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UniProtKB/Swiss-Prot entry O60573

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IF4E2_HUMAN
Primary accession number O60573
Secondary accession number O75349
Integrated into Swiss-Prot on April 11, 2003
Sequence was last modified on August 1, 1998 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 77)
Name and origin of the protein
Protein name Eukaryotic translation initiation factor 4E type 2
Synonyms eIF-4E type 2
eIF4E type 2
mRNA cap-binding protein type 3
Eukaryotic translation initiation factor 4E-like 3
Eukaryotic translation initiation factor 4E homologous protein
mRNA cap-binding protein 4EHP
eIF4E-like protein 4E-LP
Gene name
Name: EIF4E2
Synonyms: EIF4EL3
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, 3D-STRUCTURE MODELING, AND MUTAGENESIS.
TISSUE=Follicular cell;
DOI=10.1074/jbc.273.21.13104; PubMed=9582349 [NCBI, ExPASy, EBI, Israel, Japan]
Rom E., Kim H.C., Gingras A.-C., Marcotrigiano J., Favre D., Olsen H., Burley S.K., Sonenberg N.;
"Cloning and characterization of 4EHP, a novel mammalian eIF4E-related cap-binding protein.";
J. Biol. Chem. 273:13104-13109(1998).
[2]
 NUCLEOTIDE SEQUENCE [MRNA], AND INTERACTION WITH EIF4EBP1; EIF4EBP2 AND EIF4EBP3.
TISSUE=Mammary gland;
DOI=10.1111/j.1432-1033.2004.04149.x; PubMed=15153109 [NCBI, ExPASy, EBI, Israel, Japan]
Joshi B., Cameron A., Jagus R.;
"Characterization of mammalian eIF4E-family members.";
Eur. J. Biochem. 271:2189-2203(2004).
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Umbilical cord blood;
DOI=10.1073/pnas.95.14.8175; PubMed=9653160 [NCBI, ExPASy, EBI, Israel, Japan]
Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z.;
"Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning.";
Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998).
[4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Muscle, Urinary bladder, and Uterus;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
 IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
Colinge J., Superti-Furga G., Bennett K.L.;
Submitted (OCT-2008) to UniProtKB.
Comments
  • FUNCTION: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.
  • SUBUNIT: eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least eIF4A, eIF4E and eIF4G. eIF4E is also known to interact with other partners (By similarity). EIF4E2 interacts with EIF4EBP1, EIF4EBP2 and EIF4EBP3 but not with eIF4G.
  • INTERACTION:
    Q13541:EIF4EBP1; NbExp=1; IntAct=EBI-398610, EBI-74090;
    Q9Y4K3:TRAF6; NbExp=1; IntAct=EBI-398610, EBI-359276;
  • SIMILARITY: Belongs to the eukaryotic initiation factor 4E family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF047695; AAC18565.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF068117; AAC19374.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF038957; AAC39871.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC005392; AAH05392.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC005874; AAH05874.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC021226; AAH21226.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC021690; AAH21690.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00744211; -.
RefSeq NP_004837.1; -.
UniGene Hs.292026
3D structure databases
PDB
2JGB; X-ray; 1.70 A; A=45-234.[ExPASy / RCSB / EBI]
2JGC; X-ray; 2.40 A; A=45-234.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 2JGB; -.
2JGC; -.
ModBase O60573.
Protein-protein interaction databases
IntAct O60573; 7.
Organism-specific databases
GeneCards GC02P233123; -.
H-InvDB HIX0002926; -.
HIX0059993; -.
HGNC HGNC:3293; EIF4E2.
GenAtlas EIF4E2.
HPA HPA019253; -.
MIM 605895; gene. [NCBI / EBI]
PharmGKB PA27720; -.
Gene expression databases
ArrayExpress O60573; -.
Bgee O60573; -.
CleanEx HS_EIF4E2; -.
GermOnline ENSG00000135930; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from InterPro).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0000339; Molecular function: RNA cap binding (traceable author statement from ProtInc).
GO:0003743; Molecular function: translation initiation factor activity (inferred from electronic annotation from UniProtKB-KW).
GO:0006417; Biological process: regulation of translation (inferred from electronic annotation from UniProtKB-KW).
GO:0006413; Biological process: translational initiation (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001040; TIF_eIF_4E.
IPR019770; TIF_eIF_4E_CS.
Graphical view of domain structure.
Gene3D G3DSA:3.30.760.10; TIF_eIF_4E; 1.
PANTHER PTHR11960; TIF_eIF_4E; 1.
Pfam PF01652; IF4E; 1.
Pfam graphical view of domain structure.
ProDom PD003697; TIF_eIF_4E; 1.
[Domain structure / List of seq. sharing at least 1 domain]
PROSITE PS00813; IF4E; 1.
Proteomic databases
PRIDE O60573; -.
Genome annotation databases
Ensembl ENSG00000135930; Homo sapiens. [Contig view]
GeneID 9470; -.
KEGG hsa:9470; -.
Phylogenomic databases
HOVERGEN O60573; -.
OMA O60573; LWKPRLN.
Other
NextBio 35490; -.
SOURCE EIF4E2; Homo sapiens.
ProtoNet O60573.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Initiation factor; Protein biosynthesis; RNA-binding; Translation regulation.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   245  245     Eukaryotic translation initiation factor 4E type 2. PRO_0000193664
MUTAGEN   63    63        W->A: Unable to bind capped mRNA. 
MUTAGEN   95    95        W->A: Ability to bind capped mRNA reduced to 40% of wild-type. 
MUTAGEN   124   126        WED->FAA: Unable to bind capped mRNA. 
MUTAGEN   124   124        W->A: Ability to bind capped mRNA reduced to less than 10% of wild-type. 
MUTAGEN   124   124        W->F: Ability to bind capped mRNA reduced to 13% of wild-type. 
MUTAGEN   125   125        E->A: Ability to bind capped mRNA reduced to less than 10% of wild-type. 
MUTAGEN   126   126        D->A: Slight reduction in ability to bind capped mRNA. 
MUTAGEN   135   135        W->A: Unable to bind capped mRNA. 
MUTAGEN   148   148        W->A: Unable to bind capped mRNA. 
MUTAGEN   183   183        W->A: Ability to bind capped mRNA reduced to less than 10% of wild-type. 
MUTAGEN   183   183        W->F: Unable to bind capped mRNA. 
CONFLICT   1    27        MNNKFDALKDDDSGDHDQNEENSTQKD -> MMTVGTMIRMKKTAHRKI (in Ref. 3). 
STRAND   55    67  13      
HELIX   75    81  7      
STRAND   82    90  9      
HELIX   91    98  8      
HELIX   104   106  3      
STRAND   109   117  9      
TURN   127   131  5      
STRAND   133   139  7      
HELIX   144   156  13      
STRAND   166   173  8      
STRAND   178   185  8      
HELIX   190   203  14      
STRAND   212   216  5      
HELIX   217   222  6      
Sequence information
Length: 245 AA [This is the length of the unprocessed precursor] Molecular weight: 28362 Da [This is the MW of the unprocessed precursor] CRC64: 3D3075BFA48B3C12 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MNNKFDALKD DDSGDHDQNE ENSTQKDGEK EKTERDKNQS SSKRKAVVPG PAEHPLQYNY 

        70         80         90        100        110        120 
TFWYSRRTPG RPTSSQSYEQ NIKQIGTFAS VEQFWRFYSH MVRPGDLTGH SDFHLFKEGI 

       130        140        150        160        170        180 
KPMWEDDANK NGGKWIIRLR KGLASRCWEN LILAMLGEQF MVGEEICGAV VSVRFQEDII 

       190        200        210        220        230        240 
SIWNKTASDQ ATTARIRDTL RRVLNLPPNT IMEYKTHTDS IKMPGRLGPQ RLLFQNLWKP 


RLNVP
O60573 in FASTA format

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