[1]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT GLY-495, FUNCTION, TISSUE SPECIFICITY, AND INTERACTION WITH WASP; PROFILIN AND ACTIN.
DOI=10.1073/pnas.94.26.14671; PubMed=9405671 [NCBI, ExPASy, EBI, Israel, Japan]
Ramesh N.,
Anton I.M.,
Hartwig J.H.,
Geha R.S.;
"WIP, a protein associated with Wiskott-Aldrich syndrome protein, induces actin polymerization and redistribution in lymphoid cells.";
Proc. Natl. Acad. Sci. U.S.A. 94:14671-14676(1997).
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[2]
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NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND VARIANT GLY-495.
TISSUE=Tonsil;
Kreideweiss S.,
Delany-Heiken P.,
Nordheim A.,
Ruhlmann A.;
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
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[3]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
TISSUE=Endometrial tumor;
The German cDNA consortium;
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
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[4]
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NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT PRO-198.
DOI=10.1038/nature03466; PubMed=15815621 [NCBI, ExPASy, EBI, Israel, Japan]
Hillier L.W.,
Graves T.A.,
Fulton R.S.,
Fulton L.A.,
Pepin K.H.,
Minx P.,
Wagner-McPherson C.,
Layman D.,
Wylie K.,
Sekhon M.,
Becker M.C.,
Fewell G.A.,
Delehaunty K.D.,
Miner T.L.,
Nash W.E.,
Kremitzki C.,
Oddy L.,
Du H.,
Sun H., ![]()
Bradshaw-Cordum H.,
Ali J.,
Carter J.,
Cordes M.,
Harris A.,
Isak A.,
van Brunt A.,
Nguyen C.,
Du F.,
Courtney L.,
Kalicki J.,
Ozersky P.,
Abbott S.,
Armstrong J.,
Belter E.A.,
Caruso L.,
Cedroni M.,
Cotton M.,
Davidson T.,
Desai A.,
Elliott G.,
Erb T.,
Fronick C.,
Gaige T.,
Haakenson W.,
Haglund K.,
Holmes A.,
Harkins R.,
Kim K.,
Kruchowski S.S.,
Strong C.M.,
Grewal N.,
Goyea E.,
Hou S.,
Levy A.,
Martinka S.,
Mead K.,
McLellan M.D.,
Meyer R.,
Randall-Maher J.,
Tomlinson C.,
Dauphin-Kohlberg S.,
Kozlowicz-Reilly A.,
Shah N.,
Swearengen-Shahid S.,
Snider J.,
Strong J.T.,
Thompson J.,
Yoakum M.,
Leonard S.,
Pearman C.,
Trani L.,
Radionenko M.,
Waligorski J.E.,
Wang C.,
Rock S.M.,
Tin-Wollam A.-M.,
Maupin R.,
Latreille P.,
Wendl M.C.,
Yang S.-P.,
Pohl C.,
Wallis J.W.,
Spieth J.,
Bieri T.A.,
Berkowicz N.,
Nelson J.O.,
Osborne J.,
Ding L.,
Meyer R.,
Sabo A.,
Shotland Y.,
Sinha P.,
Wohldmann P.E.,
Cook L.L.,
Hickenbotham M.T.,
Eldred J.,
Williams D.,
Jones T.A.,
She X.,
Ciccarelli F.D.,
Izaurralde E.,
Taylor J.,
Schmutz J.,
Myers R.M.,
Cox D.R.,
Huang X.,
McPherson J.D.,
Mardis E.R.,
Clifton S.W.,
Warren W.C.,
Chinwalla A.T.,
Eddy S.R.,
Marra M.A.,
Ovcharenko I.,
Furey T.S.,
Miller W.,
Eichler E.E.,
Bork P.,
Suyama M.,
Torrents D.,
Waterston R.H.,
Wilson R.K.;
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4.";
Nature 434:724-731(2005).
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[5]
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NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 4).
TISSUE=Skin;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
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[6]
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NUCLEOTIDE SEQUENCE [MRNA] OF 192-503 (ISOFORM 1), AND INTERACTION WITH WASP.
PubMed=10202051 [NCBI, ExPASy, EBI, Israel, Japan]
Stewart D.M.,
Tian L.,
Nelson D.L.;
"Mutations that cause the Wiskott-Aldrich syndrome impair the interaction of Wiskott-Aldrich syndrome protein (WASP) with WASP interacting protein.";
J. Immunol. 162:5019-5024(1999).
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[7]
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FUNCTION.
DOI=10.1038/35017080; PubMed=10878810 [NCBI, ExPASy, EBI, Israel, Japan]
Moreau V.,
Frischknecht F.,
Reckmann I.,
Vincentelli R.,
Rabut G.,
Stewart D.M.,
Way M.;
"A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerization.";
Nat. Cell Biol. 2:441-448(2000).
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[8]
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PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-350, AND MASS SPECTROMETRY.
DOI=10.1021/pr0705441; PubMed=18220336 [NCBI, ExPASy, EBI, Israel, Japan]
Cantin G.T.,
Yi W.,
Lu B.,
Park S.K.,
Xu T.,
Lee J.-D.,
Yates J.R. III;
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.";
J. Proteome Res. 7:1346-1351(2008).
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- FUNCTION: May have direct activity on the actin cytoskeleton. Induces actin polymerization and redistribution. Contributes with NCK1 and GRB2 in the recruitment and activation of WASL. May participate in regulating the subcellular localization of WASL, resulting in the disassembly of stress fibers in favor of filopodia formation (By similarity). Plays an important role in the intracellular motility of vaccinia virus by functioning as an adapter for recruiting WASL to vaccinia virus.
- SUBUNIT: Binds to WAS, profilin and actin. Binds to WASL (By similarity).
- INTERACTION:
Q14247:CTTN; NbExp=1; IntAct=EBI-346356, EBI-351886;
Q60598:Cttn (xeno); NbExp=2; IntAct=EBI-346356, EBI-397955;
P08631:HCK; NbExp=3; IntAct=EBI-346356, EBI-346340;
P16333:NCK1; NbExp=2; IntAct=EBI-346356, EBI-389883;
P42768:WAS; NbExp=6; IntAct=EBI-346356, EBI-346375;
- SUBCELLULAR LOCATION: Cytoplasmic vesicle (By similarity). Cytoplasm, cytoskeleton (By similarity). Note=Vesicle surfaces and along actin tails. Co-localized with actin stress fibers. When co-expressed with WASL, no longer associated with actin filaments but accumulated in perinuclear and cortical areas like WASL (By similarity).
- ALTERNATIVE PRODUCTS:
4 named isoforms [FASTA] produced by alternative splicing.
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| Name | 2 |
| Isoform ID | O43516-2 |
| Features which should be applied to build the isoform sequence: VSP_012965. |
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| Name | 3 |
| Isoform ID | O43516-3 |
| Note: No experimental confirmation available. |
| Features which should be applied to build the isoform sequence: VSP_012966. |
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| Name | 4 |
| Isoform ID | O43516-4 |
| Note: No experimental confirmation available. |
| Features which should be applied to build the isoform sequence: VSP_012964. |
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- TISSUE SPECIFICITY: Highly expressed in peripheral blood mononuclear cells, spleen, placenta, small intestin, colon, thymus. Lower expression in ovary, heart, brain, lung, liver, skeletal muscle, kidney, pancreas, prostate and testis.
- DOMAIN: Binds to WAS within the N-terminal region 170, at a site distinct from the CDC42-binding site.
- MISCELLANEOUS: Recruited to PIP5K-induced vesicle surfaces in the absence of functional WASL (By similarity).
- SIMILARITY: Belongs to the verprolin family.
- SIMILARITY: Contains 1 WH2 domain.
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