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UniProtKB/Swiss-Prot entry O43837


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name IDH3B_HUMAN
Primary accession number O43837
Secondary accession numbers O95106 Q5JXS8 Q9NQ06 Q9NQ07 Q9UEX0 Q9UG99
Integrated into Swiss-Prot on July 15, 1999
Sequence was last modified on May 1, 2007 (Sequence version 2)
Annotations were last modified on    July 22, 2008 (Entry version 83)
Name and origin of the protein
Protein name Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial [Precursor]
Synonyms EC 1.1.1.41
Isocitric dehydrogenase
NAD(+)-specific ICDH
Gene name
Name: IDH3B
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), AND VARIANT VAL-3.
TISSUE=Heart;
Ko H.-J., Kim Y.-O., Park H., Oh I., Yeo S., Hong S., Chae B., Kim S., Son B., Lee Y., Yeo H., Huh T.-L.;
"Mitochondrial NAD+-specific isocitrate dehydrogenase.";
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), AND VARIANT VAL-3.
Ko H.-J., Kim Y.-O., Huh T.-L.;
Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/414865a; PubMed=11780052 [NCBI, ExPASy, EBI, Israel, Japan]
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
"The DNA sequence and comparative analysis of human chromosome 20.";
Nature 414:865-871(2001).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
TISSUE=Lung;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 127-385 (ISOFORM B).
TISSUE=Uterus;
The German cDNA consortium;
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U49283; AAB94295.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF023265; AAD09339.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF023266; AAD09340.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL049712; CAC01442.2; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL049712; CAC01443.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC001960; AAH01960.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL050094; CAB43266.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T08743; T08743.
T13147; T13147.
RefSeq NP_008830.2; -.
NP_777280.1; -.
UniGene Hs.436405
3D structure databases
HSSP P39126; 1HQS. [HSSP ENTRY / PDB]
ModBase O43837.
Protein-protein interaction databases
IntAct O43837; -.
PTM databases
PhosphoSite O43837; -.
Enzyme and pathway databases
Reactome REACT_1046; Pyruvate metabolism and TCA cycle.
Organism-specific databases
H-InvDB HIX0015578; -.
HGNC HGNC:5385; IDH3B.
GenAtlas IDH3B.
MIM 604526; gene. [NCBI / EBI]
PharmGKB PA29633; -.
GeneCards O43837.
Gene expression databases
CleanEx HS_IDH3B; -.
GermOnline ENSG00000101365; Homo sapiens.
Ontologies
GO
GO:0005759; Cellular component: mitochondrial matrix (inferred from experiment from Reactome).
GO:0009055; Molecular function: electron carrier activity (traceable author statement from UniProtKB).
GO:0004449; Molecular function: isocitrate dehydrogenase (NAD+) activity (traceable author statement from ProtInc).
GO:0006102; Biological process: isocitrate metabolic process (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR004434; IsoCit_DHase_NAD_mit.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.
Gene3D G3DSA:3.40.718.10; IDH_IMDH; 1.
PANTHER PTHR11835; IDH_IMDH_dimeric; 1.
Pfam PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.
TIGRFAMs TIGR00175; mito_nad_idh; 1.
PROSITE PS00470; IDH_IMDH; 1.
BLOCKS O43837.
Genome annotation databases
Ensembl ENSG00000101365; Homo sapiens. [Contig view]
GeneID 3420; -.
KEGG hsa:3420; -.
NMPDR fig|9606.3.peg.19736; -.
Phylogenomic databases
HOVERGEN O43837; -.
Other
DrugBank DB00157; NADH.
SOURCE IDH3B; Homo sapiens.
ProtoNet O43837.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Polymorphism; Transit peptide; Tricarboxylic acid cycle.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
TRANSIT   1    34  34     Mitochondrion (By similarity). 
CHAIN   35   385  351     Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial. PRO_0000014444
METAL   251   251        Magnesium or manganese (By similarity). 
BINDING   133   133        Substrate (By similarity). 
BINDING   164   164        Substrate (By similarity). 
BINDING   251   251        Substrate (By similarity). 
SITE   171   171  1     Critical for catalysis (By similarity). 
SITE   218   218  1     Critical for catalysis (By similarity). 
VAR_SEQ   361   385        RDMGGYSTTTDFIKSVIGHLQTKGS -> SDMGGYATCHDFTEAVIAALPHP (in isoform A). VSP_002462
VARIANT   3     3  1     A -> V (in dbSNP:rs3178817 [NCBI]). VAR_022660 
CONFLICT   127   130        SYDM -> RTLV (in Ref. 5). 
CONFLICT   385   385        S -> SNL (in Ref. 2; AAD09340). 
Sequence information
Length: 385 AA [This is the length of the unprocessed precursor] Molecular weight: 42184 Da [This is the MW of the unprocessed precursor] CRC64: 7324E6CC30A68EE2 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAALSGVRWL TRALVSAGNP GAWRGLSTSA AAHAASRSQA EDVRVEGSFP VTMLPGDGVG 

        70         80         90        100        110        120 
PELMHAVKEV FKAAAVPVEF QEHHLSEVQN MASEEKLEQV LSSMKENKVA IIGKIHTPME 

       130        140        150        160        170        180 
YKGELASYDM RLRRKLDLFA NVVHVKSLPG YMTRHNNLDL VIIREQTEGE YSSLEHESAR 

       190        200        210        220        230        240 
GVIECLKIVT RAKSQRIAKF AFDYATKKGR GKVTAVHKAN IMKLGDGLFL QCCEEVAELY 

       250        260        270        280        290        300 
PKIKFETMII DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 

       310        320        330        340        350        360 
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSSMIADAVK KVIKVGKVRT 

       370        380 
RDMGGYSTTT DFIKSVIGHL QTKGS 

O43837 in FASTA format

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