[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). PubMed=9742223 [NCBI, ExPASy, EBI, Israel, Japan] Venkateswarlu K., Gunn-Moore F., Oatey P.B., Tavare J.M., Cullen P.J.; "Nerve growth factor- and epidermal growth factor-stimulated translocation of the ADP-ribosylation factor-exchange factor GRP1 to the plasma membrane of PC12 cells requires activation of phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology domain."; Biochem. J. 335:139-146(1998).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). TISSUE=Placenta; DOI=10.1073/pnas.95.17.9926; PubMed=9707577 [NCBI, ExPASy, EBI, Israel, Japan] Franco M., Boretto J., Robineau S., Monier S., Goud B., Chardin P., Chavrier P.; "ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function."; Proc. Natl. Acad. Sci. U.S.A. 95:9926-9931(1998).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). TISSUE=Brain; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[4]	ALTERNATIVE SPLICING. DOI=10.1074/jbc.275.5.3221; PubMed=10652308 [NCBI, ExPASy, EBI, Israel, Japan] Ogasawara M., Kim S.C., Adamik R., Togawa A., Ferrans V.J., Takeda K., Kirby M., Moss J., Vaughan M.; "Similarities in function and gene structure of cytohesin-4 and cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation factors."; J. Biol. Chem. 275:3221-3230(2000).

Comments

FUNCTION: Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF through replacement of GDP with GTP.
SUBUNIT: Binds via its PH domain to the inositol head group of phosphotidylinositol 3,4,5-triphosphate with high affinity. Interacts with GRASP (By similarity).
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name 1

Isoform ID O43739-1

This is the isoform sequence displayed in this entry.

Name 2

Isoform ID O43739-2

Features which should be applied to build the isoform sequence: VSP_006039.
TISSUE SPECIFICITY: Almost absent from liver, thymus and peripheral blood lymphocytes.
SIMILARITY: Contains 1 PH domain.
SIMILARITY: Contains 1 SEC7 domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ005197; CAA06434.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ223957; CAA11686.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC028717; AAH28717.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI

IPI00013892; -.
IPI00216718; -.

RefSeq

NP_004218.1; -.

UniGene

Hs.487479

3D structure databases

HSSP

O08967; 1FHW. [HSSP ENTRY / PDB]

SMR

O43739; 59-251, 261-388.

ModBase

O43739.

Protein-protein interaction databases

IntAct

O43739; 2.

PTM databases

PhosphoSite

O43739; -.

Enzyme and pathway databases

Pathway_Interaction_DB

arf6cyclingpathway; Arf6 signaling events.
pi3kcipathway; Class I PI3K signaling events.

Organism-specific databases

GC07M006168; -.

HIX0006463; -.

HGNC:9504; CYTH3.

HPA013979; -.

605081; gene. [NCBI / EBI]

PharmGKB

PA33851; -.

Gene expression databases

O43739; -.

O43739; -.

HS_CYTH3; -.

ENSG00000008256; Homo sapiens.

Ontologies

GO:0005622;	Cellular component: intracellular (inferred from electronic annotation from InterPro).
GO:0005624;	Cellular component: membrane fraction (traceable author statement from ProtInc).
GO:0005886;	Cellular component: plasma membrane (traceable author statement from ProtInc).
GO:0005086;	Molecular function: ARF guanyl-nucleotide exchange factor activity (traceable author statement from ProtInc).
GO:0008095;	Molecular function: inositol-1,4,5-trisphosphate receptor activity (traceable author statement from ProtInc).
GO:0005545;	Molecular function: phosphatidylinositol binding (traceable author statement from ProtInc).
GO:0032012;	Biological process: regulation of ARF protein signal transduction (inferred from electronic annotation from InterPro).
GO:0016192;	Biological process: vesicle-mediated transport (traceable author statement from ProtInc).
QuickGo view.

Family and domain databases

InterPro

IPR011993; PH_type.
IPR001849; Pleckstrin_homology.
IPR000904; Sec7.
Graphical view of domain structure.

Gene3D

G3DSA:2.30.29.30; PH_type; 1.

Pfam

PF00169; PH; 1.
PF01369; Sec7; 1.
Pfam graphical view of domain structure.

SMART

SM00233; PH; 1.
SM00222; Sec7; 1.
SMART graphical view of domain structure.

PROSITE

PS50003; PH_DOMAIN; 1.
PS50190; SEC7; 1.
PROSITE graphical view of domain structure (profiles).

Proteomic databases

PRIDE

O43739; -.

Genome annotation databases

Ensembl

ENSG00000008256; Homo sapiens. [Contig view]

GeneID

9265; -.

KEGG

hsa:9265; -.

Phylogenomic databases

HOGENOM

O43739; -.

HOVERGEN

O43739; -.

OMA

O43739; WMKSIRA.

Other

34729; -.

CYTH3; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Coiled coil; Guanine-nucleotide releasing factor.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 400`	`400`	`Cytohesin-3.`	`PRO_0000120200`
`DOMAIN`	`77 206`	`130`	`SEC7.`
`DOMAIN`	`264 381`	`118`	`PH.`
`COILED`	`14 61`	`48`	`Potential.`
`VAR_SEQ`	`277 277`		`Missing (in isoform 2).`	`VSP_006039`

Sequence information

Length: 400 AA [This is the length of the unprocessed precursor]

Molecular weight: 46349 Da [This is the MW of the unprocessed precursor]

CRC64: 87FB917899EE957D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDEDGGGEGG GVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE 

        70         80         90        100        110        120 
SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL 

       130        140        150        160        170        180 
GERDEFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN 

       190        200        210        220        230        240 
PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIA MNRGINEGGD LPEELLRNLY 

       250        260        270        280        290        300 
ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT 

       310        320        330        340        350        360 
TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR VVEGNHVVYR 

       370        380        390        400 
ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK

O43739 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools