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UniProtKB/Swiss-Prot entry O70503


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DHB12_MOUSE
Primary accession number O70503
Secondary accession numbers Q3TID1 Q3U7V9 Q542N3 Q8CI39
Integrated into Swiss-Prot on December 15, 1998
Sequence was last modified on August 1, 1998 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 65)
Name and origin of the protein
Protein name Estradiol 17-beta-dehydrogenase 12
Synonyms EC 1.1.1.62
17-beta-hydroxysteroid dehydrogenase 12
17-beta-HSD 12
3-ketoacyl-CoA reductase
KAR
EC 1.3.1.-
KIK-I
Gene name
Name: Hsd17b12
Synonyms: Kik1
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
STRAIN=BALB/c;
TISSUE=Liver;
Gambotto A., Pagliano O., Robbins P., Deleo A.;
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
STRAIN=BALB/c, C57BL/6J, and NOD;
TISSUE=Bone marrow, Cerebellum, Colon, Head, Lung, and Thymus;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
STRAIN=FVB/N-3;
TISSUE=Mammary tumor;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
TISSUE SPECIFICITY.
DOI=10.1074/jbc.M211684200; PubMed=12482854 [NCBI, ExPASy, EBI, Israel, Japan]
Moon Y.-A., Horton J.D.;
"Identification of two mammalian reductases involved in the two-carbon fatty acyl elongation cascade.";
J. Biol. Chem. 278:7335-7343(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF064635; AAC16885.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK078841; BAC37418.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK081869; BAC38354.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK082715; BAC38583.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK088521; BAC40401.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK150849; BAE29906.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK152489; BAE31260.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK166027; BAE38530.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK167908; BAE39915.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC037620; AAH37620.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_062631.1; -.
UniGene Mm.22505
3D structure databases
HSSP P50163; 2AE1. [HSSP ENTRY / PDB]
ModBase O70503.
Protein-protein interaction databases
IntAct O70503; -.
PTM databases
PhosphoSite O70503; -.
Organism-specific databases
MGI MGI:1926967; Hsd17b12.
Gene expression databases
ArrayExpress O70503; -.
CleanEx MM_HSD17B12; -.
GermOnline ENSMUSG00000027195; Mus musculus.
Family and domain databases
InterPro IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
PANTHER PTHR19410; ADH_short_C2; 1.
Pfam PF00106; adh_short; 1.
Pfam graphical view of domain structure.
PRINTS PR00081; GDHRDH.
PR00080; SDRFAMILY.
PROSITE PS00061; ADH_SHORT; 1.
BLOCKS O70503.
Genome annotation databases
Ensembl ENSMUSG00000027195; Mus musculus. [Contig view]
GeneID 56348; -.
KEGG mmu:56348; -.
Phylogenomic databases
HOVERGEN O70503; -.
Other
LinkHub O70503; -.
SOURCE Hsd17b12; Mus musculus.
ProtoNet O70503.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Endoplasmic reticulum; Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   312  312     Estradiol 17-beta-dehydrogenase 12. PRO_0000054576
TRANSMEM   4    24  21     Potential. 
TRANSMEM   182   202  21     Potential. 
TRANSMEM   269   285  17     Potential. 
NP_BIND   50    79  30     NADP (By similarity). 
MOTIF   308   312  5     Di-lysine motif (By similarity). 
ACT_SITE   202   202        Proton acceptor (By similarity). 
BINDING   189   189        Substrate (By similarity). 
VAR_SEQ   1   135        Missing (in isoform 2). VSP_020255
CONFLICT   2     2        E -> V (in Ref. 2; BAE39915). 
CONFLICT   256   256        V -> M (in Ref. 2; BAE29906/BAE31260). 
Sequence information
Length: 312 AA [This is the length of the unprocessed precursor] Molecular weight: 34742 Da [This is the MW of the unprocessed precursor] CRC64: 7D255188FA1F8DDB [This is a checksum on the sequence]
        10         20         30         40         50         60 
MECAPPAAGF LYWVGASTIA YLALRASYSL FRAFQVWCVG NEALVGPRLG EWAVVTGGTD 

        70         80         90        100        110        120 
GIGKAYAEEL AKRGMKIVLI SRSQDKLNQV SNNIKEKFNV ETRTIAVDFS LDDIYDKIKT 

       130        140        150        160        170        180 
GLSGLEIGVL VNNVGMSYEY PEYFLEIPDL DNTIKKLINI NVLSVCKVTR LVLPGMVERS 

       190        200        210        220        230        240 
KGVILNISSA SGMLPVPLLT IYSATKAFVD FFSQCLHEEY KSKGIFVQSV MPYLVATKLA 

       250        260        270        280        290        300 
KIQKPTLDKP SAETFVKSAI KTVGLQTRTT GYVIHSLMGS INSIMPRWMY FKIIMGFSKS 

       310 
LRNRYLKKRK KN 

O70503 in FASTA format

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