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UniProtKB/Swiss-Prot entry O43295


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SRGP2_HUMAN
Primary accession number O43295
Secondary accession numbers Q8IX13 Q8IZV8
Integrated into Swiss-Prot on July 15, 1998
Sequence was last modified on June 7, 2004 (Sequence version 3)
Annotations were last modified on    June 16, 2009 (Entry version 87)
Name and origin of the protein
Protein name SLIT-ROBO Rho GTPase-activating protein 3
Synonyms srGAP3
srGAP2
WAVE-associated Rac GTPase-activating protein
WRP
Mental disorder-associated GAP
Rho GTPase-activating protein 14
Gene name
Name: SRGAP3
Synonyms: ARHGAP14, KIAA0411, KIAA1156, MEGAP, SRGAP2
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, AND INTERACTION WITH WASF1.
DOI=10.1038/ncb886; PubMed=12447388 [NCBI, ExPASy, EBI, Israel, Japan]
Soderling S.H., Binns K.L., Wayman G.A., Davee S.M., Ong S.H., Pawson T., Scott J.D.;
"The WRP component of the WAVE-1 complex attenuates Rac-mediated signalling.";
Nat. Cell Biol. 4:970-975(2002).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), ALTERNATIVE SPLICING (ISOFORMS 2 AND 3), FUNCTION, TISSUE SPECIFICITY, AND CHROMOSOMAL TRANSLOCATION.
DOI=10.1073/pnas.162241099; PubMed=12195014 [NCBI, ExPASy, EBI, Israel, Japan]
Endris V., Wogatzky B., Leimer U., Bartsch D., Zatyka M., Latif F., Maher E.R., Tariverdian G., Kirsch S., Karch D., Rappold G.A.;
"The novel Rho-GTPase activating gene MEGAP/ srGAP3 has a putative role in severe mental retardation.";
Proc. Natl. Acad. Sci. U.S.A. 99:11754-11759(2002).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 15-1099 (ISOFORM 2).
TISSUE=Brain;
DOI=10.1093/dnares/4.5.307; PubMed=9455477 [NCBI, ExPASy, EBI, Israel, Japan]
Ishikawa K., Nagase T., Nakajima D., Seki N., Ohira M., Miyajima N., Tanaka A., Kotani H., Nomura N., Ohara O.;
"Prediction of the coding sequences of unidentified human genes. VIII. 78 new cDNA clones from brain which code for large proteins in vitro.";
DNA Res. 4:307-313(1997).
[4]
SEQUENCE REVISION.
DOI=10.1093/dnares/9.3.99; PubMed=12168954 [NCBI, ExPASy, EBI, Israel, Japan]
Nakajima D., Okazaki N., Yamakawa H., Kikuno R., Ohara O., Nagase T.;
"Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.";
DNA Res. 9:99-106(2002).
[5]
INTERACTION WITH ROBO1.
DOI=10.1016/S0092-8674(01)00530-X; PubMed=11672528 [NCBI, ExPASy, EBI, Israel, Japan]
Wong K., Ren X.R., Huang Y.Z., Xie Y., Liu G., Saito H., Tang H., Wen L., Brady-Kalnay S.M., Mei L., Wu J.Y., Xiong W.C., Rao Y.;
"Signal transduction in neuronal migration: roles of GTPase activating proteins and the small GTPase Cdc42 in the Slit-Robo pathway.";
Cell 107:209-221(2001).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-602, AND MASS SPECTROMETRY.
TISSUE=Platelet;
DOI=10.1021/pr0704130; PubMed=18088087 [NCBI, ExPASy, EBI, Israel, Japan]
Zahedi R.P., Lewandrowski U., Wiesner J., Wortelkamp S., Moebius J., Schuetz C., Walter U., Gambaryan S., Sickmann A.;
"Phosphoproteome of resting human platelets.";
J. Proteome Res. 7:526-534(2008).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-837 AND SER-858, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0805139105; PubMed=18669648 [NCBI, ExPASy, EBI, Israel, Japan]
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.;
"A quantitative atlas of mitotic phosphorylation.";
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
[8]
VARIANT [LARGE SCALE ANALYSIS] ILE-623.
DOI=10.1126/science.1133427; PubMed=16959974 [NCBI, ExPASy, EBI, Israel, Japan]
Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E.;
"The consensus coding sequences of human breast and colorectal cancers.";
Science 314:268-274(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF464189; AAN57784.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF427144; AAN07095.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB007871; BAA24841.2; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00218087; -.
IPI00412209; -.
IPI00412210; -.
RefSeq NP_001028289.1; -.
NP_055665.1; -.
UniGene Hs.654743
3D structure databases
HSSP P29354; 1AZE. [HSSP ENTRY / PDB]
SMR O43295; 749-800.
ModBase O43295.
Protein-protein interaction databases
IntAct O43295; 2.
PTM databases
PhosphoSite O43295; -.
Enzyme and pathway databases
Reactome REACT_11044; Signaling by Rho GTPases.
Organism-specific databases
GeneCards GC03M008998; -.
H-InvDB HIX0003024; -.
HIX0077832; -.
HGNC HGNC:19744; SRGAP3.
GenAtlas SRGAP3.
MIM 606525; gene. [NCBI / EBI]
PharmGKB PA134935463; -.
HUGE KIAA0411.
KIAA1156.
Gene expression databases
ArrayExpress O43295; -.
Bgee O43295; -.
CleanEx HS_SRGAP2; -.
HS_SRGAP3; -.
GermOnline ENSG00000196220; Homo sapiens.
Ontologies
GO
GO:0005622; Cellular component: intracellular (inferred from electronic annotation from InterPro).
GO:0005096; Molecular function: GTPase activator activity (inferred from electronic annotation from UniProtKB-KW).
GO:0007165; Biological process: signal transduction (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001060; FCH.
IPR000198; RhoGAP.
IPR001452; SH3_domain.
Graphical view of domain structure.
Gene3D G3DSA:1.10.555.10; RhoGAP; 1.
Pfam PF00611; FCH; 1.
PF00620; RhoGAP; 1.
PF00018; SH3_1; 1.
Pfam graphical view of domain structure.
ProDom PD000066; SH3; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00055; FCH; 1.
SM00324; RhoGAP; 1.
SM00326; SH3; 1.
SMART graphical view of domain structure.
PROSITE PS50133; FCH; 1.
PS50238; RHOGAP; 1.
PS50002; SH3; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE O43295; -.
Genome annotation databases
Ensembl ENSG00000196220; Homo sapiens. [Contig view]
GeneID 9901; -.
KEGG hsa:9901; -.
Phylogenomic databases
HOGENOM O43295; -.
HOVERGEN O43295; -.
OMA O43295; CPSSPHK.
Other
NextBio 37331; -.
SOURCE SRGAP3; Homo sapiens.
ProtoNet O43295.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Chromosomal rearrangement; Coiled coil; GTPase activation; Phosphoprotein; Polymorphism; SH3 domain.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1099  1099     SLIT-ROBO Rho GTPase-activating protein 3. PRO_0000056769
DOMAIN   22     87  66     FCH. 
DOMAIN   482    670  189     Rho-GAP. 
DOMAIN   720    779  60     SH3. 
COILED   352    392  41     Potential. 
COILED   952    987  36     Potential. 
MOD_RES   602    602        Phosphoserine. 
MOD_RES   837    837        Phosphoserine. 
MOD_RES   858    858        Phosphoserine. 
MOD_RES   1070   1070        Phosphoserine (By similarity). 
VAR_SEQ   480    513        PPCLPPKPQKMRRPRPLSVYSHKLFNGSMEAFIK -> GRRNARTRNQ (in isoform 2). VSP_010581
VAR_SEQ   480    489        PPCLPPKPQK -> IQDKLYRL (in isoform 3). VSP_010582
VAR_SEQ   490   1099        Missing (in isoform 3). VSP_010583
VARIANT   623    623  1     L -> I (in a breast cancer sample; somatic mutation). VAR_035550 
VARIANT   628    628  1     I -> V (in dbSNP:rs2271207 [NCBI]). VAR_049159 
Sequence information
Length: 1099 AA [This is the length of the unprocessed precursor] Molecular weight: 124504 Da [This is the MW of the unprocessed precursor] CRC64: 2D1BA2D338E1BF13 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSSQTKFKKD KEIIAEYEAQ IKEIRTQLVE QFKCLEQQSE SRLQLLQDLQ EFFRRKAEIE 

        70         80         90        100        110        120 
LEYSRSLEKL AERFSSKIRS SREHQFKKDQ YLLSPVNCWY LVLHQTRRES RDHATLNDIF 

       130        140        150        160        170        180 
MNNVIVRLSQ ISEDVIRLFK KSKEIGLQMH EELLKVTNEL YTVMKTYHMY HAESISAESK 

       190        200        210        220        230        240 
LKEAEKQEEK QFNKSGDLSM NLLRHEDRPQ RRSSVKKIEK MKEKRQAKYS ENKLKCTKAR 

       250        260        270        280        290        300 
NDYLLNLAAT NAAISKYYIH DVSDLIDCCD LGFHASLART FRTYLSAEYN LETSRHEGLD 

       310        320        330        340        350        360 
VIENAVDNLD SRSDKHTVMD MCNQVFCPPL KFEFQPHMGD EVCQVSAQQP VQTELLMRYH 

       370        380        390        400        410        420 
QLQSRLATLK IENEEVRKTL DATMQTLQDM LTVEDFDVSD AFQHSRSTES VKSAASETYM 

       430        440        450        460        470        480 
SKINIAKRRA NQQETEMFYF TKFKEYVNGS NLITKLQAKH DLLKQTLGEG ERAECGTTRP 

       490        500        510        520        530        540 
PCLPPKPQKM RRPRPLSVYS HKLFNGSMEA FIKDSGQAIP LVVESCIRYI NLYGLQQQGI 

       550        560        570        580        590        600 
FRVPGSQVEV NDIKNSFERG EDPLVDDQNE RDINSVAGVL KLYFRGLENP LFPKERFQDL 

       610        620        630        640        650        660 
ISTIKLENPA ERVHQIQQIL VTLPRVVIVV MRYLFAFLNH LSQYSDENMM DPYNLAICFG 

       670        680        690        700        710        720 
PTLMHIPDGQ DPVSCQAHIN EVIKTIIIHH EAIFPSPREL EGPVYEKCMA GGEEYCDSPH 

       730        740        750        760        770        780 
SEPGAIDEVD HDNGTEPHTS DEEVEQIEAI AKFDYMGRSP RELSFKKGAS LLLYHRASED 

       790        800        810        820        830        840 
WWEGRHNGVD GLIPHQYIVV QDMDDAFSDS LSQKADSEAS SGPLLDDKAS SKNDLQSPTE 

       850        860        870        880        890        900 
HISDYGFGGV MGRVRLRSDG AAIPRRRSGG DTHSPPRGLG PSIDTPPRAA ACPSSPHKIP 

       910        920        930        940        950        960 
LTRGRIESPE KRRMATFGSA GSINYPDKKA LSEGHSMRST CGSTRHSSLG DHKSLEAEAL 

       970        980        990       1000       1010       1020 
AEDIEKTMST ALHELRELER QNTVKQAPDV VLDTLEPLKN PPGPVSSEPA SPLHTIVIRD 

      1030       1040       1050       1060       1070       1080 
PDAAMRRSSS SSTEMMTTFK PALSARLAGA QLRPPPMRPV RPVVQHRSSS SSSSGVGSPA 

      1090 
VTPTEKMFPN SSADKSGTM 

O43295 in FASTA format

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