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UniProtKB/Swiss-Prot entry O49505


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NCED2_ARATH
Primary accession number O49505
Secondary accession numbers None
Integrated into Swiss-Prot on May 1, 2007
Sequence was last modified on June 1, 1998 (Sequence version 1)
Annotations were last modified on    December 16, 2008 (Entry version 42)
Name and origin of the protein
Protein name 9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic [Precursor]
Synonyms AtNCED2
EC 1.13.11.51
Gene name
Name: NCED2
OrderedLocusNames: At4g18350
ORFNames: F28J12.10
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/47134; PubMed=10617198 [NCBI, ExPASy, EBI, Israel, Japan]
Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R.;
"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
Nature 402:769-777(1999).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
"Arabidopsis ORF clones.";
Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
[3]
FUNCTION.
PubMed=11532178 [NCBI, ExPASy, EBI, Israel, Japan]
Iuchi S., Kobayashi M., Taji T., Naramoto M., Seki M., Kato T., Tabata S., Kakubari Y., Yamaguchi-Shinozaki K., Shinozaki K.;
"Regulation of drought tolerance by gene manipulation of 9-cis-epoxycarotenoid dioxygenase, a key enzyme in abscisic acid biosynthesis in Arabidopsis.";
Plant J. 27:325-333(2001).
[4]
SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY DROUGHT STRESS.
DOI=10.1046/j.1365-313X.2003.01786.x; PubMed=12834401 [NCBI, ExPASy, EBI, Israel, Japan]
Tan B.-C., Joseph L.M., Deng W.-T., Liu L., Li Q.-B., Cline K., McCarty D.R.;
"Molecular characterization of the Arabidopsis 9-cis epoxycarotenoid dioxygenase gene family.";
Plant J. 35:44-56(2003).
[5]
TISSUE SPECIFICITY.
DOI=10.1111/j.1365-313X.2005.02622.x; PubMed=16412079 [NCBI, ExPASy, EBI, Israel, Japan]
Lefebvre V., North H., Frey A., Sotta B., Seo M., Okamoto M., Nambara E., Marion-Poll A.;
"Functional analysis of Arabidopsis NCED6 and NCED9 genes indicates that ABA synthesized in the endosperm is involved in the induction of seed dormancy.";
Plant J. 45:309-319(2006).
Comments
  • FUNCTION: Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids.
  • CATALYTIC ACTIVITY: A 9-cis-epoxycarotenoid + O2 = 2-cis,4-trans-xanthoxin + a 12'-apo-carotenal.
  • CATALYTIC ACTIVITY: 9-cis-violaxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-beta-caroten-12'-al.
  • CATALYTIC ACTIVITY: 9'-cis-neoxanthin + O2 = 2-cis,4-trans-xanthoxin + (3S,5R,6R)-5,6-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-beta-caroten-12'-al.
  • SUBCELLULAR LOCATION: Plastid, chloroplast stroma. Note=Partially bound to the thylakoid.
  • TISSUE SPECIFICITY: Very low in all tissues. Expressed at the point of organ attachment and the abscission zones in the plant.
  • INDUCTION: Low induction by drought stress.
  • SIMILARITY: Belongs to the carotenoid oxygenase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AL021710; CAA16715.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL161548; CAB78837.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT030365; ABO38778.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T04531; T04531.
RefSeq NP_193569.1; -.
UniGene At.32916
3D structure databases
ModBase O49505.
Organism-specific databases
TAIR At4g18350; -.
Gene expression databases
ArrayExpress O49505; -.
Ontologies
GO
GO:0009570; Cellular component: chloroplast stroma (inferred from direct assay from TAIR).
GO:0009535; Cellular component: chloroplast thylakoid membrane (inferred from direct assay from TAIR).
GO:0045549; Molecular function: 9-cis-epoxycarotenoid dioxygenase activity (inferred from electronic annotation from EC).
GO:0009688; Biological process: abscisic acid biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR004294; Carotenoid_Oase.
Graphical view of domain structure.
PANTHER PTHR10543; Carotenoid_Oase; 1.
Pfam PF03055; RPE65; 1.
Pfam graphical view of domain structure.
Genome annotation databases
GeneID 827562; -.
GenomeReviews CT486007_GR; AT4G18350.
KEGG ath:AT4G18350; -.
NMPDR fig|3702.1.peg.19634; -.
Other
ProtoNet O49505.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Abscisic acid biosynthesis; Chloroplast; Complete proteome; Dioxygenase; Oxidoreductase; Plastid; Transit peptide.
Features
SEVIEWER logo Feature table viewer
KeyFrom To Length Description FTId
TRANSIT   1     ?        Chloroplast (Potential). 
CHAIN   ?   583        9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic. PRO_0000285990
Sequence information
Length: 583 AA [This is the length of the unprocessed precursor] Molecular weight: 65067 Da [This is the MW of the unprocessed precursor] CRC64: A138F93542E50852 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MVSLLTMPMS GGIKTWPQAQ IDLGFRPIKR QPKVIKCTVQ IDVTELTKKR QLFTPRTTAT 

        70         80         90        100        110        120 
PPQHNPLRLN IFQKAAAIAI DAAERALISH EQDSPLPKTA DPRVQIAGNY SPVPESSVRR 

       130        140        150        160        170        180 
NLTVEGTIPD CIDGVYIRNG ANPMFEPTAG HHLFDGDGMV HAVKITNGSA SYACRFTKTE 

       190        200        210        220        230        240 
RLVQEKRLGR PVFPKAIGEL HGHSGIARLM LFYARGLCGL INNQNGVGVA NAGLVYFNNR 

       250        260        270        280        290        300 
LLAMSEDDLP YQLKITQTGD LQTVGRYDFD GQLKSAMIAH PKLDPVTKEL HALSYDVVKK 

       310        320        330        340        350        360 
PYLKYFRFSP DGVKSPELEI PLETPTMIHD FAITENFVVI PDQQVVFKLG EMISGKSPVV 

       370        380        390        400        410        420 
FDGEKVSRLG IMPKDATEAS QIIWVNSPET FCFHLWNAWE SPETEEIVVI GSCMSPADSI 

       430        440        450        460        470        480 
FNERDESLRS VLSEIRINLR TRKTTRRSLL VNEDVNLEIG MVNRNRLGRK TRFAFLAIAY 

       490        500        510        520        530        540 
PWPKVSGFAK VDLCTGEMKK YIYGGEKYGG EPFFLPGNSG NGEENEDDGY IFCHVHDEET 

       550        560        570        580 
KTSELQIINA VNLKLEATIK LPSRVPYGFH GTFVDSNELV DQL 

O49505 in FASTA format

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