOnline AT1G09530; Arabidopsis thaliana.
Ontologies
GO
GO:0042802; Molecular function: identical protein binding (inferred from physical interaction from IntAct).
QuickGo view.
Family and domain databases
InterPro IPR001092; HLH_basic.
IPR011598; HLH_DNA_bd.
Graphical view of domain structure.
Gene3D G3DSA:4.10.280.10; HLH_DNA_bd; 1.
Pfam PF00010; HLH; 1.
Pfam graphical view of domain structure.
SMART SM00353; HLH; 1.
SMART graphical view of domain structure.
PROSITE PS50888; HLH; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS O80536.
Genome annotation databases
GeneID 837479; -.
GenomeReviews CT485782_GR; AT1G09530.
KEGG ath:AT1G09530; -.
NMPDR fig|3702.1.peg.1178; -.
Other
ProtoNet O80536.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; DNA-binding; Nucleus; Phytochrome signaling pathway; Transcription; Transcription regulation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   524  524     Phytochrome-interacting factor 3. PRO_0000127428
DOMAIN   357   393  37     Helix-loop-helix motif. 
DNA_BIND   340   356  17     Basic motif. 
COMPBIAS   353   358  6     Poly-Arg. 
COMPBIAS   422   428  7     Poly-Ala. 
CONFLICT   15    15        E -> D (in Ref. 2; AAC99771). 
CONFLICT   344   344        S -> L (in Ref. 2; AAC99771). 
Sequence information
Length: 524 AA [This is the length of the unprocessed precursor] Molecular weight: 56990 Da [This is the MW of the unprocessed precursor] CRC64: 1044AC01D598DE7C [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPLFELFRLT KAKLESAQDR NPSPPVDEVV ELVWENGQIS TQSQSSRSRN IPPPQANSSR 

        70         80         90        100        110        120 
AREIGNGSKT TMVDEIPMSV PSLMTGLSQD DDFVPWLNHH PSLDGYCSDF LRDVSSPVTV 

       130        140        150        160        170        180 
NEQESDMAVN QTAFPLFQRR KDGNESAPAA SSSQYNGFQS HSLYGSDRAR DLPSQQTNPD 

       190        200        210        220        230        240 
RFTQTQEPLI TSNKPSLVNF SHFLRPATFA KTTNNNLHDT KEKSPQSPPN VFQTRVLGAK 

       250        260        270        280        290        300 
DSEDKVLNES VASATPKDNQ KACLISEDSC RKDQESEKAV VCSSVGSGNS LDGPSESPSL 

       310        320        330        340        350        360 
SLKRKHSNIQ DIDCHSEDVE EESGDGRKEA GPSRTGLGSK RSRSAEVHNL SERRRRDRIN 

       370        380        390        400        410        420 
EKMRALQELI PNCNKVDKAS MLDEAIEYLK SLQLQVQIMS MASGYYLPPA VMFPPGMGHY 

       430        440        450        460        470        480 
PAAAAAMAMG MGMPYAMGLP DLSRGGSSVN HGPQFQVSGM QQQPVAMGIP RVSGGGIFAG 

       490        500        510        520 
SSTIGNGSTR DLSGSKDQTT TNNNSNLKPI KRKQGSSDQF CGSS 

O80536 in FASTA format

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