ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry O34389


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name MAO3_BACSU
Primary accession number O34389
Secondary accession numbers None
Integrated into Swiss-Prot on February 28, 2003
Sequence was last modified on January 1, 1998 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 56)
Name and origin of the protein
Protein name Probable NAD-dependent malic enzyme 3
Synonyms NAD-ME 3
EC 1.1.1.38
Gene name
Name: malS
OrderedLocusNames: BSU29880
From
Bacillus subtilis [TaxID: 1423] [HAMAP proteome]
Taxonomy Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
Protein existence 3: Inferred from homology;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=9387221 [NCBI, ExPASy, EBI, Israel, Japan]
Lapidus A., Galleron N., Sorokin A., Ehrlich S.-D.;
"Sequencing and functional annotation of the Bacillus subtilis genes in the 200 kb rrnB-dnaB region.";
Microbiology 143:3431-3441(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=168;
DOI=10.1038/36786; PubMed=9384377 [NCBI, ExPASy, EBI, Israel, Japan]
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.;
"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis.";
Nature 390:249-256(1997).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF008220; AAC00287.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z99119; CAB14966.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR D69655; D69655.
RefSeq NP_390866.1; -.
3D structure databases
HSSP P40927; 1GQ2. [HSSP ENTRY / PDB]
ModBase O34389.
Enzyme and pathway databases
BioCyc BSUB224308:BSU2984-MON; -.
Organism-specific databases
SubtiList BG12614; malS. [Micado]
Family and domain databases
InterPro IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR001891; Malic_OxRdtase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.
Gene3D G3DSA:3.40.50.720; NAD(P)-bd; 1.
Pfam PF00390; malic; 1.
PF03949; Malic_M; 1.
Pfam graphical view of domain structure.
PRINTS PR00072; MALOXRDTASE.
PROSITE PS00331; MALIC_ENZYMES; 1.
BLOCKS O34389.
Genome annotation databases
GeneID 938071; -.
GenomeReviews AL009126_GR; BSU29880.
KEGG bsu:BSU29880; -.
NMPDR fig|224308.1.peg.2991; -.
Phylogenomic databases
HOGENOM O34389; -.
Genome annotation databases
CMR O34389; BSU29880.
Other
ProtoNet O34389.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Metal-binding; NAD; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   566  566     Probable NAD-dependent malic enzyme 3. PRO_0000160210
ACT_SITE   105   105        Proton donor (By similarity). 
ACT_SITE   178   178        Proton acceptor (By similarity). 
METAL   249   249        Divalent metal cation (By similarity). 
METAL   250   250        Divalent metal cation (By similarity). 
METAL   273   273        Divalent metal cation (By similarity). 
BINDING   273   273        NAD (By similarity). 
BINDING   423   423        NAD (By similarity). 
SITE   273   273  1     Important for activity (By similarity). 
Sequence information
Length: 566 AA [This is the length of the unprocessed precursor] Molecular weight: 62149 Da [This is the MW of the unprocessed precursor] CRC64: 226C658154548270 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKQFRVTNEG DIQTTLRGLE VLSVPFLNKG VAFTEEERKE LGLKGFLPPK VLTIDDQAKR 

        70         80         90        100        110        120 
AYEQYSAQPD DLSKNVYLTA LHDRNETLFY RLLNDHLGEM LPIVYTPTVG TAIQRYSHEY 

       130        140        150        160        170        180 
RKPRGLYLSI DDPDGMKEAF KQYKDQSDTI DLIVATDAEG ILGIGDWGVG GIAISIGKLA 

       190        200        210        220        230        240 
VYTAAAGIDP SRVLAVVLDA GTNQESLLND PLYVGNQHSR VRGERYDQFI DDYVALARET 

       250        260        270        280        290        300 
FPNALLHWED FGAKNARSIL KRYKDKVCTF NDDIQGTGAV SLAAVLSCAK ASKVPLRDHR 

       310        320        330        340        350        360 
VVIFGAGTAG IGIAEQLREA LVREGLSEEE SYKRFWCIDR NGLLTDDMDQ LLDFQKPYAR 

       370        380        390        400        410        420 
SADEVKDYQR NGDGGGIDLL EVVRQAKPTI LIGTSTVSGA FTEEIVKEMA SHVKRPAILP 

       430        440        450        460        470        480 
MSNPTTLSEA KPEDLIEWTE GRALITTGSP FPPVEYNGVT YHIGQANNAL VFPGLGLGTI 

       490        500        510        520        530        540 
VTKSKLITDG MFEACARAIA GMVNVGVPGA PMLPKVEDLR TVSATVAVEV AKTAMKEGVA 

       550        560 
TEEPEDIIQA VQDAMWYPVY KPIRAI 

O34389 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ch flag SIB Switzerland Mirror sites: Australia  Brazil  Canada  China  Korea
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!