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UniProtKB/Swiss-Prot entry O34962


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MAO4_BACSU
Primary accession number O34962
Secondary accession numbers None
Integrated into Swiss-Prot on February 28, 2003
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 55)
Name and origin of the protein
Protein name Probable NAD-dependent malic enzyme 4
Synonyms NAD-ME 4
EC 1.1.1.38
Gene name
Name: ytsJ
OrderedLocusNames: BSU29220
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=168;
PubMed=9387221 [NCBI, ExPASy, EBI, Israel, Japan]
Lapidus A., Galleron N., Sorokin A., Ehrlich S.-D.;
"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.";
Microbiology 143:3431-3441(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF008220; AAC00339.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99118; CAB14882.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR C70001; C70001.
RefSeq NP_390800.1; -.
3D structure databases
ModBase O34962.
Enzyme and pathway databases
BioCyc BSUB224308:BSU2918-MON; -.
Organism-specific databases
SubtiList BG13922; ytsJ. [Micado]
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS O34962.
Genome annotation databases
GeneID 937378; -.
GenomeReviews AL009126_GR; BSU29220.
KEGG bsu:BSU29220; -.
NMPDR fig|224308.1.peg.2925; -.
Phylogenomic databases
HOGENOM O34962; -.
Genome annotation databases
CMR O34962; BSU29220.
Other
ProtoNet O34962.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   410  410     Probable NAD-dependent malic enzyme 4. PRO_0000160211
NP_BIND   73    80  8     NAD (By similarity). 
ACT_SITE   91    91        Proton acceptor (By similarity). 
METAL   133   133        Divalent metal cation (By similarity). 
METAL   134   134        Divalent metal cation (By similarity). 
BINDING   159   159        NAD (By similarity). 
BINDING   286   286        NAD (By similarity). 
Sequence information
Length: 410 AA [This is the length of the unprocessed precursor] Molecular weight: 43667 Da [This is the MW of the unprocessed precursor] CRC64: 3B9B2FDAAA349D61 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSLREEALHL HKVNQGKLES KSKVEVRNAK DLSLAYSPGV AEPCKDIHED INKVYDYTMK 

        70         80         90        100        110        120 
GNMVAVVTDG TAVLGLGNIG PEAALPVMEG KAVLFKSFAG VDAFPIALNT NDVDKIVETV 

       130        140        150        160        170        180 
KLLEPTFGGV NLEDIAAPNC FIIEERLKKE TNIPVFHDDQ HGTAIVTVAG LVNALKLSGK 

       190        200        210        220        230        240 
SMSSIKVVAN GAGAAGIAII KLLHHYGVRD IVMCDSKGAI YEGRPNGMND VKNEVAKFTN 

       250        260        270        280        290        300 
QDRKDGSLKD VIVDADVFIG VSVAGALTKE MVQSMAKDPI IFAMANPNPE IMPEDAREAG 

       310        320        330        340        350        360 
ASVVGTGRSD FPNQVNNVLA FPGIFRGALD VRATHINEEM KIAAVEAIAS LVSEDELSAD 

       370        380        390        400        410 
YVIPAPFDKR VAPAVAKAVA KAAMETGVAR ITVDPEEVAE KTRKLTIIGE 

O34962 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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