[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION. STRAIN=RCR2011 / SU47; DOI=10.1074/jbc.273.15.9330; PubMed=9535928 [NCBI, ExPASy, EBI, Israel, Japan] Mitsch M.J., Voegele R.T., Cowie A., Oesteras M., Finan T.M.; "Chimeric structure of the NAD(P)+- and NADP+-dependent malic enzymes of Rhizobium (Sinorhizobium) meliloti."; J. Biol. Chem. 273:9330-9336(1998).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=1021; DOI=10.1073/pnas.161294398; PubMed=11481430 [NCBI, ExPASy, EBI, Israel, Japan] Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D., Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.; "Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021."; Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001).
[3]	CHARACTERIZATION. DOI=10.1016/S0167-4838(99)00112-0; PubMed=10407149 [NCBI, ExPASy, EBI, Israel, Japan] Voegele R.T., Mitsch M.J., Finan T.M.; "Characterization of two members of a novel malic enzyme class."; Biochim. Biophys. Acta 1432:275-285(1999).

Comments

FUNCTION: Required for symbiotic nitrogen fixation. Plays a key role in the conversion of malate to acetyl-CoA for efficient tricarboxylic acid cycle function in nitrogen-fixating bacteria.
CATALYTIC ACTIVITY: (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH.
COFACTOR: Divalent metal cations. Prefers magnesium or manganese (By similarity).
ENZYME REGULATION: Subject to substrate inhibition and shows allosteric regulation by acetyl-CoA.
SUBUNIT: Homooctamer.
SIMILARITY: In the N-terminal section; belongs to the malic enzymes family.
SIMILARITY: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF017443; AAB82459.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AL591688; CAC46416.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_385943.1; -.

3D structure databases

ModBase

O30807.

Enzyme and pathway databases

BioCyc

SMEL266834:SMC00169-MON; -.

Family and domain databases

InterPro

IPR015884; Malic_enzyme_CS.
IPR012301; Malic_N.
IPR012302; Malic_NAD_bd.
IPR012188; ME_PTA.
IPR016040; NAD(P)-bd.
IPR002505; PTA_PTB.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF00390; malic; 1.
PF03949; Malic_M; 1.
PF01515; PTA_PTB; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF036684; ME_PTA; 1.

PROSITE

PS00331; MALIC_ENZYMES; 1.

BLOCKS

O30807.

Genome annotation databases

1233502; -.

AL591688_GR; R01837.

sme:SMc00169; -.

fig|266834.1.peg.3131; -.

Phylogenomic databases

HOGENOM

O30807; -.

Genome annotation databases

CMR

O30807; R01837.

Other

ProtoNet

O30807.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Allosteric enzyme; Complete proteome; Metal-binding; Multifunctional enzyme; NAD; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 770`	`770`	`NAD-dependent malic enzyme.`	`PRO_0000160245`
`REGION`	`1 440`	`440`	`Malic enzyme.`
`REGION`	`441 770`	`330`	`Phosphate acetyltransferase.`
`ACT_SITE`	`107 107`		`Proton acceptor (By similarity).`
`METAL`	`149 149`		`Divalent metal cation (By similarity).`
`METAL`	`150 150`		`Divalent metal cation (By similarity).`
`BINDING`	`175 175`		`NAD (By similarity).`
`BINDING`	`300 300`		`NAD (By similarity).`

Sequence information

Length: 770 AA [This is the length of the unprocessed precursor]

Molecular weight: 82865 Da [This is the MW of the unprocessed precursor]

CRC64: 8FF3E3DA2D923108 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNTGDKAKSQ AVPASGDIDQ QALFFHRYPR PGKLEIQPTK PLGNQRDLAL AYSPGVAAPC 

        70         80         90        100        110        120 
LAIKDNPETA ADFTARANLV AVVSNGTAVL GLGNIGPLAS KPVMEGKAVL FKKFAGIDVF 

       130        140        150        160        170        180 
DIEIDAPTVD RMVDVISALE PTFGGINLED IKAPECFEVE RRLREKMEIP VFHDDQHGTA 

       190        200        210        220        230        240 
IIVAAAVLNG LELAGKDIAE AKIVASGAGA AALACLNLLV TLGARRENIW VHDIEGLVYK 

       250        260        270        280        290        300 
GREALMDEWK AVYAQESDNR VLADSIGGAD VFLGLSAAGV LKPELLARMA EKPLIMALAN 

       310        320        330        340        350        360 
PTPEIMPEVA RAARPDAMIC TGRSDFPNQV NNVLCFPHIF RGALDCGART INEEMKMAAV 

       370        380        390        400        410        420 
RAIAGLAREE PSDVAARAYS GETPVFGPDY LIPSPFDQRL ILRIAPAVAK AAAESGVATR 

       430        440        450        460        470        480 
PIQDFDAYLD KLNRFVFRSG FIMKPVFAAA KNAAKNRVIF AEGEDERVLR AAQVLLEEGT 

       490        500        510        520        530        540 
AKPILIGRPQ IIETRLRRYG LRIRPDVDFE VVNPEGDPRY RDYVDDYFAL VGRLGVIPEA 

       550        560        570        580        590        600 
ARTIVRTNTT VIGALAVKRG EADALICGVE GRYSRHLRDV SQIIGKRSGV LDFSALSLLI 

       610        620        630        640        650        660 
SQRGATFFTD TYVSFSPSAE EIAQTTVMAA NEIRRFGITP RAALVSHSNF GSRDSESAFK 

       670        680        690        700        710        720 
MRTALQLVRE LAPDLEVDGE MHGDSAISEV LRQRVMPDST LNGEANLLVF PNLDAANITL 

       730        740        750        760        770 
GVVKTMTDSL HVGPILLGSA LPAHILSPSV TSRGVVNMAA LAVVESSHPV

O30807 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools